Determination of saponins in Maesa lanceolata by LC-UV: Development and validation

A method is described to quantify the saponins in Maesa lanceolata extracts. The method is based on a H 2O/MeOH extraction and a purification step on a C 18 SPE cartridge. The identification is done by the combination of LC-UV/MS, while quantification is performed by LC-UV, using oleanolic acid as a...

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Veröffentlicht in:	Phytochemistry 2007-11, Vol.68 (22), p.2825-2830
Hauptverfasser:	Theunis, Mart H.B.L., Foubert, Kenn, Pollier, Jacob, Gonzalez-Guzman, Miguel, Goossens, Alain, Vlietinck, Arnold J., Pieters, Luc A.C., Apers, Sandra
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Biological and medical sciences Calibration Chemical constitution Chromatography, Liquid - methods Fundamental and applied biological sciences. Psychology General pharmacology HPLC liquid chromatography Maesa Maesa lanceolata Mass spectrometry Medical sciences medicinal plants Method development Method validation Models, Biological Molecular Structure Myrsinaceae Myrsinaceae - chemistry Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments plant biochemistry Plant Leaves - chemistry Plant physiology and development Plant Roots - chemistry Saponin saponins Saponins - chemistry Sensitivity and Specificity Spectrophotometry - methods triterpenoid saponins ultraviolet-visible spectroscopy
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