Modeling and Parameter Identification of the Simultaneous Saccharification-Fermentation Process for Ethanol Production
Despite many environmental advantages of using alcohol as a fuel, there are still serious questions about its economical feasibility when compared with oil‐based fuels. The bioethanol industry needs to be more competitive, and therefore, all stages of its production process must be simple, inexpensi...
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Veröffentlicht in: | Biotechnology progress 2007-11, Vol.23 (6), p.1454-1462 |
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description | Despite many environmental advantages of using alcohol as a fuel, there are still serious questions about its economical feasibility when compared with oil‐based fuels. The bioethanol industry needs to be more competitive, and therefore, all stages of its production process must be simple, inexpensive, efficient, and “easy” to control. In recent years, there have been significant improvements in process design, such as in the purification technologies for ethanol dehydration (molecular sieves, pressure swing adsorption, pervaporation, etc.) and in genetic modifications of microbial strains. However, a lot of research effort is still required in optimization and control, where the first step is the development of suitable models of the process, which can be used as a simulated plant, as a soft sensor or as part of the control algorithm. Thus, toward developing good, reliable, and simple but highly predictive models that can be used in the future for optimization and process control applications, in this paper an unstructured and a cybernetic model are proposed and compared for the simultaneous saccharification‐fermentation process (SSF) for the production of ethanol from starch by a recombinant Saccharomyces cerevisiae strain. The cybernetic model proposed is a new one that considers the degradation of starch not only into glucose but also into dextrins (reducing sugars) and takes into account the intracellular reactions occurring inside the cells, giving a more detailed description of the process. Furthermore, an identification procedure based on the Metropolis Monte Carlo optimization method coupled with a sensitivity analysis is proposed for the identification of the modelapos;s parameters, employing experimental data reported in the literature. |
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The bioethanol industry needs to be more competitive, and therefore, all stages of its production process must be simple, inexpensive, efficient, and “easy” to control. In recent years, there have been significant improvements in process design, such as in the purification technologies for ethanol dehydration (molecular sieves, pressure swing adsorption, pervaporation, etc.) and in genetic modifications of microbial strains. However, a lot of research effort is still required in optimization and control, where the first step is the development of suitable models of the process, which can be used as a simulated plant, as a soft sensor or as part of the control algorithm. Thus, toward developing good, reliable, and simple but highly predictive models that can be used in the future for optimization and process control applications, in this paper an unstructured and a cybernetic model are proposed and compared for the simultaneous saccharification‐fermentation process (SSF) for the production of ethanol from starch by a recombinant Saccharomyces cerevisiae strain. The cybernetic model proposed is a new one that considers the degradation of starch not only into glucose but also into dextrins (reducing sugars) and takes into account the intracellular reactions occurring inside the cells, giving a more detailed description of the process. Furthermore, an identification procedure based on the Metropolis Monte Carlo optimization method coupled with a sensitivity analysis is proposed for the identification of the modelapos;s parameters, employing experimental data reported in the literature.</description><identifier>ISSN: 8756-7938</identifier><identifier>EISSN: 1520-6033</identifier><identifier>DOI: 10.1021/bp0702119</identifier><identifier>PMID: 17935346</identifier><identifier>CODEN: BIPRET</identifier><language>eng</language><publisher>USA: American Chemical Society</publisher><subject>alcoholic fermentation ; alpha-amylase ; Aspergillus awamori ; Bacillus subtilis ; Biological and medical sciences ; Biotechnology ; Carbohydrate Metabolism ; dextrins ; Energy-Generating Resources ; enzymatic hydrolysis ; ethanol ; Ethanol - metabolism ; ethanol production ; Fermentation ; fuels ; Fundamental and applied biological sciences. Psychology ; genetically engineered microorganisms ; glucan 1,4-alpha-glucosidase ; glucose ; mathematical models ; Monte Carlo Method ; optimization ; saccharification ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - metabolism ; simulation models ; simultaneous saccharification-fermentation ; starch ; Starch - metabolism ; yeasts</subject><ispartof>Biotechnology progress, 2007-11, Vol.23 (6), p.1454-1462</ispartof><rights>Copyright © 2007 American Institute of Chemical Engineers (AIChE)</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4479-d245070cbb46028211a759336b45ff5e9f68a0a7a2eda4cfeca2c3325bf48953</citedby><cites>FETCH-LOGICAL-c4479-d245070cbb46028211a759336b45ff5e9f68a0a7a2eda4cfeca2c3325bf48953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1021%2Fbp0702119$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1021%2Fbp0702119$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19919424$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17935346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ochoa, S</creatorcontrib><creatorcontrib>Yoo, A</creatorcontrib><creatorcontrib>Repke, J.U</creatorcontrib><creatorcontrib>Wozny, G</creatorcontrib><creatorcontrib>Yang, D.R</creatorcontrib><title>Modeling and Parameter Identification of the Simultaneous Saccharification-Fermentation Process for Ethanol Production</title><title>Biotechnology progress</title><addtitle>Biotechnol Prog</addtitle><description>Despite many environmental advantages of using alcohol as a fuel, there are still serious questions about its economical feasibility when compared with oil‐based fuels. The bioethanol industry needs to be more competitive, and therefore, all stages of its production process must be simple, inexpensive, efficient, and “easy” to control. In recent years, there have been significant improvements in process design, such as in the purification technologies for ethanol dehydration (molecular sieves, pressure swing adsorption, pervaporation, etc.) and in genetic modifications of microbial strains. However, a lot of research effort is still required in optimization and control, where the first step is the development of suitable models of the process, which can be used as a simulated plant, as a soft sensor or as part of the control algorithm. Thus, toward developing good, reliable, and simple but highly predictive models that can be used in the future for optimization and process control applications, in this paper an unstructured and a cybernetic model are proposed and compared for the simultaneous saccharification‐fermentation process (SSF) for the production of ethanol from starch by a recombinant Saccharomyces cerevisiae strain. The cybernetic model proposed is a new one that considers the degradation of starch not only into glucose but also into dextrins (reducing sugars) and takes into account the intracellular reactions occurring inside the cells, giving a more detailed description of the process. Furthermore, an identification procedure based on the Metropolis Monte Carlo optimization method coupled with a sensitivity analysis is proposed for the identification of the modelapos;s parameters, employing experimental data reported in the literature.</description><subject>alcoholic fermentation</subject><subject>alpha-amylase</subject><subject>Aspergillus awamori</subject><subject>Bacillus subtilis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carbohydrate Metabolism</subject><subject>dextrins</subject><subject>Energy-Generating Resources</subject><subject>enzymatic hydrolysis</subject><subject>ethanol</subject><subject>Ethanol - metabolism</subject><subject>ethanol production</subject><subject>Fermentation</subject><subject>fuels</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetically engineered microorganisms</subject><subject>glucan 1,4-alpha-glucosidase</subject><subject>glucose</subject><subject>mathematical models</subject><subject>Monte Carlo Method</subject><subject>optimization</subject><subject>saccharification</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>simulation models</subject><subject>simultaneous saccharification-fermentation</subject><subject>starch</subject><subject>Starch - metabolism</subject><subject>yeasts</subject><issn>8756-7938</issn><issn>1520-6033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9P3DAQxS3UChbKgS_Q-lIkDmn9P_GxRVCQqLrqbs_RxLFZV0m8tRMQ375eZbWcqp5GGv_eG70ZI3RBySdKGP3cbEmZK9VHaEElI4UinL9Bi6qUqig1r07QaUq_CSEVUewYndDclFyoBXr6Hlrb-eERw9DiJUTo7Wgjvm_tMHrnDYw-DDg4PG4sXvl-6kYYbJgSXoExG4gHqLi1sc-qWbGMwdiUsAsR34wbGEK367WT2T2_Q28ddMme7-sZWt_erK_viocf3-6vvzwURohSFy0TMkczTSMUYVWOCKXUnKtGSOek1U5VQKAEZlsQxlkDzHDOZONEpSU_Q5ez7TaGP5NNY937ZGzXzRFqpYlglVL_BRkRUpGSZ_BqBk0MKUXr6m30PcSXmpJ6d4z6cIzMvt-bTk1v21dyv_0MfNwDkAx0LsJgfHrltKZaMJE5OnPPvrMv_55Yf10vfx6Gf5g1DkINjzH7_loxQnn-BIopxvhfFtqqzw</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Ochoa, S</creator><creator>Yoo, A</creator><creator>Repke, J.U</creator><creator>Wozny, G</creator><creator>Yang, D.R</creator><general>American Chemical Society</general><general>American Institute of Chemical Engineers</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Modeling and Parameter Identification of the Simultaneous Saccharification-Fermentation Process for Ethanol Production</title><author>Ochoa, S ; Yoo, A ; Repke, J.U ; Wozny, G ; Yang, D.