Protein profile in neuroblastoma cells incubated with S- and R-enantiomers of ibuprofen by iTRAQ-coupled 2-D LC-MS/MS analysis: Possible action of induced proteins on Alzheimer's disease
Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerati...
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| Veröffentlicht in: | Proteomics (Weinheim) 2008-04, Vol.8 (8), p.1595-1607 |
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| creator | Zhang, Jianhua Sui, Jianjun Ching, Chi Bun Chen, Wei Ning |
| description | Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, including Alzheimer's disease (AD). While its prophylactic effect on AD has been suggested, a comprehensive understanding of its mechanism of action remains unclear. Using iTRAQ-coupled 2-D LC-MS/MS analysis, we report here the first study of protein profiles of neuroblastoma cells incubated separately with the two enantiomers of ibuprofen. Three types of cellular proteins, including metabolic enzymes, signaling molecules and cytoskeletal proteins, displayed changes. The changes in the level of a number of enzymes involved in fatty acid synthesis and antioxidant activity in cells incubated with the S-enantiomer were further supported by the real-time PCR analysis as well as the reduced level of reactive oxygen species in cells incubated with the S-enantiomer of ibuprofen. Our findings, therefore, provide the possible mechanism of ibuprofen-induced proteins on AD, and the beneficial effects of ibuprofen in reducing the development of AD. |
| doi_str_mv | 10.1002/pmic.200700556 |
| format | Article |
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It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, including Alzheimer's disease (AD). While its prophylactic effect on AD has been suggested, a comprehensive understanding of its mechanism of action remains unclear. Using iTRAQ-coupled 2-D LC-MS/MS analysis, we report here the first study of protein profiles of neuroblastoma cells incubated separately with the two enantiomers of ibuprofen. Three types of cellular proteins, including metabolic enzymes, signaling molecules and cytoskeletal proteins, displayed changes. The changes in the level of a number of enzymes involved in fatty acid synthesis and antioxidant activity in cells incubated with the S-enantiomer were further supported by the real-time PCR analysis as well as the reduced level of reactive oxygen species in cells incubated with the S-enantiomer of ibuprofen. Our findings, therefore, provide the possible mechanism of ibuprofen-induced proteins on AD, and the beneficial effects of ibuprofen in reducing the development of AD.</description><identifier>ISSN: 1615-9853</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.200700556</identifier><identifier>PMID: 18351690</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>Adult and adolescent clinical studies ; Alzheimer Disease - drug therapy ; Alzheimer Disease - genetics ; Alzheimer Disease - metabolism ; Alzheimer's disease ; Analytical, structural and metabolic biochemistry ; Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Biological and medical sciences ; Biomarkers - analysis ; Biomarkers - metabolism ; Cell Survival - drug effects ; Cells, Cultured ; Cellular protein profile ; Chromatography, Liquid ; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases ; Fundamental and applied biological sciences. Psychology ; Humans ; Ibuprofen ; Ibuprofen - pharmacology ; Indicators and Reagents ; LC/MS-MS analysis ; Mass Spectrometry ; Medical sciences ; Miscellaneous ; Neuroblastoma - drug therapy ; Neuroblastoma - genetics ; Neuroblastoma - metabolism ; Neurology ; Organic mental disorders. Neuropsychology ; Peptide Fragments - analysis ; Proteins ; Proteome - analysis ; Proteome - drug effects ; Proteome - metabolism ; Psychology. Psychoanalysis. Psychiatry ; Psychopathology. Psychiatry ; Reactive Oxygen Species ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; S-enantiomer ; Stereoisomerism ; Tumors of the nervous system. Phacomatoses</subject><ispartof>Proteomics (Weinheim), 2008-04, Vol.8 (8), p.1595-1607</ispartof><rights>Copyright © 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3506-f9211e408d3cd0bcac668f2b5fabfb53abb71099cb8af7d39c5346819165a4763</citedby><cites>FETCH-LOGICAL-c3506-f9211e408d3cd0bcac668f2b5fabfb53abb71099cb8af7d39c5346819165a4763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpmic.200700556$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpmic.200700556$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20297566$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18351690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Jianhua</creatorcontrib><creatorcontrib>Sui, Jianjun</creatorcontrib><creatorcontrib>Ching, Chi