Dual Microsporidial Infection Due to Vittaforma corneae and Encephalitozoon hellem in a Patient with AIDS
A 46-year-old human immunodeficiency virus-infected Swiss citizen living in Tanzania presented with respiratory, abdominal, and urogenital complaints. Microsporidial spores were isolated from urine and a sinunasal aspirate and were propagated in MRC-5 cell cultures. Western blot analysis and ribopri...
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| Veröffentlicht in: | Clinical infectious diseases 1998-12, Vol.27 (6), p.1521-1524 |
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| creator | Deplazes, P. Mathis, A. van Saanen, M. Iten, A. Keller, R. Tanner, I. Glauser, M. P. Weber, R. Canning, E. U. |
| description | A 46-year-old human immunodeficiency virus-infected Swiss citizen living in Tanzania presented with respiratory, abdominal, and urogenital complaints. Microsporidial spores were isolated from urine and a sinunasal aspirate and were propagated in MRC-5 cell cultures. Western blot analysis and riboprinting identified the sinunasal isolate as Encephalitozoon hellem. Electron microscopic investigation of the urine isolate revealed spores with diplokaryotic nuclei and five to six isofilar coils of the polar tube and sporonts with two or three diplokarya. All stages were enveloped by two membranes, corresponding to a cisterna of host endoplasmic reticulum studded with ribosomes. These characteristics have been described for the genus Vittaforma. Western blot analysis of this isolate revealed a banding pattern identical to that of the Vittaforma corneae reference isolate. Part of the small subunit rRNA gene was amplified, sequenced (239 base pairs), and found to be identical to that of V. corneae. This is the second isolation of V. corneae and the first description of urinary tract infection due to V. corneae in a patient with AIDS. |
| doi_str_mv | 10.1086/515023 |
| format | Article |
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P. ; Weber, R. ; Canning, E. U.</creator><creatorcontrib>Deplazes, P. ; Mathis, A. ; van Saanen, M. ; Iten, A. ; Keller, R. ; Tanner, I. ; Glauser, M. P. ; Weber, R. ; Canning, E. U.</creatorcontrib><description>A 46-year-old human immunodeficiency virus-infected Swiss citizen living in Tanzania presented with respiratory, abdominal, and urogenital complaints. Microsporidial spores were isolated from urine and a sinunasal aspirate and were propagated in MRC-5 cell cultures. Western blot analysis and riboprinting identified the sinunasal isolate as Encephalitozoon hellem. Electron microscopic investigation of the urine isolate revealed spores with diplokaryotic nuclei and five to six isofilar coils of the polar tube and sporonts with two or three diplokarya. All stages were enveloped by two membranes, corresponding to a cisterna of host endoplasmic reticulum studded with ribosomes. These characteristics have been described for the genus Vittaforma. Western blot analysis of this isolate revealed a banding pattern identical to that of the Vittaforma corneae reference isolate. Part of the small subunit rRNA gene was amplified, sequenced (239 base pairs), and found to be identical to that of V. corneae. This is the second isolation of V. corneae and the first description of urinary tract infection due to V. corneae in a patient with AIDS.</description><identifier>ISSN: 1058-4838</identifier><identifier>EISSN: 1537-6591</identifier><identifier>DOI: 10.1086/515023</identifier><identifier>PMID: 9868671</identifier><identifier>CODEN: CIDIEL</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>AIDS ; AIDS-Related Opportunistic Infections - parasitology ; AIDS/HIV ; Animals ; Biological and medical sciences ; Blotting, Western ; Encephalitozoon ; Encephalitozoon - isolation & purification ; Encephalitozoon hellem ; Encephalitozoonosis - complications ; Endoplasmic reticulum ; Human immunodeficiency virus 1 ; Human viral diseases ; Humans ; Infections ; Infectious diseases ; Male ; Medical sciences ; Mice ; Mice, Nude - parasitology ; Microsporida - isolation & purification ; Microsporida - ultrastructure ; Microsporidia ; Middle Aged ; Protozoan Infections - parasitology ; Sinuses ; Spores ; Tropical medicine ; Urine ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Vittaforma ; Vittaforma corneae</subject><ispartof>Clinical infectious diseases, 1998-12, Vol.27 (6), p.1521-1524</ispartof><rights>Copyright 1998 The Infectious Diseases Society of America</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-60ea34a3930b3ebb994ff8ee999d1999189110621008fc1fbd429d869aacfe3b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4481760$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4481760$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1615330$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9868671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deplazes, P.