Expression of cyclooxygenases in ductus arteriosus of fetal and newborn pigs
Objective: We studied the ontogeny of the expression of cyclooxygenase-1 and cyclooxygenase-2 in the ductus arteriosus and evaluated their functional significance. Study Design: The expression of cyclooxygenase-1 and cyclooxygenase-2 was studied in ductus arteriosus of fetal (at ~75% gestation) and...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1998-12, Vol.179 (6), p.1618-1626 |
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| container_title | American journal of obstetrics and gynecology |
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| creator | Guerguerian, Anne-Marie Hardy, Pierre Bhattacharya, Mousumi Olley, Peter Clyman, Ronald I. Fouron, Jean-Claude Chemtob, Sylvain |
| description | Objective: We studied the ontogeny of the expression of cyclooxygenase-1 and cyclooxygenase-2 in the ductus arteriosus and evaluated their functional significance.
Study Design: The expression of cyclooxygenase-1 and cyclooxygenase-2 was studied in ductus arteriosus of fetal (at ~75% gestation) and term newborn pig. Effects of selective inhibitors of cyclooxygenase-1 and cyclooxygenase-2 on ductal patency were evaluated by Doppler ultrasonography.
Results: Ductus arteriosus of the fetus expressed virtually only cyclooxygenase-1 immunoreactive protein and activity. In contrast, the ductus of the term newborn pig (90% of prostaglandin E
2 formation. The selective cyclooxygenase-2 inhibitor DuP697 reduced prostaglandin E
2 levels in the ductus arteriosus, albeit not in plasma, but did not affect ductal patency in the newborn pig ( |
| doi_str_mv | 10.1016/S0002-9378(98)70035-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69085695</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0002937898700353</els_id><sourcerecordid>69085695</sourcerecordid><originalsourceid>FETCH-LOGICAL-c484t-1b32c9fbb8b8492b820d48668d3310935a9f519720cab2f72cfd58027420f4793</originalsourceid><addsrcrecordid>eNqFkLtO7DAQhl2AuD8CUgqEoAg4duzY1RFC3KSVKIDacpwxMsraiyc5sG9Pll1BSTW3f_4ZfYQcV_SiopW8fKKUslLzRp1pdd5QykXJt8jeT3uX7CO-rUqm2Q7Z0UoISZs9Mrv5XGRADCkWyRdu6fqUPpevEC0CFiEW3eiGEQubB8gh4ZROOg-D7QsbuyLCR5tyLBbhFQ_Jtrc9wtEmHpCX25vn6_ty9nj3cH01K12t6qGsWs6c9m2rWlVr1ipGu1pJqTrOK6q5sNqLSjeMOtsy3zDnO6Eoa2pGfd1ofkBO176LnN5HwMHMAzroexshjWikpkpILSahWAtdTogZvFnkMLd5aSpqVuTMNzmzQmS0Mt_kDJ_2jjcHxnYO3c_WBts0P9nMLTrb-2yjC_hrLrmUcmXzby2DCcb_ANmgCxAddCGDG0yXwh-PfAHWWowV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69085695</pqid></control><display><type>article</type><title>Expression of cyclooxygenases in ductus arteriosus of fetal and newborn pigs</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Guerguerian, Anne-Marie ; Hardy, Pierre ; Bhattacharya, Mousumi ; Olley, Peter ; Clyman, Ronald I. ; Fouron, Jean-Claude ; Chemtob, Sylvain</creator><creatorcontrib>Guerguerian, Anne-Marie ; Hardy, Pierre ; Bhattacharya, Mousumi ; Olley, Peter ; Clyman, Ronald I. ; Fouron, Jean-Claude ; Chemtob, Sylvain</creatorcontrib><description>Objective: We studied the ontogeny of the expression of cyclooxygenase-1 and cyclooxygenase-2 in the ductus arteriosus and evaluated their functional significance.
Study Design: The expression of cyclooxygenase-1 and cyclooxygenase-2 was studied in ductus arteriosus of fetal (at ~75% gestation) and term newborn pig. Effects of selective inhibitors of cyclooxygenase-1 and cyclooxygenase-2 on ductal patency were evaluated by Doppler ultrasonography.
