Age-related Differences in Expression of Vascular Endothelial Growth Factor by Periodontal Ligament Cells In Vitro
The purpose of this study was to evaluate age-related differences in expression of vascular endothelial growth factor (VEGF) by periodontal ligament (PDL) cells. PDL cells were obtained from Wistar male rats weighing approximately 150g each in the young group and 350g each in the old group. PDL cell...
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| Veröffentlicht in: | Bulletin of Tokyo Dental College 2007, Vol.48(3), pp.143-146 |
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| creator | Matsuzaka, Kenichi Tsuruoka, Morito Kokubu, Eitoyo Katakura, Akira Endo, Takayuki Shibukawa, Yoshihiro Shintani, Masuro Tazaki, Masakazu Ishihara, Kazuyuki Hashimoto, Sadamitsu Yoshinari, Masao Inoue, Takashi |
| description | The purpose of this study was to evaluate age-related differences in expression of vascular endothelial growth factor (VEGF) by periodontal ligament (PDL) cells. PDL cells were obtained from Wistar male rats weighing approximately 150g each in the young group and 350g each in the old group. PDL cells derived from upper and lower incisors were seeded in 35-mm culture dishes after primary culture. For cell proliferation assays, cells were detached and counted at 1, 3, 5, 7, 11 and 14 days after culture. VEGF mRNA expression was analyzed with TaqMan ® . The number of cells in both groups increased day by day, but the rate of increase in the young group was higher than that in the old group. VEGF mRNA expression in the young group increased from 3 to 14 days, but in the old group increased only slightly over the same time period. Expression ratios in the young group were higher than those in the old group, and there were significant differences between the young and old groups at 7 and 14 days of culture. In conclusion, the data revealed that PDL cells varied with age, and suggest that in view of such changes in cell proliferation and VEGF mRNA expression, age should be taken into consideration in periodontal treatment. |
| doi_str_mv | 10.2209/tdcpublication.48.143 |
| format | Article |
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PDL cells were obtained from Wistar male rats weighing approximately 150g each in the young group and 350g each in the old group. PDL cells derived from upper and lower incisors were seeded in 35-mm culture dishes after primary culture. For cell proliferation assays, cells were detached and counted at 1, 3, 5, 7, 11 and 14 days after culture. VEGF mRNA expression was analyzed with TaqMan ® . The number of cells in both groups increased day by day, but the rate of increase in the young group was higher than that in the old group. VEGF mRNA expression in the young group increased from 3 to 14 days, but in the old group increased only slightly over the same time period. Expression ratios in the young group were higher than those in the old group, and there were significant differences between the young and old groups at 7 and 14 days of culture. In conclusion, the data revealed that PDL cells varied with age, and suggest that in view of such changes in cell proliferation and VEGF mRNA expression, age should be taken into consideration in periodontal treatment.</description><identifier>ISSN: 0040-8891</identifier><identifier>DOI: 10.2209/tdcpublication.48.143</identifier><identifier>PMID: 18057861</identifier><language>eng</language><publisher>Japan: Tokyo Dental College, Japan</publisher><subject>Age Factors ; Aging ; Animals ; Cell Proliferation ; Dentistry ; In vitro ; Male ; Periodontal ligament ; Periodontal Ligament - cytology ; Rats ; Rats, Wistar ; RNA, Messenger - analysis ; Vascular Endothelial Growth Factor A - analysis ; Vascular Endothelial Growth Factor A - biosynthesis ; VEGF</subject><ispartof>The Bulletin of Tokyo Dental College, 2007, Vol.48(3), pp.143-146</ispartof><rights>2007 by Tokyo Dental College, Japan</rights><rights>Copyright Japan Science and Technology Agency 