Evaluation of Quantitative Methods for the Determination of Polyphenols in Algal Extracts
Marine brown algae such as Ascophyllum nodosum and Fucus vesiculosus accumulate polyphenols composed of phloroglucinol units. These compounds are of ecological importance and, due to their antioxidative activity, of pharmacological value as well. In this study four methods for the quantitative deter...
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| Veröffentlicht in: | Journal of natural products (Washington, D.C.) D.C.), 2007-12, Vol.70 (12), p.1865-1870 |
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| container_title | Journal of natural products (Washington, D.C.) |
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| creator | Parys, Sabine Rosenbaum, Anne Kehraus, Stefan Reher, Gerrit Glombitza, Karl-Werner König, Gabriele M |
| description | Marine brown algae such as Ascophyllum nodosum and Fucus vesiculosus accumulate polyphenols composed of phloroglucinol units. These compounds are of ecological importance and, due to their antioxidative activity, of pharmacological value as well. In this study four methods for the quantitative determination of phlorotannins are compared: spectrophotometric determinations using Folin−Ciocalteuʼs phenol reagent or 2,4-dimethoxybenzaldehyde (DMBA), quantitative 1H NMR spectroscopy (qHNMR), and gravimetrical measurements. On the basis of the relative standard deviation and the F-test, the determination using Folin−Ciocalteuʼs phenol reagent and qHNMR proved to be the most reliable and precise methods. |
| doi_str_mv | 10.1021/np070302f |
| format | Article |
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These compounds are of ecological importance and, due to their antioxidative activity, of pharmacological value as well. In this study four methods for the quantitative determination of phlorotannins are compared: spectrophotometric determinations using Folin−Ciocalteuʼs phenol reagent or 2,4-dimethoxybenzaldehyde (DMBA), quantitative 1H NMR spectroscopy (qHNMR), and gravimetrical measurements. On the basis of the relative standard deviation and the F-test, the determination using Folin−Ciocalteuʼs phenol reagent and qHNMR proved to be the most reliable and precise methods.</description><identifier>ISSN: 0163-3864</identifier><identifier>EISSN: 1520-6025</identifier><identifier>DOI: 10.1021/np070302f</identifier><identifier>PMID: 18052031</identifier><identifier>CODEN: JNPRDF</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society and American Society of Pharmacognosy</publisher><subject>algae and seaweeds ; Antioxidants - analysis ; Antioxidants - chemistry ; Antioxidants - isolation & purification ; Antioxidants - pharmacology ; Ascophyllum - chemistry ; Ascophyllum nodosum ; Biological and medical sciences ; chemical analysis ; Flavonoids - analysis ; Flavonoids - chemistry ; Flavonoids - isolation & purification ; Flavonoids - pharmacology ; France ; Fucaceae ; Fucus - chemistry ; Fucus vesiculosus ; General pharmacology ; Marine Biology ; Medical sciences ; Molecular Structure ; Nuclear Magnetic Resonance, Biomolecular ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Phenols - analysis ; Phenols - chemistry ; Phenols - isolation & purification ; Phenols - pharmacology ; phloroglucinol ; phlorotannin ; Polyphenols ; quantitative analysis ; spectral analysis ; tannins ; Tannins - analysis ; Tannins - chemistry ; Tannins - isolation & purification ; Tannins - pharmacology</subject><ispartof>Journal of natural products (Washington, D.C.), 2007-12, Vol.70 (12), p.1865-1870</ispartof><rights>Copyright © 2007 American Chemical Society and American Society of Pharmacognosy and American Society of Pharmacognosy</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a502t-88e4c35f28a90e544cdfd5b09c71a770e3fb3cb7017d755ccccbcfe3f48c80de3</citedby><cites>FETCH-LOGICAL-a502t-88e4c35f28a90e544cdfd5b09c71a770e3fb3cb7017d755ccccbcfe3f48c80de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/np070302f$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/np070302f$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20021302$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18052031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parys, Sabine</creatorcontrib><creatorcontrib>Rosenbaum, Anne</creatorcontrib><creatorcontrib>Kehraus, Stefan</creatorcontrib><creatorcontrib>Reher, Gerrit</creatorcontrib><creatorcontrib>Glombitza, Karl-Werner</creatorcontrib><creatorcontrib>König, Gabriele M</creatorcontrib><title>Evaluation of Quantitative Methods for the Determination of Polyphenols in Algal Extracts</title><title>Journal of natural products (Washington, D.C.)