The muskrat ( Ondatra zibethicus) as a new reservoir for puumala-like hantavirus strains in Europe
We have used an indirect immunofluorescent assay (IFA) and reverse transcription-PCR (RT-PCR) to screen the sera and tissues of muskrats ( Ondatra zibethica) caught in the northwest of Brandenburg and in the northeast of Saxony-Anhalt, Germany, for hantavirus infection. Kidney and/or lung tissue fro...
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| Veröffentlicht in: | Virus research 1998-10, Vol.57 (2), p.139-150 |
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| creator | Vahlenkamp, Moritz Müller, Thomas Tackmann, Kirsten Löschner, Ulf Schmitz, Herbert Schreiber, Michael |
| description | We have used an indirect immunofluorescent assay (IFA) and reverse transcription-PCR (RT-PCR) to screen the sera and tissues of muskrats (
Ondatra
zibethica) caught in the northwest of Brandenburg and in the northeast of Saxony-Anhalt, Germany, for hantavirus infection. Kidney and/or lung tissue from 6 (3.1%, CI=1.1–6.5%) out of 197 muskrats were found to be positive for genomic sequences of hantavirus by RT-PCR. We could also demonstrate that 14 (5%, CI=2.9–8.7%) out of 266 muskrat’s sera available for testing contained hantavirus-specific antibodies in IFA. Thus, a total of 8% of the investigated muskrat population was found to be positive for hantavirus infection by RT-PCR and IFA. None of the animals was found positive in both tests. Further analysis of the RT-PCR amplified fragments by genomic sequencing revealed sequences mostly related to the puumala (PUU) S segment sequence of the Hällnäs B1 hantavirus strain (97–99% similarity). Our data therefore demonstrate that
Ondatra
zibethicus serves as an additional reservoir for puumala-like hantavirus strains in Europe. The epidemiological implications of this finding for hantavirus infection in Europe and elsewhere are discussed. |
| doi_str_mv | 10.1016/S0168-1702(98)00084-7 |
| format | Article |
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Ondatra
zibethica) caught in the northwest of Brandenburg and in the northeast of Saxony-Anhalt, Germany, for hantavirus infection. Kidney and/or lung tissue from 6 (3.1%, CI=1.1–6.5%) out of 197 muskrats were found to be positive for genomic sequences of hantavirus by RT-PCR. We could also demonstrate that 14 (5%, CI=2.9–8.7%) out of 266 muskrat’s sera available for testing contained hantavirus-specific antibodies in IFA. Thus, a total of 8% of the investigated muskrat population was found to be positive for hantavirus infection by RT-PCR and IFA. None of the animals was found positive in both tests. Further analysis of the RT-PCR amplified fragments by genomic sequencing revealed sequences mostly related to the puumala (PUU) S segment sequence of the Hällnäs B1 hantavirus strain (97–99% similarity). Our data therefore demonstrate that
Ondatra
zibethicus serves as an additional reservoir for puumala-like hantavirus strains in Europe. The epidemiological implications of this finding for hantavirus infection in Europe and elsewhere are discussed.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/S0168-1702(98)00084-7</identifier><identifier>PMID: 9870582</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Viral - blood ; Arvicolinae - virology ; Base Sequence ; Disease Reservoirs ; DNA, Viral - genetics ; Europe - epidemiology ; Fluorescent Antibody Technique, Indirect ; Hantavirus ; Hantavirus - genetics ; Hantavirus - immunology ; Hantavirus - isolation & purification ; Hantavirus Infections - epidemiology ; Hantavirus Infections - transmission ; Hantavirus Infections - veterinary ; Humans ; Kidney - virology ; Lung - virology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; Rodent reservoir ; RT-PCR ; S-segment ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; Serology</subject><ispartof>Virus research, 1998-10, Vol.57 (2), p.139-150</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-a9eb229f796f6aa69a8aba87cf554e265cc70f234a826b861c1350dcf11ca0413</citedby><cites>FETCH-LOGICAL-c391t-a9eb229f796f6aa69a8aba87cf554e265cc70f234a826b861c1350dcf11ca0413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-1702(98)00084-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9870582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vahlenkamp, Moritz</creatorcontrib><creatorcontrib>Müller, Thomas</creatorcontrib><creatorcontrib>Tackmann, Kirsten</creatorcontrib><creatorcontrib>Löschner, Ulf</creatorcontrib><creatorcontrib>Schmitz, Herbert</creatorcontrib><creatorcontrib>Schreiber, Michael</creatorcontrib><title>The muskrat ( Ondatra zibethicus) as a new reservoir for puumala-like hantavirus strains in Europe</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>We have used an indirect immunofluorescent assay (IFA) and reverse transcription-PCR (RT-PCR) to screen the sera and tissues of muskrats (
Ondatra
zibethica) caught in the northwest of Brandenburg and in the northeast of Saxony-Anhalt, Germany, for hantavirus infection. Kidney and/or lung tissue from 6 (3.1%, CI=1.1–6.5%) out of 197 muskrats were found to be positive for genomic sequences of hantavirus by RT-PCR. We could also demonstrate that 14 (5%, CI=2.9–8.7%) out of 266 muskrat’s sera available for testing contained hantavirus-specific antibodies in IFA. Thus, a total of 8% of the investigated muskrat population was found to be positive for hantavirus infection by RT-PCR and IFA. None of the animals was found positive in both tests. Further analysis of the RT-PCR amplified fragments by genomic sequencing revealed sequences mostly related to the puumala (PUU) S segment sequence of the Hällnäs B1 hantavirus strain (97–99% similarity). Our data therefore demonstrate that
Ondatra
zibethicus serves as an additional reservoir for puumala-like hantavirus strains in Europe. The epidemiological implications of this finding for hantavirus infection in Europe and elsewhere are discussed.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Arvicolinae - virology</subject><subject>Base Sequence</subject><subject>Disease Reservoirs</subject><subject>DNA, Viral - genetics</subject><subject>Europe - epidemiology</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Hantavirus</subject><subject>Hantavirus - genetics</subject><subject>Hantavirus - immunology</subject><subject>Hantavirus - isolation & purification</subject><subject>Hantavirus Infections - epidemiology</subject><subject>Hantavirus Infections - transmission</subject><subject>Hantavirus Infections - veterinary</subject><subject>Humans</subject><subject>Kidney - virology</subject><subject>Lung - virology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>Rodent reservoir</subject><subject>RT-PCR</subject><subject>S-segment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Serology</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUlLBDEQhYMoOi4_QchJ9NCapLuznEQGNxA8qOdQna5mor2MSWdEf72tM3j1UnV436uC9wg55uycMy4vnqahM66YODX6jDGmi0xtkRnXSmSqMGKbzP6QPbIf4-sEyVzJXbJrtGKlFjNSPS-Qdim-BRjpKX3saxgD0C9f4bjwLsUzCpEC7fGDBowYVoMPtBkCXabUQQtZ69-QLqAfYeVDijROft9H6nt6ncKwxEOy00Ab8WizD8jLzfXz_C57eLy9n189ZC43fMzAYCWEaZSRjQSQBjRUoJVryrJAIUvnFGtEXoAWstKSO56XrHYN5w5YwfMDcrK-uwzDe8I42s5Hh20LPQ4pWmk4K6SS_4JclpyJkk1guQZdGGIM2Nhl8B2ET8uZ_SnB_pZgfxK2RtvfEqyafMebB6nqsP5zbVKf9Mu1jlMcK4_BRuexd1j7gG609eD_-fANXn2W2w</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Vahlenkamp, Moritz</creator><creator>Müller, Thomas</creator><creator>Tackmann, Kirsten</creator><creator>Löschner, Ulf</creator><creator>Schmitz, Herbert</creator><creator>Schreiber, Michael</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19981001</creationdate><title>The muskrat ( Ondatra zibethicus) as a new reservoir for puumala-like hantavirus strains in Europe</title><author>Vahlenkamp, Moritz ; Müller, Thomas ; Tackmann, Kirsten ; Löschner, Ulf ; Schmitz, Herbert ; Schreiber, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-a9eb229f796f6aa69a8aba87cf554e265cc70f234a826b861c1350dcf11ca0413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Arvicolinae - virology</topic><topic>Base Sequence</topic><topic>Disease Reservoirs</topic><topic>DNA, Viral - genetics</topic><topic>Europe - epidemiology</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Hantavirus</topic><topic>Hantavirus - genetics</topic><topic>Hantavirus - immunology</topic><topic>Hantavirus - isolation & purification</topic><topic>Hantavirus Infections - epidemiology</topic><topic>Hantavirus Infections - transmission</topic><topic>Hantavirus Infections - veterinary</topic><topic>Humans</topic><topic>Kidney - virology</topic><topic>Lung - virology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>Rodent reservoir</topic><topic>RT-PCR</topic><topic>S-segment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Serology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vahlenkamp, Moritz</creatorcontrib><creatorcontrib>Müller, Thomas</creatorcontrib><creatorcontrib>Tackmann, Kirsten</creatorcontrib><creatorcontrib>Löschner, Ulf</creatorcontrib><creatorcontrib>Schmitz, Herbert</creatorcontrib><creatorcontrib>Schreiber, Michael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vahlenkamp, Moritz</au><au>Müller, Thomas</au><au>Tackmann, Kirsten</au><au>Löschner, Ulf</au><au>Schmitz, Herbert</au><au>Schreiber, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The muskrat ( Ondatra zibethicus) as a new reservoir for puumala-like hantavirus strains in Europe</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>57</volume><issue>2</issue><spage>139</spage><epage>150</epage><pages>139-150</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>We have used an indirect immunofluorescent assay (IFA) and reverse transcription-PCR (RT-PCR) to screen the sera and tissues of muskrats (
Ondatra
zibethica) caught in the northwest of Brandenburg and in the northeast of Saxony-Anhalt, Germany, for hantavirus infection. Kidney and/or lung tissue from 6 (3.1%, CI=1.1–6.5%) out of 197 muskrats were found to be positive for genomic sequences of hantavirus by RT-PCR. We could also demonstrate that 14 (5%, CI=2.9–8.7%) out of 266 muskrat’s sera available for testing contained hantavirus-specific antibodies in IFA. Thus, a total of 8% of the investigated muskrat population was found to be positive for hantavirus infection by RT-PCR and IFA. None of the animals was found positive in both tests. Further analysis of the RT-PCR amplified fragments by genomic sequencing revealed sequences mostly related to the puumala (PUU) S segment sequence of the Hällnäs B1 hantavirus strain (97–99% similarity). Our data therefore demonstrate that
Ondatra
zibethicus serves as an additional reservoir for puumala-like hantavirus strains in Europe. The epidemiological implications of this finding for hantavirus infection in Europe and elsewhere are discussed.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9870582</pmid><doi>10.1016/S0168-1702(98)00084-7</doi><tpages>12</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Animals Antibodies, Viral - blood Arvicolinae - virology Base Sequence Disease Reservoirs DNA, Viral - genetics Europe - epidemiology Fluorescent Antibody Technique, Indirect Hantavirus Hantavirus - genetics Hantavirus - immunology Hantavirus - isolation & purification Hantavirus Infections - epidemiology Hantavirus Infections - transmission Hantavirus Infections - veterinary Humans Kidney - virology Lung - virology Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics RNA, Viral - isolation & purification Rodent reservoir RT-PCR S-segment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Serology |
| title | The muskrat ( Ondatra zibethicus) as a new reservoir for puumala-like hantavirus strains in Europe |
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