Simultaneous Measurements of Cytosolic and Mitochondrial Ca2+ Transients in HT29 Cells

Loading of HT 29 cells with the Ca 2+ dye fura-2/AM resulted in an nonhomogeneous intracellular distribution of the dye. Cellular compartments with high fura-2 concentrations were identified by correlation with mitochondrial markers, cellular autofluorescence induced by UV, and dynamic measurement o...

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Veröffentlicht in:	The Journal of biological chemistry 1998-12, Vol.273 (52), p.34961-34969
Hauptverfasser:	Ricken, S, Leipziger, J, Greger, R, Nitschke, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Calcium Signaling Carbachol - pharmacology Cell Compartmentation Cytosol - metabolism Energy Metabolism Epithelial Cells - cytology Epithelial Cells - metabolism Fura-2 HT29 Cells Humans Image Processing, Computer-Assisted - methods Lasers Microscopy, Confocal Mitochondria - metabolism Muscarinic Agonists - pharmacology NAD - analysis NADP - analysis Neurotensin - pharmacology Photons Receptor, Muscarinic M3 Receptors, Muscarinic - metabolism Thapsigargin - pharmacology
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