</creatorcontrib><creatorcontrib>Mathis, A.</creatorcontrib><creatorcontrib>van Saanen, M.</creatorcontrib><creatorcontrib>Iten, A.</creatorcontrib><creatorcontrib>Keller, R.</creatorcontrib><creatorcontrib>Tanner, I.</creatorcontrib><creatorcontrib>Glauser, M. P.</creatorcontrib><creatorcontrib>Weber, R.</creatorcontrib><creatorcontrib>Canning, E. U.</creatorcontrib><title>Dual Microsporidial Infection Due to Vittaforma corneae and Encephalitozoon hellem in a Patient with AIDS</title><title>Clinical infectious diseases</title><addtitle>Clinical Infectious Diseases</addtitle><description>A 46-year-old human immunodeficiency virus-infected Swiss citizen living in Tanzania presented with respiratory, abdominal, and urogenital complaints. Microsporidial spores were isolated from urine and a sinunasal aspirate and were propagated in MRC-5 cell cultures. Western blot analysis and riboprinting identified the sinunasal isolate as Encephalitozoon hellem. Electron microscopic investigation of the urine isolate revealed spores with diplokaryotic nuclei and five to six isofilar coils of the polar tube and sporonts with two or three diplokarya. All stages were enveloped by two membranes, corresponding to a cisterna of host endoplasmic reticulum studded with ribosomes. These characteristics have been described for the genus Vittaforma. Western blot analysis of this isolate revealed a banding pattern identical to that of the Vittaforma corneae reference isolate. Part of the small subunit rRNA gene was amplified, sequenced (239 base pairs), and found to be identical to that of V. corneae. This is the second isolation of V. corneae and the first description of urinary tract infection due to V. corneae in a patient with AIDS.</description><subject>AIDS</subject><subject>AIDS-Related Opportunistic Infections - parasitology</subject><subject>AIDS/HIV</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Encephalitozoon</subject><subject>Encephalitozoon - isolation & purification</subject><subject>Encephalitozoon hellem</subject><subject>Encephalitozoonosis - complications</subject><subject>Endoplasmic reticulum</subject><subject>Human immunodeficiency virus 1</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude - parasitology</subject><subject>Microsporida - isolation & purification</subject><subject>Microsporida - ultrastructure</subject><subject>Microsporidia</subject><subject>Middle Aged</subject><subject>Protozoan Infections - parasitology</subject><subject>Sinuses</subject><subject>Spores</subject><subject>Tropical medicine</subject><subject>Urine</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Vittaforma</subject><subject>Vittaforma corneae</subject><issn>1058-4838</issn><issn>1537-6591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhgex1LbqL1DIhfRu2mQy-bos3WpXWixUi3gTMpkTNnVmsiYZ_Pj1Ztlle9mb5ITn4eXwpqreEnxGsOTnjDDc0BfVEWFU1Jwp8rLMmMm6lVS-qo5TesSYEInZYXWoJJdckKPKL2YzoFtvY0jrEH3vy3M5ObDZhwktZkA5oAefs3EhjgbZECcwgMzUo6vJwnplBp_Dv1DsFQwDjMhPyKA7kz1MGf32eYUulov719WBM0OCN7v7pPr28err5XV98-XT8vLiprZU0VxzDIa2psy4o9B1SrXOSQClVE_KQaQiBPOGYCydJa7r20b1kitjrAPa0ZPqdJu7juHXDCnr0SdbNjMThDlprrCSgpFnRSKaphGMPYmbklIEp9fRjyb-1QTrTfl6W34R3-8S526Efq_t2i78w46bZM3gopmsT09pvPwdxUV7t9UeUw5xj9tWEsE3uN5inzL82WMTf2ouqGD6-vsPfS8fhLj9fKcV_Q-OU6L8</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Deplazes, P.</creator><creator>Mathis, A.</creator><creator>van Saanen, M.</creator><creator>Iten, A.</creator><creator>Keller, R.</creator><creator>Tanner, I.</creator><creator>Glauser, M. P.</creator><creator>Weber, R.</creator><creator>Canning, E. U.