Modeling and Parameter Identification of the Simultaneous Saccharification-Fermentation Process for Ethanol Production

Despite many environmental advantages of using alcohol as a fuel, there are still serious questions about its economical feasibility when compared with oil‐based fuels. The bioethanol industry needs to be more competitive, and therefore, all stages of its production process must be simple, inexpensi...

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Veröffentlicht in:Biotechnology progress 2007-11, Vol.23 (6), p.1454-1462
Hauptverfasser: Ochoa, S, Yoo, A, Repke, J.U, Wozny, G, Yang, D.R
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container_issue 6
container_start_page 1454
container_title Biotechnology progress
container_volume 23
creator Ochoa, S
Yoo, A
Repke, J.U
Wozny, G
Yang, D.R
description Despite many environmental advantages of using alcohol as a fuel, there are still serious questions about its economical feasibility when compared with oil‐based fuels. The bioethanol industry needs to be more competitive, and therefore, all stages of its production process must be simple, inexpensive, efficient, and “easy” to control. In recent years, there have been significant improvements in process design, such as in the purification technologies for ethanol dehydration (molecular sieves, pressure swing adsorption, pervaporation, etc.) and in genetic modifications of microbial strains. However, a lot of research effort is still required in optimization and control, where the first step is the development of suitable models of the process, which can be used as a simulated plant, as a soft sensor or as part of the control algorithm. Thus, toward developing good, reliable, and simple but highly predictive models that can be used in the future for optimization and process control applications, in this paper an unstructured and a cybernetic model are proposed and compared for the simultaneous saccharification‐fermentation process (SSF) for the production of ethanol from starch by a recombinant Saccharomyces cerevisiae strain. The cybernetic model proposed is a new one that considers the degradation of starch not only into glucose but also into dextrins (reducing sugars) and takes into account the intracellular reactions occurring inside the cells, giving a more detailed description of the process. Furthermore, an identification procedure based on the Metropolis Monte Carlo optimization method coupled with a sensitivity analysis is proposed for the identification of the modelapos;s parameters, employing experimental data reported in the literature.
doi_str_mv 10.1021/bp0702119
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source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects alcoholic fermentation
alpha-amylase
Aspergillus awamori
Bacillus subtilis
Biological and medical sciences
Biotechnology
Carbohydrate Metabolism
dextrins
Energy-Generating Resources
enzymatic hydrolysis
ethanol
Ethanol - metabolism
ethanol production
Fermentation
fuels
Fundamental and applied biological sciences. Psychology
genetically engineered microorganisms
glucan 1,4-alpha-glucosidase
glucose
mathematical models
Monte Carlo Method
optimization
saccharification
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
simulation models
simultaneous saccharification-fermentation
starch
Starch - metabolism
yeasts
title Modeling and Parameter Identification of the Simultaneous Saccharification-Fermentation Process for Ethanol Production
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