Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation
The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse...
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| Veröffentlicht in: | Biometals 2005-12, Vol.18 (6), p.577-585 |
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| creator | Koppisch, Andrew T Browder, Cindy C Moe, Amanda L Shelley, Jacob T Kinkel, Blaine A Hersman, Larry E Iyer, Srinivas Ruggiero, Christy E |
| description | The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen. |
| doi_str_mv | 10.1007/s10534-005-1782-6 |
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Sterne under conditions of iron starvation</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Koppisch, Andrew T ; Browder, Cindy C ; Moe, Amanda L ; Shelley, Jacob T ; Kinkel, Blaine A ; Hersman, Larry E ; Iyer, Srinivas ; Ruggiero, Christy E</creator><creatorcontrib>Koppisch, Andrew T ; Browder, Cindy C ; Moe, Amanda L ; Shelley, Jacob T ; Kinkel, Blaine A ; Hersman, Larry E ; Iyer, Srinivas ; Ruggiero, Christy E</creatorcontrib><description>The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.</description><identifier>ISSN: 0966-0844</identifier><identifier>EISSN: 1572-8773</identifier><identifier>DOI: 10.1007/s10534-005-1782-6</identifier><identifier>PMID: 16388397</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Bacillus anthracis ; Bacillus anthracis - chemistry ; Bacillus anthracis - metabolism ; Bacteria ; Benzamides - chemistry ; Benzamides - isolation & purification ; Benzamides - metabolism ; Cells, Cultured ; Esters - chemistry ; Esters - isolation & purification ; Esters - metabolism ; Hydrolysis ; Iron - metabolism ; Marinobacter hydrocarbonoclasticus ; Microbiology ; Molecular Structure ; Oligopeptides - chemistry ; Oligopeptides - isolation & purification ; Oligopeptides - metabolism ; Pathogens ; Proteins ; Sensitivity and Specificity ; Siderophores - biosynthesis ; Siderophores - chemistry ; Siderophores - isolation & purification ; Species Specificity ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Structural analysis ; Ultraviolet spectroscopy</subject><ispartof>Biometals, 2005-12, Vol.18 (6), p.577-585</ispartof><rights>Springer 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-f05be25eb0e588e743759fc682cca805343b94299de88912cf5b44f296bc9b333</citedby><cites>FETCH-LOGICAL-c357t-f05be25eb0e588e743759fc682cca805343b94299de88912cf5b44f296bc9b333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16388397$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koppisch, Andrew T</creatorcontrib><creatorcontrib>Browder, Cindy C</creatorcontrib><creatorcontrib>Moe, Amanda L</creatorcontrib><creatorcontrib>Shelley, Jacob T</creatorcontrib><creatorcontrib>Kinkel, Blaine A</creatorcontrib><creatorcontrib>Hersman, Larry E</creatorcontrib><creatorcontrib>Iyer, Srinivas</creatorcontrib><creatorcontrib>Ruggiero, Christy E</creatorcontrib><title>Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation</title><title>Biometals</title><addtitle>Biometals</addtitle><description>The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. 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Sterne under conditions of iron starvation</atitle><jtitle>Biometals</jtitle><addtitle>Biometals</addtitle><date>2005-12</date><risdate>2005</risdate><volume>18</volume><issue>6</issue><spage>577</spage><epage>585</epage><pages>577-585</pages><issn>0966-0844</issn><eissn>1572-8773</eissn><abstract>The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>16388397</pmid><doi>10.1007/s10534-005-1782-6</doi><tpages>9</tpages></addata></record> |
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| subjects | Bacillus anthracis Bacillus anthracis - chemistry Bacillus anthracis - metabolism Bacteria Benzamides - chemistry Benzamides - isolation & purification Benzamides - metabolism Cells, Cultured Esters - chemistry Esters - isolation & purification Esters - metabolism Hydrolysis Iron - metabolism Marinobacter hydrocarbonoclasticus Microbiology Molecular Structure Oligopeptides - chemistry Oligopeptides - isolation & purification Oligopeptides - metabolism Pathogens Proteins Sensitivity and Specificity Siderophores - biosynthesis Siderophores - chemistry Siderophores - isolation & purification Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Structural analysis Ultraviolet spectroscopy |
| title | Petrobactin is the primary siderophore synthesized by Bacillus anthracis str. Sterne under conditions of iron starvation |
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