An integron of class 1 is present on the plasmid pCG4 from Gram-positive bacterium Corynebacterium glutamicum
Abstract The streptomycin/spectinomycin resistance determinant of the 29-kb plasmid pCG4 from Corynebacterium glutamicum was found to be a part of a typical class 1 integron. The sequence analysis revealed that the integron (designated InCg) identified in this Gram-positive bacterium is almost ident...
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| Veröffentlicht in: | FEMS microbiology letters 1998-12, Vol.169 (2), p.391-395 |
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| creator | NESVERA, J HOCHMANNOVA, J PATEK, M |
| description | Abstract
The streptomycin/spectinomycin resistance determinant of the 29-kb plasmid pCG4 from Corynebacterium glutamicum was found to be a part of a typical class 1 integron. The sequence analysis revealed that the integron (designated InCg) identified in this Gram-positive bacterium is almost identical to the integron InC present on the plasmid pSA1700 from the Gram-negative bacterium Pseudomonas aeruginosa. Differences in only two base pairs were found in the 3.8-kb sequence. One base substitution (G→C) is present in the streptomycin/spectinomycin resistance determinant which is thus identical to the aadA2a gene from the integron In6 of the broad-host-range plasmid pSa. The other one (C→G) is present in the extended −10 region of the integron promoter involved in expression of the antibiotic resistance gene. It was shown that this novel version of the integron promoter displays five times higher activity in both C. glutamicum and Escherichia coli than the original one. |
| doi_str_mv | 10.1111/j.1574-6968.1998.tb13345.x |
| format | Article |
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The streptomycin/spectinomycin resistance determinant of the 29-kb plasmid pCG4 from Corynebacterium glutamicum was found to be a part of a typical class 1 integron. The sequence analysis revealed that the integron (designated InCg) identified in this Gram-positive bacterium is almost identical to the integron InC present on the plasmid pSA1700 from the Gram-negative bacterium Pseudomonas aeruginosa. Differences in only two base pairs were found in the 3.8-kb sequence. One base substitution (G→C) is present in the streptomycin/spectinomycin resistance determinant which is thus identical to the aadA2a gene from the integron In6 of the broad-host-range plasmid pSa. The other one (C→G) is present in the extended −10 region of the integron promoter involved in expression of the antibiotic resistance gene. It was shown that this novel version of the integron promoter displays five times higher activity in both C. glutamicum and Escherichia coli than the original one.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1998.tb13345.x</identifier><identifier>PMID: 9868786</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antibiotic resistance ; Antibiotics ; Bacteria ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Corynebacterium - drug effects ; Corynebacterium - genetics ; Corynebacterium glutamicum ; Drug Resistance, Microbial ; E coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genetics ; Integrases - genetics ; Integron ; Microbiology ; Molecular Sequence Data ; Plasmids - genetics ; Promoter ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - genetics ; Spectinomycin ; Streptomycin ; Streptomycin - pharmacology</subject><ispartof>FEMS microbiology letters, 1998-12, Vol.169 (2), p.391-395</ispartof><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 1998</rights><rights>1999 INIST-CNRS</rights><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3501-e99759fb5dfcb183309780e8a811491a4624669860147c3270dbf1aca03db0083</citedby><cites>FETCH-LOGICAL-c3501-e99759fb5dfcb183309780e8a811491a4624669860147c3270dbf1aca03db0083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1998.tb13345.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1998.tb13345.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1664015$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9868786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NESVERA, J</creatorcontrib><creatorcontrib>HOCHMANNOVA, J</creatorcontrib><creatorcontrib>PATEK, M</creatorcontrib><title>An integron of class 1 is present on the plasmid pCG4 from Gram-positive bacterium Corynebacterium glutamicum</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