Results: Ductus arteriosus of the fetus expressed virtually only cyclooxygenase-1 immunoreactive protein and activity. In contrast, the ductus of the term newborn pig (<45 minutes old) contained proteins of both cyclooxygenase-1 and cyclooxygenase-2, but the latter contributed to >90% of prostaglandin E
2 formation. The selective cyclooxygenase-2 inhibitor DuP697 reduced prostaglandin E
2 levels in the ductus arteriosus, albeit not in plasma, but did not affect ductal patency in the newborn pig (<1
½ hours old); in contrast, the cyclooxygenase-1 inhibitor valeryl salicylate, like indomethacin, markedly reduced levels of prostaglandin E
2 in the plasma and ductus arteriosus and caused significant constriction of the ductus arteriosus.
Conclusion: The ductus arteriosus of the term newborn pig, in contrast to that of the fetus, expresses cyclooxygenase-2, but circulating prostaglandins, arising mostly from cyclooxygenase-1, seem to exert the major control on ductal patency in vivo. Our data suggest that cyclooxygenase-2 inhibitors might be better alternatives for the fetus than nonselective cyclooxygenase blockers if indicated for maternal conditions such as inflammation or for tocolysis. (Am J Obstet Gynecol 1998;179:1618-26.)</description><identifier>ISSN: 0002-9378</identifier><identifier>DOI: 10.1016/S0002-9378(98)70035-3</identifier><identifier>PMID: 9855607</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>Philadelphia, PA: Mosby, Inc</publisher><subject>Animals ; Animals, Newborn - metabolism ; Biological and medical sciences ; cyclooxygenase ; Cyclooxygenase 1 ; Cyclooxygenase 2 ; Dinoprostone - biosynthesis ; Ductus arteriosus ; Ductus Arteriosus - drug effects ; Ductus Arteriosus - enzymology ; Ductus Arteriosus - metabolism ; Enzyme Inhibitors - pharmacology ; fetus ; Fetus - blood supply ; Fetus - enzymology ; Fundamental and applied biological sciences. Psychology ; Isoenzymes - metabolism ; Mother. Fetoplacental unit. Mammary gland. Milk ; newborn ; Pregnancy. Parturition. Lactation ; Prostaglandin-Endoperoxide Synthases - metabolism ; prostaglandins ; Swine ; Vertebrates: reproduction</subject><ispartof>American journal of obstetrics and gynecology, 1998-12, Vol.179 (6), p.1618-1626</ispartof><rights>1998 Mosby, Inc.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-1b32c9fbb8b8492b820d48668d3310935a9f519720cab2f72cfd58027420f4793</citedby><cites>FETCH-LOGICAL-c484t-1b32c9fbb8b8492b820d48668d3310935a9f519720cab2f72cfd58027420f4793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0002937898700353$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1636663$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9855607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guerguerian, Anne-Marie</creatorcontrib><creatorcontrib>Hardy, Pierre</creatorcontrib><creatorcontrib>Bhattacharya, Mousumi</creatorcontrib><creatorcontrib>Olley, Peter</creatorcontrib><creatorcontrib>Clyman, Ronald I.</creatorcontrib><creatorcontrib>Fouron, Jean-Claude</creatorcontrib><creatorcontrib>Chemtob, Sylvain</creatorcontrib><title>Expression of cyclooxygenases in ductus arteriosus of fetal and newborn pigs</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>Objective: We studied the ontogeny of the expression of cyclooxygenase-1 and cyclooxygenase-2 in the ductus arteriosus and evaluated their functional significance.
Study Design: The expression of cyclooxygenase-1 and cyclooxygenase-2 was studied in ductus arteriosus of fetal (at ~75% gestation) and term newborn pig. Effects of selective inhibitors of cyclooxygenase-1 and cyclooxygenase-2 on ductal patency were evaluated by Doppler ultrasonography.
