All Optical Interface for Parallel, Remote, and Spatiotemporal Control of Neuronal Activity
A key technical barrier to furthering our understanding of complex neural networks has been the lack of tools for the simultaneous spatiotemporal control and detection of activity in a large number of neurons. Here, we report an all-optical system for achieving this kind of parallel and selective co...
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| Veröffentlicht in: | Nano letters 2007-12, Vol.7 (12), p.3859-3863 |
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| container_issue | 12 |
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| container_title | Nano letters |
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| creator | Wang, Sheng Szobota, Stephanie Wang, Yuan Volgraf, Matthew Liu, Zhaowei Sun, Cheng Trauner, Dirk Isacoff, Ehud Y Zhang, Xiang |
| description | A key technical barrier to furthering our understanding of complex neural networks has been the lack of tools for the simultaneous spatiotemporal control and detection of activity in a large number of neurons. Here, we report an all-optical system for achieving this kind of parallel and selective control and detection. We do this by delivering spatiotemporally complex optical stimuli through a digital micromirror spatiotemporal light modulator to cells expressing the light-activated ionotropic glutamate receptor (LiGluR), which have been labeled with a calcium dye to provide a fluorescent report of activity. Reliable and accurate spatiotemporal stimulation was obtained on HEK293 cells and cultured rat hippocampal neurons. This technique should be adaptable to in vivo applications and could serve as an optical interface for communicating with complex neural circuits. |
| doi_str_mv | 10.1021/nl072783t |
| format | Article |
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Here, we report an all-optical system for achieving this kind of parallel and selective control and detection. We do this by delivering spatiotemporally complex optical stimuli through a digital micromirror spatiotemporal light modulator to cells expressing the light-activated ionotropic glutamate receptor (LiGluR), which have been labeled with a calcium dye to provide a fluorescent report of activity. Reliable and accurate spatiotemporal stimulation was obtained on HEK293 cells and cultured rat hippocampal neurons. This technique should be adaptable to in vivo applications and could serve as an optical interface for communicating with complex neural circuits.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/nl072783t</identifier><identifier>PMID: 18034506</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Biological and medical sciences ; Calcium - physiology ; Cell Line ; Cells, Cultured ; Fundamental and applied biological sciences. Psychology ; General aspects ; Hippocampus - physiology ; Humans ; Instrumentation. Materials. Reagents. 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Psychology</subject><subject>General aspects</subject><subject>Hippocampus - physiology</subject><subject>Humans</subject><subject>Instrumentation. Materials. Reagents. Research laboratory organization</subject><subject>Kidney - cytology</subject><subject>Kidney - physiology</subject><subject>Light</subject><subject>Neurons - physiology</subject><subject>Rats</subject><subject>Receptors, AMPA - physiology</subject><subject>Receptors, Metabotropic Glutamate - physiology</subject><subject>Receptors, Metabotropic Glutamate - radiation effects</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUtLAzEQgIMotj4O_gHJRUFoNclm0-RYio9CUfFx8rBMdxPYkt2sSVbw3xvpYi-e5vUxA98gdEbJNSWM3rSWzNhMZnEPjWmekalQiu3_5ZKP0FEIG0KIynJyiEZUkoznRIzRx9xa_NTFugSLl23U3kCpsXEeP4MHa7Wd4BfduKgnGNoKv3YQ61Q1nUtjvHBt9M5iZ_Cj7r1rU29exvqrjt8n6MCADfp0iMfo_e72bfEwXT3dLxfz1RQ4UXFKSaUEU6XI11yKykidCT4DQSnL80wwwxQXwLk0oEzFtBCzXKyZNJJxveaQHaPL7d7Ou89eh1g0dSi1tdBq14dCKJJLQVgCr7Zg6V0IXpui83UD_rugpPg1WfyZTOz5sLRfN7rakYO6BFwMAIQkz3hoyzrsuPSC5JvvOChDsXG9T4rCPwd_AL_PhhU</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Wang, Sheng</creator><creator>Szobota, Stephanie</creator><creator>Wang, Yuan</creator><creator>Volgraf, Matthew</creator><creator>Liu, Zhaowei</creator><creator>Sun, Cheng</creator><creator>Trauner, Dirk</creator><creator>Isacoff, Ehud Y</creator><creator>Zhang, Xiang</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>All Optical Interface for Parallel, Remote, and Spatiotemporal Control of Neuronal Activity</title><author>Wang, Sheng ; Szobota, Stephanie ; Wang, Yuan ; Volgraf, Matthew ; Liu, Zhaowei ; Sun, Cheng ; Trauner, Dirk ; Isacoff, Ehud Y ; Zhang, Xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a409t-10d9629c65b486df8e3647a611255362f2946a448fa9fd2e66756b28f824eb4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - physiology</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Hippocampus - physiology</topic><topic>Humans</topic><topic>Instrumentation. Materials. Reagents. Research laboratory organization</topic><topic>Kidney - cytology</topic><topic>Kidney - physiology</topic><topic>Light</topic><topic>Neurons - physiology</topic><topic>Rats</topic><topic>Receptors, AMPA - physiology</topic><topic>Receptors, Metabotropic Glutamate - physiology</topic><topic>Receptors, Metabotropic Glutamate - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Sheng</creatorcontrib><creatorcontrib>Szobota, Stephanie</creatorcontrib><creatorcontrib>Wang, Yuan</creatorcontrib><creatorcontrib>Volgraf, Matthew</creatorcontrib><creatorcontrib>Liu, Zhaowei</creatorcontrib><creatorcontrib>Sun, Cheng</creatorcontrib><creatorcontrib>Trauner, Dirk</creatorcontrib><creatorcontrib>Isacoff, Ehud Y</creatorcontrib><creatorcontrib>Zhang, Xiang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Sheng</au><au>Szobota, Stephanie</au><au>Wang, Yuan</au><au>Volgraf, Matthew</au><au>Liu, Zhaowei</au><au>Sun, Cheng</au><au>Trauner, Dirk</au><au>Isacoff, Ehud Y</au><au>Zhang, Xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>All Optical Interface for Parallel, Remote, and Spatiotemporal Control of Neuronal Activity</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>7</volume><issue>12</issue><spage>3859</spage><epage>3863</epage><pages>3859-3863</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>A key technical barrier to furthering our understanding of complex neural networks has been the lack of tools for the simultaneous spatiotemporal control and detection of activity in a large number of neurons. 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| ispartof | Nano letters, 2007-12, Vol.7 (12), p.3859-3863 |
| issn | 1530-6984 1530-6992 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Animals Biological and medical sciences Calcium - physiology Cell Line Cells, Cultured Fundamental and applied biological sciences. Psychology General aspects Hippocampus - physiology Humans Instrumentation. Materials. Reagents. Research laboratory organization Kidney - cytology Kidney - physiology Light Neurons - physiology Rats Receptors, AMPA - physiology Receptors, Metabotropic Glutamate - physiology Receptors, Metabotropic Glutamate - radiation effects |
| title | All Optical Interface for Parallel, Remote, and Spatiotemporal Control of Neuronal Activity |
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