The Yeast Mitochondrial Intermembrane Space: Purification and Analysis of Two Distinct Fractions

We have developed a protocol for the sequential release of the intermembrane space (IMS) content ofSaccharomyces cerevisiaemitochondria. Two distinct fractions were obtained: a soluble IMS with cytochromeb2as key marker and a salt-extractable IMS with cytochromecas key marker. The identity of severa...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Analytical biochemistry 1998-12, Vol.265 (1), p.123-128
Hauptverfasser:	Martin, Heiko, Eckerskorn, Christoph, Gärtner, Frank, Rassow, Joachim, Lottspeich, Fritz, Pfanner, Nikolaus
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Fractionation Fungal Proteins - isolation & purification Fungal Proteins - metabolism Intracellular Membranes - ultrastructure Mitochondria - metabolism Saccharomyces cerevisiae - ultrastructure Ultracentrifugation
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!