Toxoplasma gondii: localization of purine nucleoside phosphorylase activity in vitro and in vivo by electron microscopy

Purine nucleoside phosphorylase (PNP, EC 2.4.2.1) activity was revealed by enzyme histochemistry in Toxoplasma gondii ME49 strain isolated from murine cerebral cysts and from in vitro cultivation. The activity of the enzyme was revealed by an insoluble electron-opaque precipitate of lead phosphate a...

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Veröffentlicht in:	Medical molecular morphology 2005-12, Vol.38 (4), p.251-255
Hauptverfasser:	Gherardi, Arnaud, Peyrol, Simone, Sarciron, Marie-Elisabeth
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological Transport Brain - ultrastructure Cell Line, Tumor Cell Membrane - ultrastructure Cell Nucleus - ultrastructure Cysts Cysts - ultrastructure Cytoplasm - ultrastructure Mice Microscopy, Electron Purine-Nucleoside Phosphorylase - analysis Purine-Nucleoside Phosphorylase - metabolism Purine-Nucleoside Phosphorylase - ultrastructure Toxoplasma - enzymology Toxoplasma gondii
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