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Dual Microsporidial Infection Due to Vittaforma corneae and Encephalitozoon hellem in a Patient with AIDS</title><author>Deplazes, P. ; Mathis, A. ; van Saanen, M. ; Iten, A. ; Keller, R. ; Tanner, I. ; Glauser, M. P. ; Weber, R. ; Canning, E. U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-60ea34a3930b3ebb994ff8ee999d1999189110621008fc1fbd429d869aacfe3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>AIDS</topic><topic>AIDS-Related Opportunistic Infections - parasitology</topic><topic>AIDS/HIV</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Encephalitozoon</topic><topic>Encephalitozoon - isolation & purification</topic><topic>Encephalitozoon hellem</topic><topic>Encephalitozoonosis - complications</topic><topic>Endoplasmic reticulum</topic><topic>Human immunodeficiency virus 1</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Nude - parasitology</topic><topic>Microsporida - isolation & purification</topic><topic>Microsporida - ultrastructure</topic><topic>Microsporidia</topic><topic>Middle Aged</topic><topic>Protozoan Infections - parasitology</topic><topic>Sinuses</topic><topic>Spores</topic><topic>Tropical medicine</topic><topic>Urine</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Vittaforma</topic><topic>Vittaforma corneae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deplazes, P.</creatorcontrib><creatorcontrib>Mathis, A.</creatorcontrib><creatorcontrib>van Saanen, M.</creatorcontrib><creatorcontrib>Iten, A.</creatorcontrib><creatorcontrib>Keller, R.</creatorcontrib><creatorcontrib>Tanner, I.</creatorcontrib><creatorcontrib>Glauser, M. P.</creatorcontrib><creatorcontrib>Weber, R.</creatorcontrib><creatorcontrib>Canning, E. U.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deplazes, P.</au><au>Mathis, A.</au><au>van Saanen, M.</au><au>Iten, A.</au><au>Keller, R.</au><au>Tanner, I.</au><au>Glauser, M. P.</au><au>Weber, R.</au><au>Canning, E. U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual Microsporidial Infection Due to Vittaforma corneae and Encephalitozoon hellem in a Patient with AIDS</atitle><jtitle>Clinical infectious diseases</jtitle><addtitle>Clinical Infectious Diseases</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>27</volume><issue>6</issue><spage>1521</spage><epage>1524</epage><pages>1521-1524</pages><issn>1058-4838</issn><eissn>1537-6591</eissn><coden>CIDIEL</coden><abstract>A 46-year-old human immunodeficiency virus-infected Swiss citizen living in Tanzania presented with respiratory, abdominal, and urogenital complaints. Microsporidial spores were isolated from urine and a sinunasal aspirate and were propagated in MRC-5 cell cultures. Western blot analysis and riboprinting identified the sinunasal isolate as Encephalitozoon hellem. Electron microscopic investigation of the urine isolate revealed spores with diplokaryotic nuclei and five to six isofilar coils of the polar tube and sporonts with two or three diplokarya. All stages were enveloped by two membranes, corresponding to a cisterna of host endoplasmic reticulum studded with ribosomes. These characteristics have been described for the genus Vittaforma. Western blot analysis of this isolate revealed a banding pattern identical to that of the Vittaforma corneae reference isolate. Part of the small subunit rRNA gene was amplified, sequenced (239 base pairs), and found to be identical to that of V. corneae. This is the second isolation of V. corneae and the first description of urinary tract infection due to V. corneae in a patient with AIDS.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>9868671</pmid><doi>10.1086/515023</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical infectious diseases, 1998-12, Vol.27 (6), p.1521-1524 |
| issn | 1058-4838 1537-6591 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; EZB Electronic Journals Library; Oxford Journals |
| subjects | AIDS AIDS-Related Opportunistic Infections - parasitology AIDS/HIV Animals Biological and medical sciences Blotting, Western Encephalitozoon Encephalitozoon - isolation & purification Encephalitozoon hellem Encephalitozoonosis - complications Endoplasmic reticulum Human immunodeficiency virus 1 Human viral diseases Humans Infections Infectious diseases Male Medical sciences Mice Mice, Nude - parasitology Microsporida - isolation & purification Microsporida - ultrastructure Microsporidia Middle Aged Protozoan Infections - parasitology Sinuses Spores Tropical medicine Urine Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Vittaforma Vittaforma corneae |
| title | Dual Microsporidial Infection Due to Vittaforma corneae and Encephalitozoon hellem in a Patient with AIDS |
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