Simple and sensitive quantification of bioactive peptides in biological matrices using liquid chromatography/selected reaction monitoring mass spectrometry coupled with trichloroacetic acid clean-up

A simple and sensitive liquid chromatography/electrospray ionization tandem mass spectrometric (LC/ESI‐MS/MS) method has been developed for the quantification of bioactive peptides in biological fluids. The method employs protein precipitation with 4% trichloroacetic acid (TCA) and selected reaction...

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Veröffentlicht in:	Rapid communications in mass spectrometry 2007-12, Vol.21 (24), p.4033-4038
Hauptverfasser:	Murao, Naoaki, Ishigai, Masaki, Yasuno, Hideyuki, Shimonaka, Yasushi, Aso, Yoshinori
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antimicrobial Cationic Peptides - blood Antimicrobial Cationic Peptides - chemistry Chromatography, High Pressure Liquid Hepcidins Humans Parathyroid Hormone - blood Parathyroid Hormone - chemistry Peptide Fragments - blood Peptide Fragments - chemistry Rats Reproducibility of Results Spectrometry, Mass, Electrospray Ionization - methods Tandem Mass Spectrometry - methods Trichloroacetic Acid - chemistry
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