DIFFERENCES IN HSP70 EXPRESSION IN THE SPOROZOITES OF THE ORIGINAL STRAIN AND PRECOCIOUS LINES OF EIMERIA TENELLA
The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was...
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description | The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines. |
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Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.1645/GE-3464.1</identifier><identifier>PMID: 16419759</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Animals ; Binding sites ; Biological and medical sciences ; Chickens - parasitology ; Coccidiosis ; Coccidiosis - parasitology ; Coccidiosis - prevention & control ; Coccidiosis - veterinary ; Densitometers ; Densitometry - veterinary ; Eimeria tenella ; Eimeria tenella - genetics ; Eimeria tenella - immunology ; Eimeria tenella - metabolism ; Flow cytometry ; Flow Cytometry - veterinary ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; heat shock protein 70 ; Heat shock proteins ; HSP70 Heat-Shock Proteins - biosynthesis ; HSP70 Heat-Shock Proteins - immunology ; Hsp70 protein ; Immunoblotting - veterinary ; Immunohistochemistry - veterinary ; Invertebrates ; line differences ; Monoclonal antibodies ; Oocysts ; Optical density ; Parasites ; Parasitology ; Pathogenicity ; Pathogens ; PATHOLOGY ; Poultry Diseases - parasitology ; Poultry Diseases - prevention & control ; protein synthesis ; Proteins ; Protozoan Vaccines ; spatial distribution ; Sporozoites ; Sporozoites - genetics ; Sporozoites - immunology ; Sporozoites - metabolism ; strain differences ; Transcriptional regulatory elements ; Vaccines, Attenuated ; Virulence</subject><ispartof>The Journal of parasitology, 2005-10, Vol.91 (5), p.1127-1131</ispartof><rights>American Society of Parasitologists</rights><rights>Copyright 2005 American Society of Parasitologists</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Allen Press Inc. Oct 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b419t-6fc6e6e96ce1c95c0cb204acd69befd84b6f79d5f5365fb241c15fdaab2738b03</citedby><cites>FETCH-LOGICAL-b419t-6fc6e6e96ce1c95c0cb204acd69befd84b6f79d5f5365fb241c15fdaab2738b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1645/GE-3464.1$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20059830$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,26978,27924,27925,52363,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17290208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16419759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>del Cacho, E</creatorcontrib><creatorcontrib>Gallego, M</creatorcontrib><creatorcontrib>López-Bernad, F</creatorcontrib><creatorcontrib>Quílez, J</creatorcontrib><creatorcontrib>Sánchez-Acedo, C</creatorcontrib><title>DIFFERENCES IN HSP70 EXPRESSION IN THE SPOROZOITES OF THE ORIGINAL STRAIN AND PRECOCIOUS LINES OF EIMERIA TENELLA</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.</description><subject>Animals</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Chickens - parasitology</subject><subject>Coccidiosis</subject><subject>Coccidiosis - parasitology</subject><subject>Coccidiosis - prevention & control</subject><subject>Coccidiosis - veterinary</subject><subject>Densitometers</subject><subject>Densitometry - veterinary</subject><subject>Eimeria tenella</subject><subject>Eimeria tenella - genetics</subject><subject>Eimeria tenella - immunology</subject><subject>Eimeria tenella - metabolism</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - veterinary</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. 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Psychology</topic><topic>Gene Expression</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>heat shock protein 70</topic><topic>Heat shock proteins</topic><topic>HSP70 Heat-Shock Proteins - biosynthesis</topic><topic>HSP70 Heat-Shock Proteins - immunology</topic><topic>Hsp70 protein</topic><topic>Immunoblotting - veterinary</topic><topic>Immunohistochemistry - veterinary</topic><topic>Invertebrates</topic><topic>line differences</topic><topic>Monoclonal antibodies</topic><topic>Oocysts</topic><topic>Optical density</topic><topic>Parasites</topic><topic>Parasitology</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>PATHOLOGY</topic><topic>Poultry Diseases - parasitology</topic><topic>Poultry Diseases - prevention & control</topic><topic>protein synthesis</topic><topic>Proteins</topic><topic>Protozoan Vaccines</topic><topic>spatial distribution</topic><topic>Sporozoites</topic><topic>Sporozoites - genetics</topic><topic>Sporozoites - immunology</topic><topic>Sporozoites - metabolism</topic><topic>strain differences</topic><topic>Transcriptional regulatory elements</topic><topic>Vaccines, Attenuated</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>del Cacho, E</creatorcontrib><creatorcontrib>Gallego, M</creatorcontrib><creatorcontrib>López-Bernad, F</creatorcontrib><creatorcontrib>Quílez, J</creatorcontrib><creatorcontrib>Sánchez-Acedo, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>del Cacho, E</au><au>Gallego, M</au><au>López-Bernad, F</au><au>Quílez, J</au><au>Sánchez-Acedo, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DIFFERENCES IN HSP70 EXPRESSION IN THE SPOROZOITES OF THE ORIGINAL STRAIN AND PRECOCIOUS LINES OF EIMERIA TENELLA</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>91</volume><issue>5</issue><spage>1127</spage><epage>1131</epage><pages>1127-1131</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>16419759</pmid><doi>10.1645/GE-3464.1</doi><tpages>5</tpages></addata></record> |
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subjects | Animals Binding sites Biological and medical sciences Chickens - parasitology Coccidiosis Coccidiosis - parasitology Coccidiosis - prevention & control Coccidiosis - veterinary Densitometers Densitometry - veterinary Eimeria tenella Eimeria tenella - genetics Eimeria tenella - immunology Eimeria tenella - metabolism Flow cytometry Flow Cytometry - veterinary Fluorescence Fundamental and applied biological sciences. Psychology Gene Expression General aspects General aspects and techniques. Study of several systematic groups. Models heat shock protein 70 Heat shock proteins HSP70 Heat-Shock Proteins - biosynthesis HSP70 Heat-Shock Proteins - immunology Hsp70 protein Immunoblotting - veterinary Immunohistochemistry - veterinary Invertebrates line differences Monoclonal antibodies Oocysts Optical density Parasites Parasitology Pathogenicity Pathogens PATHOLOGY Poultry Diseases - parasitology Poultry Diseases - prevention & control protein synthesis Proteins Protozoan Vaccines spatial distribution Sporozoites Sporozoites - genetics Sporozoites - immunology Sporozoites - metabolism strain differences Transcriptional regulatory elements Vaccines, Attenuated Virulence |
title | DIFFERENCES IN HSP70 EXPRESSION IN THE SPOROZOITES OF THE ORIGINAL STRAIN AND PRECOCIOUS LINES OF EIMERIA TENELLA |
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