Liquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile
A liquid chromatography method for simultaneous analysis of amino acids, polyamines, catecholeamines and metanephrines in human body fluids after derivatization with 9-fluorenylmethyloxycarbonyl chloride was developed. The chromatographic behavior of analytes at different pH of mobile phase was stud...
Gespeichert in:
| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-12, Vol.860 (1), p.92-97 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 97 |
|---|---|
| container_issue | 1 |
| container_start_page | 92 |
| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| container_volume | 860 |
| creator | Lozanov, Valentin Benkova, Bistra Mateva, Lyudmila Petrov, Stefan Popov, Elenko Slavov, Chavdar Mitev, Vanio |
| description | A liquid chromatography method for simultaneous analysis of amino acids, polyamines, catecholeamines and metanephrines in human body fluids after derivatization with 9-fluorenylmethyloxycarbonyl chloride was developed. The chromatographic behavior of analytes at different pH of mobile phase was studied. Successful baseline resolution of all analyzed compounds was achieved using simultaneous gradient of pH and organic modifier in reverse phase mode of HPLC within 36
min. The repeatability of the proposed procedure in respect of retention time and peak area, expressed as RSD, ranges from 0.06 to 1.64% and 0.4 to 7.6%, respectively. The method linearity in the range of 1–200
μM for amino acids and in the range of 0.1–20
μM for polyamines, catecholeamines and metanephrines was found to be with correlation coefficients higher than 0.994. The limit of quantification (LOQ) was assessed to be in the range of 2.6–10
pmol for amino acids and 2–4
pmol for polyamines, catecholeamines and metanephrines. |
| doi_str_mv | 10.1016/j.jchromb.2007.10.020 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69027127</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1570023207007301</els_id><sourcerecordid>69027127</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-116f672d5d24aa752d27d6f3221d76f665ff07e491ac1e134a147a9eb1b28be43</originalsourceid><addsrcrecordid>eNqFkc-KFDEQxhtR3HX1EZRc9NZjkv6T7pPI4u4KA14UvIV0UpmpId2ZTdLKvI2PanqmYfHkKeGrX31V1FcUbxndMMraj4fNQe-DH4cNp1RkbUM5fVZcs05UZSXan8_zvxG0pLziV8WrGA-UMkFF9bK4YqLvqq5tros_W3yc0ZCzl0p-F9RxfyIjpL03xPpAIo6zS2oCP0eiJuVOESPxlqgRJ0-URrPohgzodzChPhcgEpwWyfkdauWIdfMC_sa0_9cyTzQIU1osjw9nJ6Uh-QlTQAevixdWuQhv1vem-HH35fvtQ7n9dv_19vO21DXnqWSsta3gpjG8Vko03HBhWltxzozIpbaxlgqoe6Y0A1bVitVC9TCwgXcD1NVN8eHiewz-cYaY5IhRg3OXNWXbUy4YFxlsLqAOPsYAVh4DjiqcJKNyiUYe5BqNXKJZ5BxN7nu3DpiHEcxT15pFBt6vgIr5YjaoSWN84vouc7zK3KcLB_kcvxCCjDofUIPBADpJ4_E_q_wF5dyzgw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69027127</pqid></control><display><type>article</type><title>Liquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Lozanov, Valentin ; Benkova, Bistra ; Mateva, Lyudmila ; Petrov, Stefan ; Popov, Elenko ; Slavov, Chavdar ; Mitev, Vanio</creator><creatorcontrib>Lozanov, Valentin ; Benkova, Bistra ; Mateva, Lyudmila ; Petrov, Stefan ; Popov, Elenko ; Slavov, Chavdar ; Mitev, Vanio</creatorcontrib><description>A liquid chromatography method for simultaneous analysis of amino acids, polyamines, catecholeamines and metanephrines in human body fluids after derivatization with 9-fluorenylmethyloxycarbonyl chloride was developed. The chromatographic behavior of analytes at different pH of mobile phase was studied. Successful baseline resolution of all analyzed compounds was achieved using simultaneous gradient of pH and organic modifier in reverse phase mode of HPLC within 36
min. The repeatability of the proposed procedure in respect of retention time and peak area, expressed as RSD, ranges from 0.06 to 1.64% and 0.4 to 7.6%, respectively. The method linearity in the range of 1–200
μM for amino acids and in the range of 0.1–20
μM for polyamines, catecholeamines and metanephrines was found to be with correlation coefficients higher than 0.994. The limit of quantification (LOQ) was assessed to be in the range of 2.6–10
pmol for amino acids and 2–4
