Survival and death of mature avian motoneurons in organotypic slice culture: Trophic requirements for survival and different types of degeneration

We have developed an organotypic culture technique that uses slices of chick embryo spinal cord, in which trophic requirements for long‐term survival of mature motoneurons (MNs) were studied. Slices were obtained from E16 chick embryos and maintained for up to 28 days in vitro (DIV) in a basal mediu...

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Veröffentlicht in:	Journal of comparative neurology (1911) 2007-04, Vol.501 (5), p.669-690
Hauptverfasser:	Brunet, Núria, Tarabal, Olga, Portero-Otín, Manel, Oppenheim, Ronald W., Esquerda, Josep E., Calderó, Jordi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis - drug effects Apoptosis - physiology Autophagy - drug effects Autophagy - physiology Caspase 3 - metabolism Caspase Inhibitors cell death Cell Survival - drug effects Cell Survival - physiology Chick Embryo Cyclic AMP - metabolism Glial Cell Line-Derived Neurotrophic Factor - metabolism Glial Cell Line-Derived Neurotrophic Factor - pharmacology Gliosis - drug therapy Gliosis - metabolism Gliosis - physiopathology Immunohistochemistry In Situ Nick-End Labeling Membrane Potentials - drug effects Membrane Potentials - physiology Microscopy, Electron, Transmission motoneurons Motor Neurons - drug effects Motor Neurons - metabolism Motor Neurons - pathology Nerve Degeneration - drug therapy Nerve Degeneration - metabolism Nerve Degeneration - pathology Nerve Growth Factors - metabolism Nerve Growth Factors - pharmacology neurotrophic factors Organ Culture Techniques organotypic slice culture Potassium - metabolism Potassium - pharmacology spinal cord Spinal Cord - embryology Spinal Cord - metabolism Spinal Cord - pathology Vascular Endothelial Growth Factor A - metabolism Vascular Endothelial Growth Factor A - pharmacology
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