Nonribosomal Synthesis of Fengycin on an Enzyme Complex Formed by Fengycin Synthetases
Fengycin, a lipopeptidic antibiotic, is synthesized nonribosomally by five fengycin synthetases (FenC, FenD, FenE, FenA, and FenB) in Bacillus subtilis F29-3. This work demonstrates that these fengycin synthetases interlock to form a chain, which coils into a 14.5-nm structure. In this chain, fengyc...
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| Veröffentlicht in: | The Journal of biological chemistry 2007-02, Vol.282 (8), p.5608-5616 |
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| container_title | The Journal of biological chemistry |
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| creator | Wu, Cheng-Yeu Chen, Chyi-Liang Lee, Yu-Hsiu Cheng, Yu-Chieh Wu, Ying-Chung Shu, Hung-Yu Goötz, Friedrich Liu, Shih-Tung |
| description | Fengycin, a lipopeptidic antibiotic, is synthesized nonribosomally by five fengycin synthetases (FenC, FenD, FenE, FenA, and FenB) in Bacillus subtilis F29-3. This work demonstrates that these fengycin synthetases interlock to form a chain, which coils into a 14.5-nm structure. In this chain, fengycin synthetases are linked in the order FenC-FenD-FenE-FenA-FenB by interactions between the C-terminal region of an upstream enzyme and the N-terminal region of its downstream partner enzyme, with their amino acid activation modules arranged colinearly with the amino acids in fengycin. This work also reveals that fengycin is synthesized on this fengycin synthetase chain, explaining how fengycin is synthesized efficiently and accurately. The results from this investigation demonstrate that forming a peptide synthetase complex is crucial to nonribosomal peptide synthesis. |
| doi_str_mv | 10.1074/jbc.M609726200 |
| format | Article |
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This work demonstrates that these fengycin synthetases interlock to form a chain, which coils into a 14.5-nm structure. In this chain, fengycin synthetases are linked in the order FenC-FenD-FenE-FenA-FenB by interactions between the C-terminal region of an upstream enzyme and the N-terminal region of its downstream partner enzyme, with their amino acid activation modules arranged colinearly with the amino acids in fengycin. This work also reveals that fengycin is synthesized on this fengycin synthetase chain, explaining how fengycin is synthesized efficiently and accurately. The results from this investigation demonstrate that forming a peptide synthetase complex is crucial to nonribosomal peptide synthesis.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M609726200</identifier><identifier>PMID: 17182617</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bacillus subtilis ; Bacillus subtilis - enzymology ; Bacillus subtilis - genetics ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Lipopeptides ; Lipoproteins - biosynthesis ; Multienzyme Complexes - genetics ; Multienzyme Complexes - metabolism ; Peptide Biosynthesis, Nucleic Acid-Independent - physiology ; Peptide Synthases - genetics ; Peptide Synthases - metabolism</subject><ispartof>The Journal of biological chemistry, 2007-02, Vol.282 (8), p.5608-5616</ispartof><rights>2007 © 2007 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-69447d1af1ed191451c8d110b6f8c5843f969c4b009839c4215f4a707d5a18503</citedby><cites>FETCH-LOGICAL-c530t-69447d1af1ed191451c8d110b6f8c5843f969c4b009839c4215f4a707d5a18503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17182617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Cheng-Yeu</creatorcontrib><creatorcontrib>Chen, Chyi-Liang</creatorcontrib><creatorcontrib>Lee, Yu-Hsiu</creatorcontrib><creatorcontrib>Cheng, Yu-Chieh</creatorcontrib><creatorcontrib>Wu, Ying-Chung</creatorcontrib><creatorcontrib>Shu, Hung-Yu</creatorcontrib><creatorcontrib>Goötz, Friedrich</creatorcontrib><creatorcontrib>Liu, Shih-Tung</creatorcontrib><title>Nonribosomal Synthesis of Fengycin on an Enzyme Complex Formed by Fengycin Synthetases</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Fengycin, a lipopeptidic antibiotic, is synthesized nonribosomally by five fengycin synthetases (FenC, FenD, FenE, FenA, and FenB) in Bacillus subtilis F29-3. 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The results from this investigation demonstrate that forming a peptide synthetase complex is crucial to nonribosomal peptide synthesis.