pmol for polyamines, catecholeamines and metanephrines.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2007.10.020</identifier><identifier>PMID: 17983865</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetonitriles ; Amino acids ; Amino Acids - blood ; Amino Acids - urine ; Analysis ; Analytical, structural and metabolic biochemistry ; Biogenic Amines - blood ; Biogenic Amines - urine ; Biological and medical sciences ; Catecholeamines ; Chromatography, High Pressure Liquid - methods ; Fluorenes - chemistry ; Fmoc ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Humans ; Hydrogen-Ion Concentration ; Liquid chromatography ; Medical sciences ; Metanephrines ; Pharmacology. Drug treatments ; Polyamines</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-12, Vol.860 (1), p.92-97</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-116f672d5d24aa752d27d6f3221d76f665ff07e491ac1e134a147a9eb1b28be43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2007.10.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19883823$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17983865$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lozanov, Valentin</creatorcontrib><creatorcontrib>Benkova, Bistra</creatorcontrib><creatorcontrib>Mateva, Lyudmila</creatorcontrib><creatorcontrib>Petrov, Stefan</creatorcontrib><creatorcontrib>Popov, Elenko</creatorcontrib><creatorcontrib>Slavov, Chavdar</creatorcontrib><creatorcontrib>Mitev, Vanio</creatorcontrib><title>Liquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A liquid chromatography method for simultaneous analysis of amino acids, polyamines, catecholeamines and metanephrines in human body fluids after derivatization with 9-fluorenylmethyloxycarbonyl chloride was developed. The chromatographic behavior of analytes at different pH of mobile phase was studied. Successful baseline resolution of all analyzed compounds was achieved using simultaneous gradient of pH and organic modifier in reverse phase mode of HPLC within 36
min. The repeatability of the proposed procedure in respect of retention time and peak area, expressed as RSD, ranges from 0.06 to 1.64% and 0.4 to 7.6%, respectively. The method linearity in the range of 1–200
μM for amino acids and in the range of 0.1–20
μM for polyamines, catecholeamines and metanephrines was found to be with correlation coefficients higher than 0.994. The limit of quantification (LOQ) was assessed to be in the range of 2.6–10
pmol for amino acids and 2–4
pmol for polyamines, catecholeamines and metanephrines.</description><subject>Acetonitriles</subject><subject>Amino acids</subject><subject>Amino Acids - blood</subject><subject>Amino Acids - urine</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biogenic Amines - blood</subject><subject>Biogenic Amines - urine</subject><subject>Biological and medical sciences</subject><subject>Catecholeamines</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fluorenes - chemistry</subject><subject>Fmoc</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Liquid chromatography</subject><subject>Medical sciences</subject><subject>Metanephrines</subject><subject>Pharmacology. Drug treatments</subject><subject>Polyamines</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-KFDEQxhtR3HX1EZRc9NZjkv6T7pPI4u4KA14UvIV0UpmpId2ZTdLKvI2PanqmYfHkKeGrX31V1FcUbxndMMraj4fNQe-DH4cNp1RkbUM5fVZcs05UZSXan8_zvxG0pLziV8WrGA-UMkFF9bK4YqLvqq5tros_W3yc0ZCzl0p-F9RxfyIjpL03xPpAIo6zS2oCP0eiJuVOESPxlqgRJ0-URrPohgzodzChPhcgEpwWyfkdauWIdfMC_sa0_9cyTzQIU1osjw9nJ6Uh-QlTQAevixdWuQhv1vem-HH35fvtQ7n9dv_19vO21DXnqWSsta3gpjG8Vko03HBhWltxzozIpbaxlgqoe6Y0A1bVitVC9TCwgXcD1NVN8eHiewz-cYaY5IhRg3OXNWXbUy4YFxlsLqAOPsYAVh4DjiqcJKNyiUYe5BqNXKJZ5BxN7nu3DpiHEcxT15pFBt6vgIr5YjaoSWN84vouc7zK3KcLB_kcvxCCjDofUIPBADpJ4_E_q_wF5dyzgw</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Lozanov, Valentin</creator><creator>Benkova, Bistra</creator><creator>Mateva, Lyudmila</creator><creator>Petrov, Stefan</creator><creator>Popov, Elenko</creator><creator>Slavov, Chavdar</creator><creator>Mitev, Vanio</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Liquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile</title><author>Lozanov, Valentin ; Benkova, Bistra ; Mateva, Lyudmila ; Petrov, Stefan ; Popov, Elenko ; Slavov, Chavdar ; Mitev, Vanio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-116f672d5d24aa752d27d6f3221d76f665ff07e491ac1e134a147a9eb1b28be43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acetonitriles</topic><topic>Amino acids</topic><topic>Amino Acids - blood</topic><topic>Amino Acids - urine</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biogenic Amines - blood</topic><topic>Biogenic Amines - urine</topic><topic>Biological and medical sciences</topic><topic>Catecholeamines</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fluorenes - chemistry</topic><topic>Fmoc</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Liquid chromatography</topic><topic>Medical sciences</topic><topic>Metanephrines</topic><topic>Pharmacology. Drug treatments</topic><topic>Polyamines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lozanov, Valentin</creatorcontrib><creatorcontrib>Benkova, Bistra</creatorcontrib><creatorcontrib>Mateva, Lyudmila</creatorcontrib><creatorcontrib>Petrov, Stefan</creatorcontrib><creatorcontrib>Popov, Elenko</creatorcontrib><creatorcontrib>Slavov, Chavdar</creatorcontrib><creatorcontrib>Mitev, Vanio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lozanov, Valentin</au><au>Benkova, Bistra</au><au>Mateva, Lyudmila</au><au>Petrov, Stefan</au><au>Popov, Elenko</au><au>Slavov, Chavdar</au><au>Mitev, Vanio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>860</volume><issue>1</issue><spage>92</spage><epage>97</epage><pages>92-97</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A liquid chromatography method for simultaneous analysis of amino acids, polyamines, catecholeamines and metanephrines in human body fluids after derivatization with 9-fluorenylmethyloxycarbonyl chloride was developed. The chromatographic behavior of analytes at different pH of mobile phase was studied. Successful baseline resolution of all analyzed compounds was achieved using simultaneous gradient of pH and organic modifier in reverse phase mode of HPLC within 36
min. The repeatability of the proposed procedure in respect of retention time and peak area, expressed as RSD, ranges from 0.06 to 1.64% and 0.4 to 7.6%, respectively. The method linearity in the range of 1–200
μM for amino acids and in the range of 0.1–20
μM for polyamines, catecholeamines and metanephrines was found to be with correlation coefficients higher than 0.994. The limit of quantification (LOQ) was assessed to be in the range of 2.6–10
pmol for amino acids and 2–4
pmol for polyamines, catecholeamines and metanephrines.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17983865</pmid><doi>10.1016/j.jchromb.2007.10.020</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-12, Vol.860 (1), p.92-97 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69027127 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Acetonitriles Amino acids Amino Acids - blood Amino Acids - urine Analysis Analytical, structural and metabolic biochemistry Biogenic Amines - blood Biogenic Amines - urine Biological and medical sciences Catecholeamines Chromatography, High Pressure Liquid - methods Fluorenes - chemistry Fmoc Fundamental and applied biological sciences. Psychology General pharmacology Humans Hydrogen-Ion Concentration Liquid chromatography Medical sciences Metanephrines Pharmacology. Drug treatments Polyamines |
| title | Liquid chromatography method for simultaneous analysis of amino acids and biogenic amines in biological fluids with simultaneous gradient of pH and acetonitrile |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T01%3A05%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Liquid%20chromatography%20method%20for%20simultaneous%20analysis%20of%20amino%20acids%20and%20biogenic%20amines%20in%20biological%20fluids%20with%20simultaneous%20gradient%20of%20pH%20and%20acetonitrile&rft.jtitle=Journal%20of%20chromatography.%20B,%20Analytical%20technologies%20in%20the%20biomedical%20and%20life%20sciences&rft.au=Lozanov,%20Valentin&rft.date=2007-12-01&rft.volume=860&rft.issue=1&rft.spage=92&rft.epage=97&rft.pages=92-97&rft.issn=1570-0232&rft.eissn=1873-376X&rft_id=info:doi/10.1016/j.jchromb.2007.10.020&rft_dat=%3Cproquest_cross%3E69027127%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69027127&rft_id=info:pmid/17983865&rft_els_id=S1570023207007301&rfr_iscdi=true |