Bun</creatorcontrib><creatorcontrib>Chen, Wei Ning</creatorcontrib><title>Protein profile in neuroblastoma cells incubated with S- and R-enantiomers of ibuprofen by iTRAQ-coupled 2-D LC-MS/MS analysis: Possible action of induced proteins on Alzheimer's disease</title><title>Proteomics (Weinheim)</title><addtitle>Proteomics</addtitle><description>Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, including Alzheimer's disease (AD). While its prophylactic effect on AD has been suggested, a comprehensive understanding of its mechanism of action remains unclear. Using iTRAQ-coupled 2-D LC-MS/MS analysis, we report here the first study of protein profiles of neuroblastoma cells incubated separately with the two enantiomers of ibuprofen. Three types of cellular proteins, including metabolic enzymes, signaling molecules and cytoskeletal proteins, displayed changes. The changes in the level of a number of enzymes involved in fatty acid synthesis and antioxidant activity in cells incubated with the S-enantiomer were further supported by the real-time PCR analysis as well as the reduced level of reactive oxygen species in cells incubated with the S-enantiomer of ibuprofen. Our findings, therefore, provide the possible mechanism of ibuprofen-induced proteins on AD, and the beneficial effects of ibuprofen in reducing the development of AD.</description><subject>Adult and adolescent clinical studies</subject><subject>Alzheimer Disease - drug therapy</subject><subject>Alzheimer Disease - genetics</subject><subject>Alzheimer Disease - metabolism</subject><subject>Alzheimer's disease</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Biomarkers - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Cellular protein profile</subject><subject>Chromatography, Liquid</subject><subject>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Ibuprofen</subject><subject>Ibuprofen - pharmacology</subject><subject>Indicators and Reagents</subject><subject>LC/MS-MS analysis</subject><subject>Mass Spectrometry</subject><subject>Medical sciences</subject><subject>Miscellaneous</subject><subject>Neuroblastoma - drug therapy</subject><subject>Neuroblastoma - genetics</subject><subject>Neuroblastoma - metabolism</subject><subject>Neurology</subject><subject>Organic mental disorders. Neuropsychology</subject><subject>Peptide Fragments - analysis</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>Proteome - drug effects</subject><subject>Proteome - metabolism</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopathology. Psychiatry</subject><subject>Reactive Oxygen Species</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>S-enantiomer</subject><subject>Stereoisomerism</subject><subject>Tumors of the nervous system. Phacomatoses</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-T0yAYxjOOO-5avXpULuopXQgBgrdOdf_MtNptd8cjAwRcNCEVklnrR_PTSU2nevPEO8zved4Hnix7geAUQVicb1unpwWEDEJC6KPsDFFEcl5R9Pg4E3yaPY3xK4SIVZw9yU5RhQmiHJ5lv1ah643zYBs66xoD0ujNEDrVyNh3rQTaNE1M13pQsjc1eHD9PdjkQPoarHPjpe9d15oQQWeBU8PeyHigdsDdrmc3ue6GbZN0Rf4eLOb5cnO-3CSxbHbRxXdg1cXoVFosdfLxf0x8Peik2I7RkrEHs-bnvXFpzdsIaheNjOZZdmJlE83zwznJ7i4-3M6v8sWny-v5bJFrTCDNLS8QMiWsaqxrqLTUlFa2UMRKZRXBUimGIOdaVdKyGnNNcEkrxBElsmQUT7I3o2_K830wsReti_tfkd50QxSUI1iyskzgdAR1SI8KxoptcK0MO4Gg2Lcl9m2JY1tJ8PLgPKjW1H_xQz0JeH0AZNSysUF67eKRK2DBGaF7Iz5yD6nC3X_WitXyev5viHzUutibH0etDN8EZZgR8fnjpVjjFbphq4s0TLJXI29lJ-SXkPLcbQqIMIRVlbJU-DfECsjn</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Zhang, Jianhua</creator><creator>Sui, Jianjun</creator><creator>Ching, Chi Bun</creator><creator>Chen, Wei Ning</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Protein profile in neuroblastoma cells incubated with S- and R-enantiomers of ibuprofen by iTRAQ-coupled 2-D LC-MS/MS analysis: Possible action of induced proteins on Alzheimer's disease</title><author>Zhang, Jianhua ; Sui, Jianjun ; Ching, Chi Bun ; Chen, Wei Ning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3506-f9211e408d3cd0bcac668f2b5fabfb53abb71099cb8af7d39c5346819165a4763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult and adolescent clinical studies</topic><topic>Alzheimer Disease - drug therapy</topic><topic>Alzheimer Disease - genetics</topic><topic>Alzheimer Disease - metabolism</topic><topic>Alzheimer's