The streptomycin/spectinomycin resistance determinant of the 29-kb plasmid pCG4 from Corynebacterium glutamicum was found to be a part of a typical class 1 integron. The sequence analysis revealed that the integron (designated InCg) identified in this Gram-positive bacterium is almost identical to the integron InC present on the plasmid pSA1700 from the Gram-negative bacterium Pseudomonas aeruginosa. Differences in only two base pairs were found in the 3.8-kb sequence. One base substitution (G→C) is present in the streptomycin/spectinomycin resistance determinant which is thus identical to the aadA2a gene from the integron In6 of the broad-host-range plasmid pSa. The other one (C→G) is present in the extended −10 region of the integron promoter involved in expression of the antibiotic resistance gene. It was shown that this novel version of the integron promoter displays five times higher activity in both C. glutamicum and Escherichia coli than the original one.</description><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Corynebacterium - drug effects</subject><subject>Corynebacterium - genetics</subject><subject>Corynebacterium glutamicum</subject><subject>Drug Resistance, Microbial</subject><subject>E coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Genetics</subject><subject>Integrases - genetics</subject><subject>Integron</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Plasmids - genetics</subject><subject>Promoter</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Spectinomycin</subject><subject>Streptomycin</subject><subject>Streptomycin - pharmacology</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkVGL1DAUhYMo67j6E4Sg4lvH3CZNEx-EZXBHYcQXfQ5pmq4ZmqYmre78e1Om7IIoYl5CON-9594chF4A2UI-b45bqGpWcMnFFqQU26kBSlm1vX2ANnfSQ7QhtBYFEFk_Rk9SOhJCWEn4BbqQgota8A3yVwN2w2RvYhhw6LDpdUoYsEt4jDbZYcJZmL5ZPGbFuxaPuz3DXQwe76P2xRiSm9wPixttJhvd7PEuxNNg7983_Txp78zsn6JHne6Tfbbel-jr9fsvuw_F4fP-4-7qUBhaESislHUlu6ZqO9OAoDRvIIgVWgAwCZrxknGedyDAakPLmrRNB9poQtuGEEEv0etz3zGG77NNk_IuGdv3erBhTopLIFV2-icINdAKapbBl7-BxzDHIS-hSkqB0VKyMlNvz5SJIaVoOzVG53U8KSBqiU4d1ZKPWvJRS3RqjU7d5uLnq8XceNvela5ZZf3VqutkdN9FPRiX7h04ZwSqjL07Yz9db0__MYC6_nSgcvmT6twgzONfyos_zf8LMKfEXA</recordid><startdate>199812</startdate><enddate>199812</enddate><creator>NESVERA, J</creator><creator>HOCHMANNOVA, J</creator><creator>PATEK, M</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199812</creationdate><title>An integron of class 1 is present on the plasmid pCG4 from Gram-positive bacterium Corynebacterium glutamicum</title><author>NESVERA, J ; HOCHMANNOVA, J ; PATEK, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3501-e99759fb5dfcb183309780e8a811491a4624669860147c3270dbf1aca03db0083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Corynebacterium - drug effects</topic><topic>Corynebacterium - genetics</topic><topic>Corynebacterium glutamicum</topic><topic>Drug Resistance, Microbial</topic><topic>E coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Genetics</topic><topic>Integrases - genetics</topic><topic>Integron</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Plasmids - genetics</topic><topic>Promoter</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Spectinomycin</topic><topic>Streptomycin</topic><topic>Streptomycin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NESVERA, J</creatorcontrib><creatorcontrib>HOCHMANNOVA, J</creatorcontrib><creatorcontrib>PATEK, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NESVERA, J</au><au>HOCHMANNOVA, J</au><au>PATEK, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An integron of class 1 is present on the plasmid pCG4 from Gram-positive bacterium Corynebacterium glutamicum</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1998-12</date><risdate>1998</risdate><volume>169</volume><issue>2</issue><spage>391</spage><epage>395</epage><pages>391-395</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
The streptomycin/spectinomycin resistance determinant of the 29-kb plasmid pCG4 from Corynebacterium glutamicum was found to be a part of a typical class 1 integron. The sequence analysis revealed that the integron (designated InCg) identified in this Gram-positive bacterium is almost identical to the integron InC present on the plasmid pSA1700 from the Gram-negative bacterium Pseudomonas aeruginosa. Differences in only two base pairs were found in the 3.8-kb sequence. One base substitution (G→C) is present in the streptomycin/spectinomycin resistance determinant which is thus identical to the aadA2a gene from the integron In6 of the broad-host-range plasmid pSa. The other one (C→G) is present in the extended −10 region of the integron promoter involved in expression of the antibiotic resistance gene. It was shown that this novel version of the integron promoter displays five times higher activity in both C. glutamicum and Escherichia coli than the original one.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9868786</pmid><doi>10.1111/j.1574-6968.1998.tb13345.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1998-12, Vol.169 (2), p.391-395 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Antibiotic resistance Antibiotics Bacteria Bacterial Proteins - genetics Bacteriology Base Sequence Biological and medical sciences Corynebacterium - drug effects Corynebacterium - genetics Corynebacterium glutamicum Drug Resistance, Microbial E coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene expression Genetics Integrases - genetics Integron Microbiology Molecular Sequence Data Plasmids - genetics Promoter Pseudomonas aeruginosa Pseudomonas aeruginosa - genetics Spectinomycin Streptomycin Streptomycin - pharmacology |
| title | An integron of class 1 is present on the plasmid pCG4 from Gram-positive bacterium Corynebacterium glutamicum |
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