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4479-d245070cbb46028211a759336b45ff5e9f68a0a7a2eda4cfeca2c3325bf48953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>alcoholic fermentation</topic><topic>alpha-amylase</topic><topic>Aspergillus awamori</topic><topic>Bacillus subtilis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carbohydrate Metabolism</topic><topic>dextrins</topic><topic>Energy-Generating Resources</topic><topic>enzymatic hydrolysis</topic><topic>ethanol</topic><topic>Ethanol - metabolism</topic><topic>ethanol production</topic><topic>Fermentation</topic><topic>fuels</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetically engineered microorganisms</topic><topic>glucan 1,4-alpha-glucosidase</topic><topic>glucose</topic><topic>mathematical models</topic><topic>Monte Carlo Method</topic><topic>optimization</topic><topic>saccharification</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>simulation models</topic><topic>simultaneous saccharification-fermentation</topic><topic>starch</topic><topic>Starch - metabolism</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ochoa, S</creatorcontrib><creatorcontrib>Yoo, A</creatorcontrib><creatorcontrib>Repke, J.U</creatorcontrib><creatorcontrib>Wozny, G</creatorcontrib><creatorcontrib>Yang, D.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology progress</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ochoa, S</au><au>Yoo, A</au><au>Repke, J.U</au><au>Wozny, G</au><au>Yang, D.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modeling and Parameter Identification of the Simultaneous Saccharification-Fermentation Process for Ethanol Production</atitle><jtitle>Biotechnology progress</jtitle><addtitle>Biotechnol Prog</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>23</volume><issue>6</issue><spage>1454</spage><epage>1462</epage><pages>1454-1462</pages><issn>8756-7938</issn><eissn>1520-6033</eissn><coden>BIPRET</coden><abstract>Despite many environmental advantages of using alcohol as a fuel, there are still serious questions about its economical feasibility when compared with oil‐based fuels. The bioethanol industry needs to be more competitive, and therefore, all stages of its production process must be simple, inexpensive, efficient, and “easy” to control. In recent years, there have been significant improvements in process design, such as in the purification technologies for ethanol dehydration (molecular sieves, pressure swing adsorption, pervaporation, etc.) and in genetic modifications of microbial strains. However, a lot of research effort is still required in optimization and control, where the first step is the development of suitable models of the process, which can be used as a simulated plant, as a soft sensor or as part of the control algorithm. Thus, toward developing good, reliable, and simple but highly predictive models that can be used in the future for optimization and process control applications, in this paper an unstructured and a cybernetic model are proposed and compared for the simultaneous saccharification‐fermentation process (SSF) for the production of ethanol from starch by a recombinant Saccharomyces cerevisiae strain. The cybernetic model proposed is a new one that considers the degradation of starch not only into glucose but also into dextrins (reducing sugars) and takes into account the intracellular reactions occurring inside the cells, giving a more detailed description of the process. Furthermore, an identification procedure based on the Metropolis Monte Carlo optimization method coupled with a sensitivity analysis is proposed for the identification of the modelapos;s parameters, employing experimental data reported in the literature.</abstract><cop>USA</cop><pub>American Chemical Society</pub><pmid>17935346</pmid><doi>10.1021/bp0702119</doi><tpages>9</tpages></addata></record> |
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subjects | alcoholic fermentation alpha-amylase Aspergillus awamori Bacillus subtilis Biological and medical sciences Biotechnology Carbohydrate Metabolism dextrins Energy-Generating Resources enzymatic hydrolysis ethanol Ethanol - metabolism ethanol production Fermentation fuels Fundamental and applied biological sciences. Psychology genetically engineered microorganisms glucan 1,4-alpha-glucosidase glucose mathematical models Monte Carlo Method optimization saccharification Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism simulation models simultaneous saccharification-fermentation starch Starch - metabolism yeasts |
title | Modeling and Parameter Identification of the Simultaneous Saccharification-Fermentation Process for Ethanol Production |
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