disease</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Biomarkers - metabolism</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Cellular protein profile</topic><topic>Chromatography, Liquid</topic><topic>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Ibuprofen</topic><topic>Ibuprofen - pharmacology</topic><topic>Indicators and Reagents</topic><topic>LC/MS-MS analysis</topic><topic>Mass Spectrometry</topic><topic>Medical sciences</topic><topic>Miscellaneous</topic><topic>Neuroblastoma - drug therapy</topic><topic>Neuroblastoma - genetics</topic><topic>Neuroblastoma - metabolism</topic><topic>Neurology</topic><topic>Organic mental disorders. Neuropsychology</topic><topic>Peptide Fragments - analysis</topic><topic>Proteins</topic><topic>Proteome - analysis</topic><topic>Proteome - drug effects</topic><topic>Proteome - metabolism</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopathology. Psychiatry</topic><topic>Reactive Oxygen Species</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>S-enantiomer</topic><topic>Stereoisomerism</topic><topic>Tumors of the nervous system. Phacomatoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jianhua</creatorcontrib><creatorcontrib>Sui, Jianjun</creatorcontrib><creatorcontrib>Ching, Chi Bun</creatorcontrib><creatorcontrib>Chen, Wei Ning</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jianhua</au><au>Sui, Jianjun</au><au>Ching, Chi Bun</au><au>Chen, Wei Ning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein profile in neuroblastoma cells incubated with S- and R-enantiomers of ibuprofen by iTRAQ-coupled 2-D LC-MS/MS analysis: Possible action of induced proteins on Alzheimer's disease</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2008-04</date><risdate>2008</risdate><volume>8</volume><issue>8</issue><spage>1595</spage><epage>1607</epage><pages>1595-1607</pages><issn>1615-9853</issn><eissn>1615-9861</eissn><abstract>Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, including Alzheimer's disease (AD). While its prophylactic effect on AD has been suggested, a comprehensive understanding of its mechanism of action remains unclear. Using iTRAQ-coupled 2-D LC-MS/MS analysis, we report here the first study of protein profiles of neuroblastoma cells incubated separately with the two enantiomers of ibuprofen. Three types of cellular proteins, including metabolic enzymes, signaling molecules and cytoskeletal proteins, displayed changes. The changes in the level of a number of enzymes involved in fatty acid synthesis and antioxidant activity in cells incubated with the S-enantiomer were further supported by the real-time PCR analysis as well as the reduced level of reactive oxygen species in cells incubated with the S-enantiomer of ibuprofen. Our findings, therefore, provide the possible mechanism of ibuprofen-induced proteins on AD, and the beneficial effects of ibuprofen in reducing the development of AD.</abstract><cop>Weinheim</cop><pub>Wiley-VCH Verlag</pub><pmid>18351690</pmid><doi>10.1002/pmic.200700556</doi><tpages>13</tpages></addata></record> |
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| subjects | Adult and adolescent clinical studies Alzheimer Disease - drug therapy Alzheimer Disease - genetics Alzheimer Disease - metabolism Alzheimer's disease Analytical, structural and metabolic biochemistry Anti-Inflammatory Agents, Non-Steroidal - pharmacology Biological and medical sciences Biomarkers - analysis Biomarkers - metabolism Cell Survival - drug effects Cells, Cultured Cellular protein profile Chromatography, Liquid Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Fundamental and applied biological sciences. Psychology Humans Ibuprofen Ibuprofen - pharmacology Indicators and Reagents LC/MS-MS analysis Mass Spectrometry Medical sciences Miscellaneous Neuroblastoma - drug therapy Neuroblastoma - genetics Neuroblastoma - metabolism Neurology Organic mental disorders. Neuropsychology Peptide Fragments - analysis Proteins Proteome - analysis Proteome - drug effects Proteome - metabolism Psychology. Psychoanalysis. Psychiatry Psychopathology. Psychiatry Reactive Oxygen Species Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism S-enantiomer Stereoisomerism Tumors of the nervous system. Phacomatoses |
| title | Protein profile in neuroblastoma cells incubated with S- and R-enantiomers of ibuprofen by iTRAQ-coupled 2-D LC-MS/MS analysis: Possible action of induced proteins on Alzheimer's disease |
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