West Nile virus in Southern Portugal, 2004
West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fra...
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| Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2005-12, Vol.5 (4), p.410-413 |
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| container_title | Vector borne and zoonotic diseases (Larchmont, N.Y.) |
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| creator | Esteves, A Almeida, A.P.G Galao, R.P Parreira, R Piedade, J Rodrigues, J.C Sousa, C.A Novo, M.T |
| description | West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR)
in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection
rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region,
amplified from
Culex pipiens
s.l. and
Culex univittatus
unfed females, demonstrated a close relationship with
WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this
short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent
surveillance for viral activity within the country.
Vector-Borne Zoonotic Dis. 5, 410-413. |
| doi_str_mv | 10.1089/vbz.2005.5.410 |
| format | Article |
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in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection
rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region,
amplified from
Culex pipiens
s.l. and
Culex univittatus
unfed females, demonstrated a close relationship with
WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this
short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent
surveillance for viral activity within the country.
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in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection
rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region,
amplified from
Culex pipiens
s.l. and
Culex univittatus
unfed females, demonstrated a close relationship with
WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this
short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent
surveillance for viral activity within the country.
Vector-Borne Zoonotic Dis. 5, 410-413.</description><subject>Animals</subject><subject>Culex pipiens</subject><subject>Culex univittatus</subject><subject>Culicidae</subject><subject>Culicidae - virology</subject><subject>disease detection</subject><subject>DNA Primers - chemistry</subject><subject>epidemiological studies</subject><subject>Female</subject><subject>infection rate</subject><subject>insect vectors</subject><subject>Insect Vectors - virology</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Portugal</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Viral - chemistry</subject><subject>sequence homology</subject><subject>Short Reports</subject><subject>Viral Nonstructural Proteins - genetics</subject><subject>West Nile virus</subject><subject>West Nile virus - classification</subject><subject>West Nile virus - genetics</subject><subject>West Nile virus - isolation & purification</subject><issn>1530-3667</issn><issn>1557-7759</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EoqWwMkImBtSEsx3HzogqvqQKkErFaDmOXYLSpNhJJfj1JEoFAwPTnU7PvXd6EDrFEGEQ6dU2-4oIAItYFGPYQ2PMGA85Z-l-31MIaZLwETry_h2AYIHZIRrhJMY8pnyMLl-Nb4LHojTBtnCtD4oqWNRt82ZcFTzXrmlXqpwG3Yn4GB1YVXpzsqsTtLy9eZndh_Onu4fZ9TzUVLAmxCqlTBuTYUJTBpkRVFtK0jgDMCRPbJznYA1LdC4gZ1hrkoFNGGgruCI5naCLIXfj6o-2e0-uC69NWarK1K2XSQqccCr-BTEnlMYcOjAaQO1q752xcuOKtXKfEoPsNcpOo-w1SiY7jd3C2S65zdYm_8V33jpgOgD9WFVVWZjMuOYH_JN3PuBW1VKtXOHlckEAU8DABIkJ_QYNY4Sj</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Esteves, A</creator><creator>Almeida, A.P.G</creator><creator>Galao, R.P</creator><creator>Parreira, R</creator><creator>Piedade, J</creator><creator>Rodrigues, J.C</creator><creator>Sousa, C.A</creator><creator>Novo, M.T</creator><general>Mary Ann Liebert, Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>West Nile virus in Southern Portugal, 2004</title><author>Esteves, A ; Almeida, A.P.G ; Galao, R.P ; Parreira, R ; Piedade, J ; Rodrigues, J.C ; Sousa, C.A ; Novo, M.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-1a935ceeb123950be83cf3294b00e2d6f4dd0fe56cd80d51cc2b0f650cf87a2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Culex pipiens</topic><topic>Culex univittatus</topic><topic>Culicidae</topic><topic>Culicidae - virology</topic><topic>disease detection</topic><topic>DNA Primers - chemistry</topic><topic>epidemiological studies</topic><topic>Female</topic><topic>infection rate</topic><topic>insect vectors</topic><topic>Insect Vectors - virology</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Portugal</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Viral - chemistry</topic><topic>sequence homology</topic><topic>Short Reports</topic><topic>Viral Nonstructural Proteins - genetics</topic><topic>West Nile virus</topic><topic>West Nile virus - classification</topic><topic>West Nile virus - genetics</topic><topic>West Nile virus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Esteves, A</creatorcontrib><creatorcontrib>Almeida, A.P.G</creatorcontrib><creatorcontrib>Galao, R.P</creatorcontrib><creatorcontrib>Parreira, R</creatorcontrib><creatorcontrib>Piedade, J</creatorcontrib><creatorcontrib>Rodrigues, J.C</creatorcontrib><creatorcontrib>Sousa, C.A</creatorcontrib><creatorcontrib>Novo, M.T</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Esteves, A</au><au>Almeida, A.P.G</au><au>Galao, R.P</au><au>Parreira, R</au><au>Piedade, J</au><au>Rodrigues, J.C</au><au>Sousa, C.A</au><au>Novo, M.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>West Nile virus in Southern Portugal, 2004</atitle><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle><addtitle>Vector Borne Zoonotic Dis</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>5</volume><issue>4</issue><spage>410</spage><epage>413</epage><pages>410-413</pages><issn>1530-3667</issn><eissn>1557-7759</eissn><abstract>West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR)
in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection
rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region,
amplified from
Culex pipiens
s.l. and
Culex univittatus
unfed females, demonstrated a close relationship with
WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this
short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent
surveillance for viral activity within the country.
Vector-Borne Zoonotic Dis. 5, 410-413.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>16417437</pmid><doi>10.1089/vbz.2005.5.410</doi><tpages>4</tpages></addata></record> |
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| issn | 1530-3667 1557-7759 |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Animals Culex pipiens Culex univittatus Culicidae Culicidae - virology disease detection DNA Primers - chemistry epidemiological studies Female infection rate insect vectors Insect Vectors - virology Molecular Sequence Data nucleotide sequences Phylogeny Portugal Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - chemistry sequence homology Short Reports Viral Nonstructural Proteins - genetics West Nile virus West Nile virus - classification West Nile virus - genetics West Nile virus - isolation & purification |
| title | West Nile virus in Southern Portugal, 2004 |
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