Maternal Smoking: Effect on Circulating Cell-Free Fetal and Total DNA Levels in Maternal Plasma From the Second Trimester
To estimate whether potential clinical applications of cell-free fetal and total DNA in the field of noninvasive prenatal diagnosis need to be adjusted for maternal smoking status. In this study, using 344 maternal blood samples from the second trimester of pregnancy, circulating cell-free DNA in ma...
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| Veröffentlicht in: | Obstetrics and gynecology (New York. 1953) 2007-12, Vol.110 (6), p.1358-1363 |
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| container_title | Obstetrics and gynecology (New York. 1953) |
| container_volume | 110 |
| creator | Lapaire, Olav Volgmann, Thorsten Huang, Dorothy Hahn, Sinuhe Holzgreve, Wolfgang Xiao Zhong, Yan |
| description | To estimate whether potential clinical applications of cell-free fetal and total DNA in the field of noninvasive prenatal diagnosis need to be adjusted for maternal smoking status.
In this study, using 344 maternal blood samples from the second trimester of pregnancy, circulating cell-free DNA in maternal plasma samples, specific for the SRY and DYS14 loci (representing fetal DNA) and GAPDH sequence (representing total genomic DNA) were quantified by real-time polymerase chain reaction.
Fetal sex determination was 100% accurate using a combination of probes for SRY and DYS14. The levels of DYS14 and SRY detected were significantly correlated (r=0.884, P |
| doi_str_mv | 10.1097/01.AOG.0000295654.55971.9f |
| format | Article |
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In this study, using 344 maternal blood samples from the second trimester of pregnancy, circulating cell-free DNA in maternal plasma samples, specific for the SRY and DYS14 loci (representing fetal DNA) and GAPDH sequence (representing total genomic DNA) were quantified by real-time polymerase chain reaction.
Fetal sex determination was 100% accurate using a combination of probes for SRY and DYS14. The levels of DYS14 and SRY detected were significantly correlated (r=0.884, P<.001). No significant difference was seen between the quantitative levels of cell-free male fetal DNA between the smoking groups and control group. Similarly, no significant difference was seen in the amount of total cell-free DNA in the study population.
In contrast to first- and second-trimester screening assays for Down syndrome, where smoking status significantly affect levels of maternal serum analytes, smoking status does not affect quantitative levels of cell-free fetal DNA or total cell-free DNA in maternal plasma.
II.</description><identifier>ISSN: 0029-7844</identifier><identifier>EISSN: 1873-233X</identifier><identifier>DOI: 10.1097/01.AOG.0000295654.55971.9f</identifier><identifier>PMID: 18055732</identifier><identifier>CODEN: OBGNAS</identifier><language>eng</language><publisher>New York, NY: by The American College of Obstetricians and Gynecologists. Published by Wolters Kluwer Health, Inc. All rights reserved</publisher><subject>Adult ; Biological and medical sciences ; Biomarkers ; Case-Control Studies ; DNA - analysis ; DNA - blood ; DNA Probes ; Female ; Fetus ; Genes, sry ; Gynecology. Andrology. Obstetrics ; Humans ; Maternal-Fetal Exchange - genetics ; Medical sciences ; Pregnancy ; Pregnancy Trimester, Second ; Prenatal Diagnosis - methods ; Reproducibility of Results ; Sex Determination Analysis - methods ; Smoking - blood ; Tobacco, tobacco smoking ; Toxicology</subject><ispartof>Obstetrics and gynecology (New York. 1953), 2007-12, Vol.110 (6), p.1358-1363</ispartof><rights>by The American College of Obstetricians and Gynecologists. Published by Wolters Kluwer Health, Inc. All rights reserved.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3353-c7cf2538578101d72ad40a45b8006d64c44c3ea1683b240657134fd603cd3dba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20281062$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18055732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lapaire, Olav</creatorcontrib><creatorcontrib>Volgmann, Thorsten</creatorcontrib><creatorcontrib>Huang, Dorothy</creatorcontrib><creatorcontrib>Hahn, Sinuhe</creatorcontrib><creatorcontrib>Holzgreve, Wolfgang</creatorcontrib><creatorcontrib>Xiao Zhong, Yan</creatorcontrib><title>Maternal Smoking: Effect on Circulating Cell-Free Fetal and Total DNA Levels in Maternal Plasma From the Second Trimester</title><title>Obstetrics and gynecology (New York. 1953)</title><addtitle>Obstet Gynecol</addtitle><description>To estimate whether potential clinical applications of cell-free fetal and total DNA in the field of noninvasive prenatal diagnosis need to be adjusted for maternal smoking status.
