Termination Factor-Mediated DNA Loop between Termination and Initiation Sites Drives Mitochondrial rRNA Synthesis

The human mitochondrial transcription termination factor mTERF plays a central role in the control of heavy-strand rDNA transcription by promoting initiation, besides termination, of this transcription. However, until now, the mechanism underlying this stimulation of transcription by mTERF was not u...

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Veröffentlicht in:Cell 2005-12, Vol.123 (7), p.1227-1240
Hauptverfasser: Martin, Miguel, Cho, Jaehyoung, Cesare, Anthony J., Griffith, Jack D., Attardi, Giuseppe
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container_end_page 1240
container_issue 7
container_start_page 1227
container_title Cell
container_volume 123
creator Martin, Miguel
Cho, Jaehyoung
Cesare, Anthony J.
Griffith, Jack D.
Attardi, Giuseppe
description The human mitochondrial transcription termination factor mTERF plays a central role in the control of heavy-strand rDNA transcription by promoting initiation, besides termination, of this transcription. However, until now, the mechanism underlying this stimulation of transcription by mTERF was not understood. In the present work, addition of mTERF to a HeLa cell mitochondrial lysate-based reaction mixture containing an artificial rDNA template did indeed specifically stimulate rDNA transcription. This stimulation required that mTERF be simultaneously bound to the rDNA transcription termination and initiation sites in the same molecule, thus forming a loop. Most significantly, a double binding of mTERF to the rDNA molecule, with resulting loop formation, was also shown in vivo. These results strongly suggest that, to satisfy the need for high rate of rDNA transcription, human mitochondrial rRNA synthesis involves mTERF-mediated rDNA looping that promotes recycling of the transcription machinery.
doi_str_mv 10.1016/j.cell.2005.09.040
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subjects Cell-Free System - chemistry
Cell-Free System - metabolism
DNA - chemistry
HeLa Cells
Humans
Mitochondria - chemistry
RNA - biosynthesis
RNA - genetics
RNA, Ribosomal - biosynthesis
Telomeric Repeat Binding Protein 1 - genetics
Telomeric Repeat Binding Protein 1 - metabolism
Terminator Regions, Genetic - physiology
Transcription Initiation Site - physiology
Transcription, Genetic - physiology
title Termination Factor-Mediated DNA Loop between Termination and Initiation Sites Drives Mitochondrial rRNA Synthesis
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