2007</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5553-3a7243498c3de7c358bc2bc03e4268d1af55307ca74e2770135a9c70198e71743</citedby><cites>FETCH-LOGICAL-c5553-3a7243498c3de7c358bc2bc03e4268d1af55307ca74e2770135a9c70198e71743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18057861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuzaka, Kenichi</creatorcontrib><creatorcontrib>Tsuruoka, Morito</creatorcontrib><creatorcontrib>Kokubu, Eitoyo</creatorcontrib><creatorcontrib>Katakura, Akira</creatorcontrib><creatorcontrib>Endo, Takayuki</creatorcontrib><creatorcontrib>Shibukawa, Yoshihiro</creatorcontrib><creatorcontrib>Shintani, Masuro</creatorcontrib><creatorcontrib>Tazaki, Masakazu</creatorcontrib><creatorcontrib>Ishihara, Kazuyuki</creatorcontrib><creatorcontrib>Hashimoto, Sadamitsu</creatorcontrib><creatorcontrib>Yoshinari, Masao</creatorcontrib><creatorcontrib>Inoue, Takashi</creatorcontrib><title>Age-related Differences in Expression of Vascular Endothelial Growth Factor by Periodontal Ligament Cells In Vitro</title><title>Bulletin of Tokyo Dental College</title><addtitle>Bull. Tokyo Dent. Coll.</addtitle><description>The purpose of this study was to evaluate age-related differences in expression of vascular endothelial growth factor (VEGF) by periodontal ligament (PDL) cells. PDL cells were obtained from Wistar male rats weighing approximately 150g each in the young group and 350g each in the old group. PDL cells derived from upper and lower incisors were seeded in 35-mm culture dishes after primary culture. For cell proliferation assays, cells were detached and counted at 1, 3, 5, 7, 11 and 14 days after culture. VEGF mRNA expression was analyzed with TaqMan ® . The number of cells in both groups increased day by day, but the rate of increase in the young group was higher than that in the old group. VEGF mRNA expression in the young group increased from 3 to 14 days, but in the old group increased only slightly over the same time period. Expression ratios in the young group were higher than those in the old group, and there were significant differences between the young and old groups at 7 and 14 days of culture. In conclusion, the data revealed that PDL cells varied with age, and suggest that in view of such changes in cell proliferation and VEGF mRNA expression, age should be taken into consideration in periodontal treatment.</description><subject>Age Factors</subject><subject>Aging</subject><subject>Animals</subject><subject>Cell Proliferation</subject><subject>Dentistry</subject><subject>In vitro</subject><subject>Male</subject><subject>Periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - analysis</subject><subject>Vascular Endothelial Growth Factor A - analysis</subject><subject>Vascular Endothelial Growth Factor A - biosynthesis</subject><subject>VEGF</subject><issn>0040-8891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkcFuEzEQhn0A0VJ4BJAlJG4b7LU39h6rkJaiSHCAXi2vdzZx5Nhh7FXp22OUqAjExXPw938azU_IG84Wbcv6D2V0x3kI3tniU1xIveBSPCOXjEnWaN3zC_Iy5z1jQnMtX5ALrlmn9JJfErzeQoMQbIGRfvTTBAjRQaY-0vXPI0LOVUnTRO9tdnOwSNdxTGUHwdtAbzE9lB29sa4kpMMj_Qro05hiqZ8bv7UHiIWuIIRM7yK99wXTK_J8siHD6_O8It9v1t9Wn5rNl9u71fWmcV3XiUZY1Uohe-3ECMqJTg-uHRwTINulHrmdKsWUs0pCqxTjorO9q7PXoLiS4oq8P3mPmH7MkIs5-OzqKjZCmrNZ9kxIKVkF3_0D7tOMse5muFzW80nd95XqTpTDlDPCZI7oDxYfDWfmdw3m7xqM1DUuau7t2T4PBxj_pM4dVODzCdjnYrfwBFgs3gX4j1acnmp_gtzOooEofgEnZaVK</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Matsuzaka, Kenichi</creator><creator>Tsuruoka, Morito</creator><creator>Kokubu, Eitoyo</creator><creator>Katakura, Akira</creator><creator>Endo, Takayuki</creator><creator>Shibukawa, Yoshihiro</creator><creator>Shintani, Masuro</creator><creator>Tazaki, Masakazu</creator><creator>Ishihara, Kazuyuki</creator><creator>Hashimoto, Sadamitsu</creator><creator>Yoshinari, Masao</creator><creator>Inoue, Takashi</creator><general>Tokyo Dental College, Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>2007</creationdate><title>Age-related Differences in Expression of Vascular Endothelial Growth Factor by Periodontal Ligament Cells In Vitro</title><author>Matsuzaka, Kenichi ; Tsuruoka, Morito ; Kokubu, Eitoyo ; Katakura, Akira ; Endo, Takayuki ; Shibukawa, Yoshihiro ; Shintani, Masuro ; Tazaki, Masakazu ; Ishihara, Kazuyuki ; Hashimoto, Sadamitsu ; Yoshinari, Masao ; Inoue, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5553-3a7243498c3de7c358bc2bc03e4268d1af55307ca74e2770135a9c70198e71743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Age Factors</topic><topic>Aging</topic><topic>Animals</topic><topic>Cell Proliferation</topic><topic>Dentistry</topic><topic>In vitro</topic><topic>Male</topic><topic>Periodontal ligament</topic><topic>Periodontal Ligament - cytology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - analysis</topic><topic>Vascular Endothelial Growth Factor A - analysis</topic><topic>Vascular Endothelial Growth Factor A - biosynthesis</topic><topic>VEGF</topic><toplevel>online_resources</toplevel><creatorcontrib>Matsuzaka, Kenichi</creatorcontrib><creatorcontrib>Tsuruoka, Morito</creatorcontrib><creatorcontrib>Kokubu, Eitoyo</creatorcontrib><creatorcontrib>Katakura, Akira</creatorcontrib><creatorcontrib>Endo, Takayuki</creatorcontrib><creatorcontrib>Shibukawa, Yoshihiro</creatorcontrib><creatorcontrib>Shintani, Masuro</creatorcontrib><creatorcontrib>Tazaki, Masakazu</creatorcontrib><creatorcontrib>Ishihara, Kazuyuki</creatorcontrib><creatorcontrib>Hashimoto, Sadamitsu</creatorcontrib><creatorcontrib>Yoshinari, Masao</creatorcontrib><creatorcontrib>Inoue, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Bulletin of Tokyo Dental College</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuzaka, Kenichi</au><au>Tsuruoka, Morito</au><au>Kokubu, Eitoyo</au><au>Katakura, Akira</au><au>Endo, Takayuki</au><au>Shibukawa, Yoshihiro</au><au>Shintani, Masuro</au><au>Tazaki, Masakazu</au><au>Ishihara, Kazuyuki</au><au>Hashimoto, Sadamitsu</au><au>Yoshinari, Masao</au><au>Inoue, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-related Differences in Expression of Vascular Endothelial Growth Factor by Periodontal Ligament Cells In Vitro</atitle><jtitle>Bulletin of Tokyo Dental College</jtitle><addtitle>Bull. Tokyo Dent. Coll.</addtitle><date>2007</date><risdate>2007</risdate><volume>48</volume><issue>3</issue><spage>143</spage><epage>146</epage><pages>143-146</pages><issn>0040-8891</issn><abstract>The purpose of this study was to evaluate age-related differences in expression of vascular endothelial growth factor (VEGF) by periodontal ligament (PDL) cells. PDL cells were obtained from Wistar male rats weighing approximately 150g each in the young group and 350g each in the old group. PDL cells derived from upper and lower incisors were seeded in 35-mm culture dishes after primary culture. For cell proliferation assays, cells were detached and counted at 1, 3, 5, 7, 11 and 14 days after culture. VEGF mRNA expression was analyzed with TaqMan ® . The number of cells in both groups increased day by day, but the rate of increase in the young group was higher than that in the old group. VEGF mRNA expression in the young group increased from 3 to 14 days, but in the old group increased only slightly over the same time period. Expression ratios in the young group were higher than those in the old group, and there were significant differences between the young and old groups at 7 and 14 days of culture. In conclusion, the data revealed that PDL cells varied with age, and suggest that in view of such changes in cell proliferation and VEGF mRNA expression, age should be taken into consideration in periodontal treatment.</abstract><cop>Japan</cop><pub>Tokyo Dental College, Japan</pub><pmid>18057861</pmid><doi>10.2209/tdcpublication.48.143</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Age Factors Aging Animals Cell Proliferation Dentistry In vitro Male Periodontal ligament Periodontal Ligament - cytology Rats Rats, Wistar RNA, Messenger - analysis Vascular Endothelial Growth Factor A - analysis Vascular Endothelial Growth Factor A - biosynthesis VEGF |
| title | Age-related Differences in Expression of Vascular Endothelial Growth Factor by Periodontal Ligament Cells In Vitro |
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