Results: Ductus arteriosus of the fetus expressed virtually only cyclooxygenase-1 immunoreactive protein and activity. In contrast, the ductus of the term newborn pig (<45 minutes old) contained proteins of both cyclooxygenase-1 and cyclooxygenase-2, but the latter contributed to >90% of prostaglandin E
2 formation. The selective cyclooxygenase-2 inhibitor DuP697 reduced prostaglandin E
2 levels in the ductus arteriosus, albeit not in plasma, but did not affect ductal patency in the newborn pig (<1
½ hours old); in contrast, the cyclooxygenase-1 inhibitor valeryl salicylate, like indomethacin, markedly reduced levels of prostaglandin E
2 in the plasma and ductus arteriosus and caused significant constriction of the ductus arteriosus.
Conclusion: The ductus arteriosus of the term newborn pig, in contrast to that of the fetus, expresses cyclooxygenase-2, but circulating prostaglandins, arising mostly from cyclooxygenase-1, seem to exert the major control on ductal patency in vivo. Our data suggest that cyclooxygenase-2 inhibitors might be better alternatives for the fetus than nonselective cyclooxygenase blockers if indicated for maternal conditions such as inflammation or for tocolysis. (Am J Obstet Gynecol 1998;179:1618-26.)</description><subject>Animals</subject><subject>Animals, Newborn - metabolism</subject><subject>Biological and medical sciences</subject><subject>cyclooxygenase</subject><subject>Cyclooxygenase 1</subject><subject>Cyclooxygenase 2</subject><subject>Dinoprostone - biosynthesis</subject><subject>Ductus arteriosus</subject><subject>Ductus Arteriosus - drug effects</subject><subject>Ductus Arteriosus - enzymology</subject><subject>Ductus Arteriosus - metabolism</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>fetus</subject><subject>Fetus - blood supply</subject><subject>Fetus - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isoenzymes - metabolism</subject><subject>Mother. Fetoplacental unit. Mammary gland. Milk</subject><subject>newborn</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Prostaglandin-Endoperoxide Synthases - metabolism</subject><subject>prostaglandins</subject><subject>Swine</subject><subject>Vertebrates: reproduction</subject><issn>0002-9378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtO7DAQhl2AuD8CUgqEoAg4duzY1RFC3KSVKIDacpwxMsraiyc5sG9Pll1BSTW3f_4ZfYQcV_SiopW8fKKUslLzRp1pdd5QykXJt8jeT3uX7CO-rUqm2Q7Z0UoISZs9Mrv5XGRADCkWyRdu6fqUPpevEC0CFiEW3eiGEQubB8gh4ZROOg-D7QsbuyLCR5tyLBbhFQ_Jtrc9wtEmHpCX25vn6_ty9nj3cH01K12t6qGsWs6c9m2rWlVr1ipGu1pJqTrOK6q5sNqLSjeMOtsy3zDnO6Eoa2pGfd1ofkBO176LnN5HwMHMAzroexshjWikpkpILSahWAtdTogZvFnkMLd5aSpqVuTMNzmzQmS0Mt_kDJ_2jjcHxnYO3c_WBts0P9nMLTrb-2yjC_hrLrmUcmXzby2DCcb_ANmgCxAddCGDG0yXwh-PfAHWWowV</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Guerguerian, Anne-Marie</creator><creator>Hardy, Pierre</creator><creator>Bhattacharya, Mousumi</creator><creator>Olley, Peter</creator><creator>Clyman, Ronald I.</creator><creator>Fouron, Jean-Claude</creator><creator>Chemtob, Sylvain</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Expression of cyclooxygenases in ductus arteriosus of fetal and newborn pigs</title><author>Guerguerian, Anne-Marie ; Hardy, Pierre ; Bhattacharya, Mousumi ; Olley, Peter ; Clyman, Ronald I. ; Fouron, Jean-Claude ; Chemtob, Sylvain</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-1b32c9fbb8b8492b820d48668d3310935a9f519720cab2f72cfd58027420f4793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Animals, Newborn - metabolism</topic><topic>Biological and medical sciences</topic><topic>cyclooxygenase</topic><topic>Cyclooxygenase 1</topic><topic>Cyclooxygenase 2</topic><topic>Dinoprostone - biosynthesis</topic><topic>Ductus arteriosus</topic><topic>Ductus Arteriosus - drug effects</topic><topic>Ductus Arteriosus - enzymology</topic><topic>Ductus Arteriosus - metabolism</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>fetus</topic><topic>Fetus - blood supply</topic><topic>Fetus - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isoenzymes - metabolism</topic><topic>Mother. Fetoplacental unit. Mammary gland. Milk</topic><topic>newborn</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Prostaglandin-Endoperoxide Synthases - metabolism</topic><topic>prostaglandins</topic><topic>Swine</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guerguerian, Anne-Marie</creatorcontrib><creatorcontrib>Hardy, Pierre</creatorcontrib><creatorcontrib>Bhattacharya, Mousumi</creatorcontrib><creatorcontrib>Olley, Peter</creatorcontrib><creatorcontrib>Clyman, Ronald I.