</title><addtitle>J. Nat. Prod</addtitle><description>Marine brown algae such as Ascophyllum nodosum and Fucus vesiculosus accumulate polyphenols composed of phloroglucinol units. These compounds are of ecological importance and, due to their antioxidative activity, of pharmacological value as well. In this study four methods for the quantitative determination of phlorotannins are compared: spectrophotometric determinations using Folin−Ciocalteuʼs phenol reagent or 2,4-dimethoxybenzaldehyde (DMBA), quantitative 1H NMR spectroscopy (qHNMR), and gravimetrical measurements. On the basis of the relative standard deviation and the F-test, the determination using Folin−Ciocalteuʼs phenol reagent and qHNMR proved to be the most reliable and precise methods.</description><subject>algae and seaweeds</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - isolation & purification</subject><subject>Antioxidants - pharmacology</subject><subject>Ascophyllum - chemistry</subject><subject>Ascophyllum nodosum</subject><subject>Biological and medical sciences</subject><subject>chemical analysis</subject><subject>Flavonoids - analysis</subject><subject>Flavonoids - chemistry</subject><subject>Flavonoids - isolation & purification</subject><subject>Flavonoids - pharmacology</subject><subject>France</subject><subject>Fucaceae</subject><subject>Fucus - chemistry</subject><subject>Fucus vesiculosus</subject><subject>General pharmacology</subject><subject>Marine Biology</subject><subject>Medical sciences</subject><subject>Molecular Structure</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenols - analysis</subject><subject>Phenols - chemistry</subject><subject>Phenols - isolation & purification</subject><subject>Phenols - pharmacology</subject><subject>phloroglucinol</subject><subject>phlorotannin</subject><subject>Polyphenols</subject><subject>quantitative analysis</subject><subject>spectral analysis</subject><subject>tannins</subject><subject>Tannins - analysis</subject><subject>Tannins - chemistry</subject><subject>Tannins - isolation & purification</subject><subject>Tannins - pharmacology</subject><issn>0163-3864</issn><issn>1520-6025</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UFvFCEYBmBiNHatHvwDykWTHkY_YBiYY1O31rjGNrs9eCIMA92ps7AC07T_XprdbC8mciHAk5fwgtBbAp8IUPLZb0EAA-qeoRnhFKoGKH-OZkAaVjHZ1EfoVUq3AAW1_CU6IhIKY2SGfs3v9DjpPASPg8NXk_Z5yGV9Z_EPm9ehT9iFiPPa4i8227gZ_EFfhvFhu7Y-jAkPHp-ON3rE8_sctcnpNXrh9Jjsm_18jK7P56uzi2rx8-u3s9NFpTnQXElpa8O4o1K3YHldm971vIPWCKKFAMtcx0wngIhecG7K6Iwru7U0EnrLjtHHXe42hj-TTVlthmTsOGpvw5RU04JkVPL_QtI2wFrJCjzZQRNDStE6tY3DRscHRUA9Fq4OhRf7bh86dRvbP8l9wwV82AOdjB5d1N4M6eAolLySVFy1c0PK9v5wruNv1QgmuFpdLtXF98XqqmmX6rz49zvvdFD6JpbM6yWFkgVStA3nTzdrk9RtmKIv3_CPJ_wFXRutQw</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Parys, Sabine</creator><creator>Rosenbaum, Anne</creator><creator>Kehraus, Stefan</creator><creator>Reher, Gerrit</creator><creator>Glombitza, Karl-Werner</creator><creator>König, Gabriele M</creator><general>American Chemical Society and American Society of Pharmacognosy</general><general>American Society of Pharmacognosy</general><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Evaluation of Quantitative Methods for the Determination of Polyphenols in Algal Extracts</title><author>Parys, Sabine ; Rosenbaum, Anne ; Kehraus, Stefan ; Reher, Gerrit ; Glombitza, Karl-Werner ; König, Gabriele M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a502t-88e4c35f28a90e544cdfd5b09c71a770e3fb3cb7017d755ccccbcfe3f48c80de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>algae and seaweeds</topic><topic>Antioxidants - analysis</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - isolation & purification</topic><topic>Antioxidants - pharmacology</topic><topic>Ascophyllum - chemistry</topic><topic>Ascophyllum nodosum</topic><topic>Biological and medical sciences</topic><topic>chemical analysis</topic><topic>Flavonoids - analysis</topic><topic>Flavonoids - chemistry</topic><topic>Flavonoids - isolation & purification</topic><topic>Flavonoids - pharmacology</topic><topic>France</topic><topic>Fucaceae</topic><topic>Fucus - chemistry</topic><topic>Fucus vesiculosus</topic><topic>General pharmacology</topic><topic>Marine Biology</topic><topic>Medical sciences</topic><topic>Molecular Structure</topic><topic>Nuclear Magnetic Resonance, Biomolecular</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenols - analysis</topic><topic>Phenols - chemistry</topic><topic>Phenols - isolation & purification</topic><topic>Phenols - pharmacology</topic><topic>phloroglucinol</topic><topic>phlorotannin</topic><topic>Polyphenols</topic><topic>quantitative analysis</topic><topic>spectral analysis</topic><topic>tannins</topic><topic>Tannins - analysis</topic><topic>Tannins - chemistry</topic><topic>Tannins - isolation & purification</topic><topic>Tannins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parys, Sabine</creatorcontrib><creatorcontrib>Rosenbaum, Anne</creatorcontrib><creatorcontrib>Kehraus, Stefan</creatorcontrib><creatorcontrib>Reher, Gerrit</creatorcontrib><creatorcontrib>Glombitza, Karl-Werner</creatorcontrib><creatorcontrib>König, Gabriele M</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of natural products (Washington, D.C.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parys, Sabine</au><au>Rosenbaum, Anne</au><au>Kehraus, Stefan</au><au>Reher, Gerrit</au><au>Glombitza, Karl-Werner</au><au>König, Gabriele M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Quantitative Methods for the Determination of Polyphenols in Algal Extracts</atitle><jtitle>Journal of natural products (Washington, D.C.)</jtitle><addtitle>J. Nat. Prod</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>70</volume><issue>12</issue><spage>1865</spage><epage>1870</epage><pages>1865-1870</pages><issn>0163-3864</issn><eissn>1520-6025</eissn><coden>JNPRDF</coden><abstract>Marine brown algae such as Ascophyllum nodosum and Fucus vesiculosus accumulate polyphenols composed of phloroglucinol units. These compounds are of ecological importance and, due to their antioxidative activity, of pharmacological value as well. In this study four methods for the quantitative determination of phlorotannins are compared: spectrophotometric determinations using Folin−Ciocalteuʼs phenol reagent or 2,4-dimethoxybenzaldehyde (DMBA), quantitative 1H NMR spectroscopy (qHNMR), and gravimetrical measurements. On the basis of the relative standard deviation and the F-test, the determination using Folin−Ciocalteuʼs phenol reagent and qHNMR proved to be the most reliable and precise methods.</abstract><cop>Washington, DC</cop><cop>Glendale, AZ</cop><pub>American Chemical Society and American Society of Pharmacognosy</pub><pmid>18052031</pmid><doi>10.1021/np070302f</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0163-3864 |
| ispartof | Journal of natural products (Washington, D.C.), 2007-12, Vol.70 (12), p.1865-1870 |
| issn | 0163-3864 1520-6025 |
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| subjects | algae and seaweeds Antioxidants - analysis Antioxidants - chemistry Antioxidants - isolation & purification Antioxidants - pharmacology Ascophyllum - chemistry Ascophyllum nodosum Biological and medical sciences chemical analysis Flavonoids - analysis Flavonoids - chemistry Flavonoids - isolation & purification Flavonoids - pharmacology France Fucaceae Fucus - chemistry Fucus vesiculosus General pharmacology Marine Biology Medical sciences Molecular Structure Nuclear Magnetic Resonance, Biomolecular Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Phenols - analysis Phenols - chemistry Phenols - isolation & purification Phenols - pharmacology phloroglucinol phlorotannin Polyphenols quantitative analysis spectral analysis tannins Tannins - analysis Tannins - chemistry Tannins - isolation & purification Tannins - pharmacology |
| title | Evaluation of Quantitative Methods for the Determination of Polyphenols in Algal Extracts |
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