A Gel Diffusion Assay for Quantification of Pectin Methylesterase Activity

Increased binding of ruthenium red to pectin as the number of methyl esters attached to the pectin decreases was used as the basis for a gel diffusion assay for pectin methylesterase (PME, EC 3.1.1.11) activity. The stained zone diameters resulting from the hydrolysis of 0.1% (w/v) 90% esterified pe...

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Veröffentlicht in:	Analytical biochemistry 1998-11, Vol.264 (2), p.149-157
Hauptverfasser:	Downie, Bruce, Dirk, Lynnette M.A., Hadfield, Kristen A., Wilkins, Thea A., Bennett, Alan B., Bradford, Kent J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Carboxylic Ester Hydrolases - metabolism Cell Extracts - chemistry Diffusion Electrophoresis, Agar Gel - methods Esterification Fruit Hydrogen-Ion Concentration Hydrolysis Magnoliopsida - embryology Magnoliopsida - enzymology Pectins - metabolism Polygalacturonase - metabolism Polysaccharide-Lyases - metabolism Ruthenium Red - metabolism Seeds - enzymology Sensitivity and Specificity Sodium Hydroxide - pharmacology Staining and Labeling - methods Time Factors
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