In this study, using 344 maternal blood samples from the second trimester of pregnancy, circulating cell-free DNA in maternal plasma samples, specific for the SRY and DYS14 loci (representing fetal DNA) and GAPDH sequence (representing total genomic DNA) were quantified by real-time polymerase chain reaction.
Fetal sex determination was 100% accurate using a combination of probes for SRY and DYS14. The levels of DYS14 and SRY detected were significantly correlated (r=0.884, P<.001). No significant difference was seen between the quantitative levels of cell-free male fetal DNA between the smoking groups and control group. Similarly, no significant difference was seen in the amount of total cell-free DNA in the study population.
In contrast to first- and second-trimester screening assays for Down syndrome, where smoking status significantly affect levels of maternal serum analytes, smoking status does not affect quantitative levels of cell-free fetal DNA or total cell-free DNA in maternal plasma.
II.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Case-Control Studies</subject><subject>DNA - analysis</subject><subject>DNA - blood</subject><subject>DNA Probes</subject><subject>Female</subject><subject>Fetus</subject><subject>Genes, sry</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Maternal-Fetal Exchange - genetics</subject><subject>Medical sciences</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, Second</subject><subject>Prenatal Diagnosis - methods</subject><subject>Reproducibility of Results</subject><subject>Sex Determination Analysis - methods</subject><subject>Smoking - blood</subject><subject>Tobacco, tobacco smoking</subject><subject>Toxicology</subject><issn>0029-7844</issn><issn>1873-233X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF9v0zAUxS0EYmXwFZCFBG8J177-k-ytKutAKgxpQ-LNch2HhjnJZqdM-_Y4a7X5wbbs37nn3kPIBwYlg1p_BlYuLy9KyIvXUklRSllrVtbtC7JglcaCI_5-SRbzf6ErIU7Im5T-Zp6pGl-TE1aBlBr5gjx8t5OPgw30qh9vuuHPGT1vW-8mOg501UW3D3bKz3TlQyjW0Xu69lPG7dDQ63G-ffmxpBv_z4dEu4E-1fsZbOotXcexp9PO0yvvxlkTu96nzLwlr1obkn93PE_Jr_X59eprsbm8-LZabgqHKLFw2rVcYiV1xYA1mttGgBVyWwGoRgknhENvmapwywUoqRmKtlGArsFma_GUfDrUvY3j3T5bm75LLk9jBz_uk1E1CGSAGTw7gC6OKUXfmtvcq40PhoGZgzfATA7ePAdvHoM3dZvF748u-23vm2fpMekMfDwCNjkb2mgH16UnjgPP86mZEwfufgw5pXQT9vc-mp23Ydo9WisuoeAAmuUNirkZxP_NipqQ</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Lapaire, Olav</creator><creator>Volgmann, Thorsten</creator><creator>Huang, Dorothy</creator><creator>Hahn, Sinuhe</creator><creator>Holzgreve, Wolfgang</creator><creator>Xiao Zhong, Yan</creator><general>by The American College of Obstetricians and Gynecologists. Published by Wolters Kluwer Health, Inc. All rights reserved</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Maternal Smoking: Effect on Circulating Cell-Free Fetal and Total DNA Levels in Maternal Plasma From the Second Trimester</title><author>Lapaire, Olav ; Volgmann, Thorsten ; Huang, Dorothy ; Hahn, Sinuhe ; Holzgreve, Wolfgang ; Xiao Zhong, Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3353-c7cf2538578101d72ad40a45b8006d64c44c3ea1683b240657134fd603cd3dba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Case-Control Studies</topic><topic>DNA - analysis</topic><topic>DNA - blood</topic><topic>DNA Probes</topic><topic>Female</topic><topic>Fetus</topic><topic>Genes, sry</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Maternal-Fetal Exchange - genetics</topic><topic>Medical sciences</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, Second</topic><topic>Prenatal Diagnosis - methods</topic><topic>Reproducibility of Results</topic><topic>Sex Determination Analysis - methods</topic><topic>Smoking - blood</topic><topic>Tobacco, tobacco smoking</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lapaire, Olav</creatorcontrib><creatorcontrib>Volgmann, Thorsten</creatorcontrib><creatorcontrib>Huang, Dorothy</creatorcontrib><creatorcontrib>Hahn, Sinuhe</creatorcontrib><creatorcontrib>Holzgreve, Wolfgang</creatorcontrib><creatorcontrib>Xiao Zhong, Yan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lapaire, Olav</au><au>Volgmann, Thorsten</au><au>Huang, Dorothy</au><au>Hahn, Sinuhe</au><au>Holzgreve, Wolfgang</au><au>Xiao Zhong, Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maternal Smoking: Effect on Circulating Cell-Free Fetal and Total DNA Levels in Maternal Plasma From the Second Trimester</atitle><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle><addtitle>Obstet Gynecol</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>110</volume><issue>6</issue><spage>1358</spage><epage>1363</epage><pages>1358-1363</pages><issn>0029-7844</issn><eissn>1873-233X</eissn><coden>OBGNAS</coden><abstract>To estimate whether potential clinical applications of cell-free fetal and total DNA in the field of noninvasive prenatal diagnosis need to be adjusted for maternal smoking status.
In this study, using 344 maternal blood samples from the second trimester of pregnancy, circulating cell-free DNA in maternal plasma samples, specific for the SRY and DYS14 loci (representing fetal DNA) and GAPDH sequence (representing total genomic DNA) were quantified by real-time polymerase chain reaction.
Fetal sex determination was 100% accurate using a combination of probes for SRY and DYS14. The levels of DYS14 and SRY detected were significantly correlated (r=0.884, P<.001). No significant difference was seen between the quantitative levels of cell-free male fetal DNA between the smoking groups and control group. Similarly, no significant difference was seen in the amount of total cell-free DNA in the study population.
In contrast to first- and second-trimester screening assays for Down syndrome, where smoking status significantly affect levels of maternal serum analytes, smoking status does not affect quantitative levels of cell-free fetal DNA or total cell-free DNA in maternal plasma.
II.</abstract><cop>New York, NY</cop><pub>by The American College of Obstetricians and Gynecologists. Published by Wolters Kluwer Health, Inc. All rights reserved</pub><pmid>18055732</pmid><doi>10.1097/01.AOG.0000295654.55971.9f</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Journals@Ovid Ovid Autoload |
| subjects | Adult Biological and medical sciences Biomarkers Case-Control Studies DNA - analysis DNA - blood DNA Probes Female Fetus Genes, sry Gynecology. Andrology. Obstetrics Humans Maternal-Fetal Exchange - genetics Medical sciences Pregnancy Pregnancy Trimester, Second Prenatal Diagnosis - methods Reproducibility of Results Sex Determination Analysis - methods Smoking - blood Tobacco, tobacco smoking Toxicology |
| title | Maternal Smoking: Effect on Circulating Cell-Free Fetal and Total DNA Levels in Maternal Plasma From the Second Trimester |
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