</description><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - enzymology</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Lipopeptides</subject><subject>Lipoproteins - biosynthesis</subject><subject>Multienzyme Complexes - genetics</subject><subject>Multienzyme Complexes - metabolism</subject><subject>Peptide Biosynthesis, Nucleic Acid-Independent - physiology</subject><subject>Peptide Synthases - genetics</subject><subject>Peptide Synthases - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGP0zAQRi0EYkvhyhEsDtxSZhw7sY-o2gLSAodlETfLcZzWqyQudgqEX49RKvWEmIvn8Oab0TMhzxE2CDV_c9_YzccKVM0qBvCArBBkWZQCvz0kKwCGhWJCXpEnKd1DLq7wMbnCGiWrsF6Rr5_CGH0TUhhMT2_ncTq45BMNHd25cT9bP9IwUjPS6_H3PDi6DcOxd7_oLsTBtbSZL9wyPZnk0lPyqDN9cs_O75rc7a6_bN8XN5_ffdi-vSmsKGEqKsV53aLp0LWokAu0skWEpuqkFZKXnaqU5Q2AkmVuGIqOmxrqVhiUAso1eb3kHmP4fnJp0oNP1vW9GV04JV0pYFyi-i-IStRM5o1rsllAG0NK0XX6GP1g4qwR9F_lOivXF-V54MU5-dRkIxf87DgDrxbg4PeHnz463fhgD27QTDIttajyl63JywXqTNBmH33Sd7cMsIS8suYVy4RcCJd9_vAu6mS9G61rc6SddBv8v078A1DRorc</recordid><startdate>20070223</startdate><enddate>20070223</enddate><creator>Wu, Cheng-Yeu</creator><creator>Chen, Chyi-Liang</creator><creator>Lee, Yu-Hsiu</creator><creator>Cheng, Yu-Chieh</creator><creator>Wu, Ying-Chung</creator><creator>Shu, Hung-Yu</creator><creator>Goötz, Friedrich</creator><creator>Liu, Shih-Tung</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20070223</creationdate><title>Nonribosomal Synthesis of Fengycin on an Enzyme Complex Formed by Fengycin Synthetases</title><author>Wu, Cheng-Yeu ; Chen, Chyi-Liang ; Lee, Yu-Hsiu ; Cheng, Yu-Chieh ; Wu, Ying-Chung ; Shu, Hung-Yu ; Goötz, Friedrich ; Liu, Shih-Tung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-69447d1af1ed191451c8d110b6f8c5843f969c4b009839c4215f4a707d5a18503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - enzymology</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Lipopeptides</topic><topic>Lipoproteins - biosynthesis</topic><topic>Multienzyme Complexes - genetics</topic><topic>Multienzyme Complexes - metabolism</topic><topic>Peptide Biosynthesis, Nucleic Acid-Independent - physiology</topic><topic>Peptide Synthases - genetics</topic><topic>Peptide Synthases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Cheng-Yeu</creatorcontrib><creatorcontrib>Chen, Chyi-Liang</creatorcontrib><creatorcontrib>Lee, Yu-Hsiu</creatorcontrib><creatorcontrib>Cheng, Yu-Chieh</creatorcontrib><creatorcontrib>Wu, Ying-Chung</creatorcontrib><creatorcontrib>Shu, Hung-Yu</creatorcontrib><creatorcontrib>Goötz, Friedrich</creatorcontrib><creatorcontrib>Liu, Shih-Tung</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Cheng-Yeu</au><au>Chen, Chyi-Liang</au><au>Lee, Yu-Hsiu</au><au>Cheng, Yu-Chieh</au><au>Wu, Ying-Chung</au><au>Shu, Hung-Yu</au><au>Goötz, Friedrich</au><au>Liu, Shih-Tung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nonribosomal Synthesis of Fengycin on an Enzyme Complex Formed by Fengycin Synthetases</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2007-02-23</date><risdate>2007</risdate><volume>282</volume><issue>8</issue><spage>5608</spage><epage>5616</epage><pages>5608-5616</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Fengycin, a lipopeptidic antibiotic, is synthesized nonribosomally by five fengycin synthetases (FenC, FenD, FenE, FenA, and FenB) in Bacillus subtilis F29-3. This work demonstrates that these fengycin synthetases interlock to form a chain, which coils into a 14.5-nm structure. In this chain, fengycin synthetases are linked in the order FenC-FenD-FenE-FenA-FenB by interactions between the C-terminal region of an upstream enzyme and the N-terminal region of its downstream partner enzyme, with their amino acid activation modules arranged colinearly with the amino acids in fengycin. This work also reveals that fengycin is synthesized on this fengycin synthetase chain, explaining how fengycin is synthesized efficiently and accurately. The results from this investigation demonstrate that forming a peptide synthetase complex is crucial to nonribosomal peptide synthesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17182617</pmid><doi>10.1074/jbc.M609726200</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacterial Proteins - genetics Bacterial Proteins - metabolism Lipopeptides Lipoproteins - biosynthesis Multienzyme Complexes - genetics Multienzyme Complexes - metabolism Peptide Biosynthesis, Nucleic Acid-Independent - physiology Peptide Synthases - genetics Peptide Synthases - metabolism |
| title | Nonribosomal Synthesis of Fengycin on an Enzyme Complex Formed by Fengycin Synthetases |
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