Quantitative proteome analysis of cisplatin-induced apoptotic Jurkat T cells by stable isotope labeling with amino acids in cell culture, SDS-PAGE, and LC-MALDI-TOF/TOF MS

Quantitative proteome analysis of cisplatin-induced apoptosis in total Jurkat T cell lysates was performed in order to identify modified proteins. Proteins were labeled in cell culture with stable isotopes of arginines, and fractionated by SDS-PAGE. Subsequently, tryptic peptides were analyzed by na...

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Veröffentlicht in:	Electrophoresis 2007-12, Vol.28 (23), p.4359-4368
Hauptverfasser:	Schmidt, Frank, Hustoft, Hanne K, Strozynski, Margarita, Dimmler, Christiane, Rudel, Thomas, Thiede, Bernd
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - chemistry Amino Acids - metabolism Apoptosis Apoptosis - genetics Arginine - chemistry Arginine - metabolism Caspase 3 - metabolism Cell Culture Techniques Chromatography, High Pressure Liquid - methods Cisplatin Cisplatin - pharmacology Core Binding Factor beta Subunit - metabolism Electrophoresis, Gel, Two-Dimensional - methods Electrophoresis, Polyacrylamide Gel - methods Humans Isotope Labeling - methods Jurkat Cells - metabolism LC-MALDI Methionine - chemistry Methionine - metabolism Nuclear Matrix-Associated Proteins - metabolism Octamer Transcription Factors - metabolism p54nrb Peptide Elongation Factor 2 - metabolism Peptide Mapping Proteome - metabolism RNA-Binding Proteins - metabolism Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Stable isotope labeling with amino acids in cell culture
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