Capillary Isoelectric Focusing — Useful tool for detection of the biofilm formation in Staphylococcus epidermidis
The biofilm formation is an important factor of S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The p...
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| Veröffentlicht in: | Journal of microbiological methods 2007-03, Vol.68 (3), p.530-535 |
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| container_title | Journal of microbiological methods |
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| creator | Ruzicka, Filip Horka, Marie Hola, Veronika Votava, Miroslav |
| description | The biofilm formation is an important factor of
S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The presence of this substance on the surface can affect physico-chemical properties of the bacterial cell, including surface charge. 73
S. epidermidis strains were examined for the presence of
ica operon, for the ability to form biofilm by Christensen test tube method and for the production of slime by Congo red agar method. Isoelectric points (p
I) of these strains were determined by means of Capillary Isoelectric Focusing. The biofilm negative strains focused near p
I value 2.3, while the p
I values of the biofilm positive strains were near 2.6. Isoelectric point is a useful criterion for the differentiation between biofilm-positive and biofilm-negative
S. epidermidis strains. |
| doi_str_mv | 10.1016/j.mimet.2006.10.008 |
| format | Article |
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S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The presence of this substance on the surface can affect physico-chemical properties of the bacterial cell, including surface charge. 73
S. epidermidis strains were examined for the presence of
ica operon, for the ability to form biofilm by Christensen test tube method and for the production of slime by Congo red agar method. Isoelectric points (p
I) of these strains were determined by means of Capillary Isoelectric Focusing. The biofilm negative strains focused near p
I value 2.3, while the p
I values of the biofilm positive strains were near 2.6. Isoelectric point is a useful criterion for the differentiation between biofilm-positive and biofilm-negative
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S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The presence of this substance on the surface can affect physico-chemical properties of the bacterial cell, including surface charge. 73
S. epidermidis strains were examined for the presence of
ica operon, for the ability to form biofilm by Christensen test tube method and for the production of slime by Congo red agar method. Isoelectric points (p
I) of these strains were determined by means of Capillary Isoelectric Focusing. The biofilm negative strains focused near p
I value 2.3, while the p
I values of the biofilm positive strains were near 2.6. Isoelectric point is a useful criterion for the differentiation between biofilm-positive and biofilm-negative
S. epidermidis strains.</description><subject>Amidohydrolases - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biofilm</subject><subject>Biofilm detection</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Capillary Isoelectric Focusing</subject><subject>Electrophoresis, Capillary - instrumentation</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Isoelectric Focusing - instrumentation</subject><subject>Isoelectric Focusing - methods</subject><subject>Isoelectric Point</subject><subject>Microbiology</subject><subject>Operon</subject><subject>Polysaccharides, Bacterial - metabolism</subject><subject>Slime</subject><subject>Staphylococcus epidermidis</subject><subject>Staphylococcus epidermidis - genetics</subject><subject>Staphylococcus epidermidis - growth & development</subject><subject>Staphylococcus epidermidis - isolation & purification</subject><subject>Ultrasonics</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi1ERZeWJ0BCvsAtix3HjnPggFa0VKrEoe3Zcuwx9cqJg51U6o2H4Al5kjrdlXqDiy3NfPPrn_kRek_JlhIqPu-3gx9g3taEiFLZEiJfoQ2VbV1JxrvXaFOotmoJrU_R25z3hFDOGvkGndKW8rZr6g3KOz35EHR6xFc5QgAzJ2_wRTRL9uNP_Pf3H3yXwS0BzzEG7GLCFuaC-Tji6PB8D7j30fkwrM1BPzf8iG9mPd0_hmiiKVoYJm8hDd76fI5OnA4Z3h3_M3R38e129726_nF5tft6XZmGyrnqrS02mXS9dkyT8tac9C23ljFRC25rKZnoTe-EaKTTDMAWxPKGu1qKhp2hTwfdKcVfC-RZDT4bKNuOEJesREdq0XLxX5B2XeEoLSA7gCbFnBM4NSU_lOMpStQaitqr51DUGspaLKGUqQ9H-aUfwL7MHFMowMcjoLPRwSU9Gp9fOMlbRsTq88uBg3K1Bw9JZeNhNGB9KokoG_0_jTwBoveuuQ</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Ruzicka, Filip</creator><creator>Horka, Marie</creator><creator>Hola, Veronika</creator><creator>Votava, Miroslav</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>Capillary Isoelectric Focusing — Useful tool for detection of the biofilm formation in Staphylococcus epidermidis</title><author>Ruzicka, Filip ; Horka, Marie ; Hola, Veronika ; Votava, Miroslav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-bdd71538fbaf3a0baf250b75dd336265d28836bcbf6648fa3eedf25d545f28643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amidohydrolases - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biofilm</topic><topic>Biofilm detection</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Capillary Isoelectric Focusing</topic><topic>Electrophoresis, Capillary - instrumentation</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Isoelectric Focusing - instrumentation</topic><topic>Isoelectric Focusing - methods</topic><topic>Isoelectric Point</topic><topic>Microbiology</topic><topic>Operon</topic><topic>Polysaccharides, Bacterial - metabolism</topic><topic>Slime</topic><topic>Staphylococcus epidermidis</topic><topic>Staphylococcus epidermidis - genetics</topic><topic>Staphylococcus epidermidis - growth & development</topic><topic>Staphylococcus epidermidis - isolation & purification</topic><topic>Ultrasonics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruzicka, Filip</creatorcontrib><creatorcontrib>Horka, Marie</creatorcontrib><creatorcontrib>Hola, Veronika</creatorcontrib><creatorcontrib>Votava, Miroslav</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruzicka, Filip</au><au>Horka, Marie</au><au>Hola, Veronika</au><au>Votava, Miroslav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary Isoelectric Focusing — Useful tool for detection of the biofilm formation in Staphylococcus epidermidis</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>68</volume><issue>3</issue><spage>530</spage><epage>535</epage><pages>530-535</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>The biofilm formation is an important factor of
S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The presence of this substance on the surface can affect physico-chemical properties of the bacterial cell, including surface charge. 73
S. epidermidis strains were examined for the presence of
ica operon, for the ability to form biofilm by Christensen test tube method and for the production of slime by Congo red agar method. Isoelectric points (p
I) of these strains were determined by means of Capillary Isoelectric Focusing. The biofilm negative strains focused near p
I value 2.3, while the p
I values of the biofilm positive strains were near 2.6. Isoelectric point is a useful criterion for the differentiation between biofilm-positive and biofilm-negative
S. epidermidis strains.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>17157942</pmid><doi>10.1016/j.mimet.2006.10.008</doi><tpages>6</tpages></addata></record> |
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| subjects | Amidohydrolases - genetics Bacterial Proteins - genetics Bacteriological methods and techniques used in bacteriology Bacteriology Biofilm Biofilm detection Biofilms - growth & development Biological and medical sciences Capillary Isoelectric Focusing Electrophoresis, Capillary - instrumentation Electrophoresis, Capillary - methods Fundamental and applied biological sciences. Psychology Humans Isoelectric Focusing - instrumentation Isoelectric Focusing - methods Isoelectric Point Microbiology Operon Polysaccharides, Bacterial - metabolism Slime Staphylococcus epidermidis Staphylococcus epidermidis - genetics Staphylococcus epidermidis - growth & development Staphylococcus epidermidis - isolation & purification Ultrasonics |
| title | Capillary Isoelectric Focusing — Useful tool for detection of the biofilm formation in Staphylococcus epidermidis |
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