</creatorcontrib><creatorcontrib>Fouron, Jean-Claude</creatorcontrib><creatorcontrib>Chemtob, Sylvain</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guerguerian, Anne-Marie</au><au>Hardy, Pierre</au><au>Bhattacharya, Mousumi</au><au>Olley, Peter</au><au>Clyman, Ronald I.</au><au>Fouron, Jean-Claude</au><au>Chemtob, Sylvain</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of cyclooxygenases in ductus arteriosus of fetal and newborn pigs</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>179</volume><issue>6</issue><spage>1618</spage><epage>1626</epage><pages>1618-1626</pages><issn>0002-9378</issn><coden>AJOGAH</coden><abstract>Objective: We studied the ontogeny of the expression of cyclooxygenase-1 and cyclooxygenase-2 in the ductus arteriosus and evaluated their functional significance.
Study Design: The expression of cyclooxygenase-1 and cyclooxygenase-2 was studied in ductus arteriosus of fetal (at ~75% gestation) and term newborn pig. Effects of selective inhibitors of cyclooxygenase-1 and cyclooxygenase-2 on ductal patency were evaluated by Doppler ultrasonography.
Results: Ductus arteriosus of the fetus expressed virtually only cyclooxygenase-1 immunoreactive protein and activity. In contrast, the ductus of the term newborn pig (<45 minutes old) contained proteins of both cyclooxygenase-1 and cyclooxygenase-2, but the latter contributed to >90% of prostaglandin E
2 formation. The selective cyclooxygenase-2 inhibitor DuP697 reduced prostaglandin E
2 levels in the ductus arteriosus, albeit not in plasma, but did not affect ductal patency in the newborn pig (<1
½ hours old); in contrast, the cyclooxygenase-1 inhibitor valeryl salicylate, like indomethacin, markedly reduced levels of prostaglandin E
2 in the plasma and ductus arteriosus and caused significant constriction of the ductus arteriosus.
Conclusion: The ductus arteriosus of the term newborn pig, in contrast to that of the fetus, expresses cyclooxygenase-2, but circulating prostaglandins, arising mostly from cyclooxygenase-1, seem to exert the major control on ductal patency in vivo. Our data suggest that cyclooxygenase-2 inhibitors might be better alternatives for the fetus than nonselective cyclooxygenase blockers if indicated for maternal conditions such as inflammation or for tocolysis. (Am J Obstet Gynecol 1998;179:1618-26.)</abstract><cop>Philadelphia, PA</cop><pub>Mosby, Inc</pub><pmid>9855607</pmid><doi>10.1016/S0002-9378(98)70035-3</doi><tpages>9</tpages></addata></record> |
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| ispartof | American journal of obstetrics and gynecology, 1998-12, Vol.179 (6), p.1618-1626 |
| issn | 0002-9378 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Newborn - metabolism Biological and medical sciences cyclooxygenase Cyclooxygenase 1 Cyclooxygenase 2 Dinoprostone - biosynthesis Ductus arteriosus Ductus Arteriosus - drug effects Ductus Arteriosus - enzymology Ductus Arteriosus - metabolism Enzyme Inhibitors - pharmacology fetus Fetus - blood supply Fetus - enzymology Fundamental and applied biological sciences. Psychology Isoenzymes - metabolism Mother. Fetoplacental unit. Mammary gland. Milk newborn Pregnancy. Parturition. Lactation Prostaglandin-Endoperoxide Synthases - metabolism prostaglandins Swine Vertebrates: reproduction |
| title | Expression of cyclooxygenases in ductus arteriosus of fetal and newborn pigs |
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