Structure and polymorphism of the Chironomus thummi gene encoding special lobe-specific silk protein, ssp160

cDNA encoding Chironomus thummi ssp160 was used to isolate a genomic clone that hybridized in situ to band A2b on polytene chromosome IV, the site of the ssp160 gene. DNA sequencing, primer extension and gene/cDNA nucleotide sequence alignment revealed the gene contains six exons and five introns; 7...

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Veröffentlicht in:	Gene 1998-11, Vol.223 (1-2), p.347-354
Hauptverfasser:	Berezikov, Eugene, Blinov, Alexander G, Scherbik, Svetlana, Cox, Carol K, Case, Steven T
Format:	Artikel
Sprache:	eng
Schlagworte:	alleles Amino Acid Sequence amino acid sequences animal proteins Animals Base Sequence Chironomidae Chironomidae - genetics Chironomidae - growth & development Chironomus riparius Chironomus thummi Cloning, Molecular codons complementary DNA deletions Evolution, Molecular Exons genbank/af036895 geographical variation Glycoproteins - genetics In-situ hybridization Insect Proteins Introns Larva Molecular Sequence Data multigene family nucleic acid hybridization Nucleotide sequence variants nucleotide sequences Polymorphism, Genetic Polymorphism, Restriction Fragment Length polytene chromosomes population promoter regions Repeated glycosylation motifs Repetitive Sequences, Nucleic Acid Restriction fragment length polymorphism salivary glands Salivary Proteins and Peptides - genetics Sequence Alignment Sequence Analysis, DNA Silk structural genes Transcription start point
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