DIFFERENCES IN HSP70 EXPRESSION IN THE SPOROZOITES OF THE ORIGINAL STRAIN AND PRECOCIOUS LINES OF EIMERIA TENELLA

The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was...

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Veröffentlicht in:The Journal of parasitology 2005-10, Vol.91 (5), p.1127-1131
Hauptverfasser: del Cacho, E, Gallego, M, López-Bernad, F, Quílez, J, Sánchez-Acedo, C
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container_issue 5
container_start_page 1127
container_title The Journal of parasitology
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creator del Cacho, E
Gallego, M
López-Bernad, F
Quílez, J
Sánchez-Acedo, C
description The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.
doi_str_mv 10.1645/GE-3464.1
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Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.1645/GE-3464.1</identifier><identifier>PMID: 16419759</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Animals ; Binding sites ; Biological and medical sciences ; Chickens - parasitology ; Coccidiosis ; Coccidiosis - parasitology ; Coccidiosis - prevention &amp; control ; Coccidiosis - veterinary ; Densitometers ; Densitometry - veterinary ; Eimeria tenella ; Eimeria tenella - genetics ; Eimeria tenella - immunology ; Eimeria tenella - metabolism ; Flow cytometry ; Flow Cytometry - veterinary ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; General aspects ; General aspects and techniques. Study of several systematic groups. 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Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>16419759</pmid><doi>10.1645/GE-3464.1</doi><tpages>5</tpages></addata></record>
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subjects Animals
Binding sites
Biological and medical sciences
Chickens - parasitology
Coccidiosis
Coccidiosis - parasitology
Coccidiosis - prevention & control
Coccidiosis - veterinary
Densitometers
Densitometry - veterinary
Eimeria tenella
Eimeria tenella - genetics
Eimeria tenella - immunology
Eimeria tenella - metabolism
Flow cytometry
Flow Cytometry - veterinary
Fluorescence
Fundamental and applied biological sciences. Psychology
Gene Expression
General aspects
General aspects and techniques. Study of several systematic groups. Models
heat shock protein 70
Heat shock proteins
HSP70 Heat-Shock Proteins - biosynthesis
HSP70 Heat-Shock Proteins - immunology
Hsp70 protein
Immunoblotting - veterinary
Immunohistochemistry - veterinary
Invertebrates
line differences
Monoclonal antibodies
Oocysts
Optical density
Parasites
Parasitology
Pathogenicity
Pathogens
PATHOLOGY
Poultry Diseases - parasitology
Poultry Diseases - prevention & control
protein synthesis
Proteins
Protozoan Vaccines
spatial distribution
Sporozoites
Sporozoites - genetics
Sporozoites - immunology
Sporozoites - metabolism
strain differences
Transcriptional regulatory elements
Vaccines, Attenuated
Virulence
title DIFFERENCES IN HSP70 EXPRESSION IN THE SPOROZOITES OF THE ORIGINAL STRAIN AND PRECOCIOUS LINES OF EIMERIA TENELLA
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