Development and evaluation of a diagnostic PCR for Mycoplasma synoviae using primers located in the intergenic spacer region and the 23S rRNA gene
Mycoplasma synoviae (Ms) is an important pathogen of poultry, causing economic losses to this industry. Early and reliable diagnosis is a key to controlling the spread of this organism. In this study, a polymerase chain reaction with one primer based on the intergenic spacer region (ISR) was validat...
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| Veröffentlicht in: | Veterinary microbiology 2006-11, Vol.118 (1), p.76-82 |
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| creator | Ramírez, Ana S. Naylor, Clive J. Hammond, Philip P. Bradbury, Janet M. |
| description | Mycoplasma synoviae (Ms) is an important pathogen of poultry, causing economic losses to this industry. Early and reliable diagnosis is a key to controlling the spread of this organism. In this study, a polymerase chain reaction with one primer based on the intergenic spacer region (ISR) was validated for detection of Ms. The ISR primer was paired with a general primer from within the 23S rRNA gene. The PCR primers were tested with the 22 other recognised avian
Mycoplasma species to check the specificity and with 21 field isolates of Ms from various hosts and countries, and with several swab samples. The PCR appeared to be specific and sensitive. Four different sample preparation methods were compared for use in this PCR, and the amplification protocol was compared with three others, confirming the comparative sensitivity of the new PCR. |
| doi_str_mv | 10.1016/j.vetmic.2006.06.021 |
| format | Article |
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Mycoplasma species to check the specificity and with 21 field isolates of Ms from various hosts and countries, and with several swab samples. The PCR appeared to be specific and sensitive. Four different sample preparation methods were compared for use in this PCR, and the amplification protocol was compared with three others, confirming the comparative sensitivity of the new PCR.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2006.06.021</identifier><identifier>PMID: 16899346</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>animal pathogenic bacteria ; Animals ; Bacteriology ; Biological and medical sciences ; Cells, Cultured ; Chickens ; Diagnosis ; diagnostic techniques ; disease control ; disease diagnosis ; DNA Primers ; DNA, Intergenic ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; intergenic transcribed spacers ; joint diseases ; microbial genetics ; Microbiology ; Miscellaneous ; molecular sequence data ; Mollicutes ; Mycoplasma ; Mycoplasma Infections - diagnosis ; Mycoplasma Infections - veterinary ; Mycoplasma synoviae ; Mycoplasma synoviae - isolation & purification ; mycoplasmosis ; pathogen identification ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; poultry ; poultry diseases ; Poultry Diseases - diagnosis ; pretreatment ; respiratory tract diseases ; ribosomal RNA ; RNA, Ribosomal, 23S - genetics ; Sensitivity and Specificity ; sigma factors ; Species Specificity ; test sensitivity ; test specificity ; Turkeys</subject><ispartof>Veterinary microbiology, 2006-11, Vol.118 (1), p.76-82</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-60f8f08e632ace020c09bd5d0ab0abd58a2d00aac31a351f88802a9338c5d99d3</citedby><cites>FETCH-LOGICAL-c445t-60f8f08e632ace020c09bd5d0ab0abd58a2d00aac31a351f88802a9338c5d99d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2006.06.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18255957$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16899346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramírez, Ana S.</creatorcontrib><creatorcontrib>Naylor, Clive J.</creatorcontrib><creatorcontrib>Hammond, Philip P.</creatorcontrib><creatorcontrib>Bradbury, Janet M.</creatorcontrib><title>Development and evaluation of a diagnostic PCR for Mycoplasma synoviae using primers located in the intergenic spacer region and the 23S rRNA gene</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Mycoplasma synoviae (Ms) is an important pathogen of poultry, causing economic losses to this industry. Early and reliable diagnosis is a key to controlling the spread of this organism. In this study, a polymerase chain reaction with one primer based on the intergenic spacer region (ISR) was validated for detection of Ms. The ISR primer was paired with a general primer from within the 23S rRNA gene. The PCR primers were tested with the 22 other recognised avian
Mycoplasma species to check the specificity and with 21 field isolates of Ms from various hosts and countries, and with several swab samples. The PCR appeared to be specific and sensitive. Four different sample preparation methods were compared for use in this PCR, and the amplification protocol was compared with three others, confirming the comparative sensitivity of the new PCR.</description><subject>animal pathogenic bacteria</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chickens</subject><subject>Diagnosis</subject><subject>diagnostic techniques</subject><subject>disease control</subject><subject>disease diagnosis</subject><subject>DNA Primers</subject><subject>DNA, Intergenic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>intergenic transcribed spacers</subject><subject>joint diseases</subject><subject>microbial genetics</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>molecular sequence data</subject><subject>Mollicutes</subject><subject>Mycoplasma</subject><subject>Mycoplasma Infections - diagnosis</subject><subject>Mycoplasma Infections - veterinary</subject><subject>Mycoplasma synoviae</subject><subject>Mycoplasma synoviae - isolation & purification</subject><subject>mycoplasmosis</subject><subject>pathogen identification</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>poultry</subject><subject>poultry diseases</subject><subject>Poultry Diseases - diagnosis</subject><subject>pretreatment</subject><subject>respiratory tract diseases</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 23S - genetics</subject><subject>Sensitivity and Specificity</subject><subject>sigma factors</subject><subject>Species Specificity</subject><subject>test sensitivity</subject><subject>test specificity</subject><subject>Turkeys</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OEzEQhEcIxIaFN0DgC9wS2vZ4Yl-QVuFXWn60y56tjt0THM2Ms_YkUl6DJ8ajRNobSCX15evqVlVVveSw4MCbd9vFgcY-uIUAaBaTBH9UzbheyrlQtXhczUAu9ZxzqS6qZzlvAaA2DTytLnijjZF1M6v-fKADdXHX0zAyHDyjA3Z7HEMcWGwZMh9wM8Q8Bsd-rm5YGxP7dnRx12HukeXjEA8Bie1zGDZsl0JPKbMuOhzJszCw8TeVMVLa0FA88g4dJZZoM12YDk6AkLcs3Xy_YgWi59WTFrtML87zsrr79PHX6sv8-sfnr6ur67mrazXOG2h1C5oaKYolCHBg1l55wHWRVxqFB0B0kqNUvNVag0AjpXbKG-PlZfX25LtL8X5PebR9yI66DgeK-2wbUxKtTf1fkBupGwm6gPUJdCnmnKi1UyCYjpaDnUqzW3sqzU6l2UmCl7VXZ__9uif_sHRuqQBvzgBmh12bcHAhP3BaKGXUsnCvT1yL0eImFebuVgCXwDk3SkxO708ElWAPgZLNLtDgyIdEbrQ-hn__-hcLesGx</recordid><startdate>20061126</startdate><enddate>20061126</enddate><creator>Ramírez, Ana S.</creator><creator>Naylor, Clive J.</creator><creator>Hammond, Philip P.</creator><creator>Bradbury, Janet M.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061126</creationdate><title>Development and evaluation of a diagnostic PCR for Mycoplasma synoviae using primers located in the intergenic spacer region and the 23S rRNA gene</title><author>Ramírez, Ana S. ; Naylor, Clive J. ; Hammond, Philip P. ; Bradbury, Janet M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-60f8f08e632ace020c09bd5d0ab0abd58a2d00aac31a351f88802a9338c5d99d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>animal pathogenic bacteria</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chickens</topic><topic>Diagnosis</topic><topic>diagnostic techniques</topic><topic>disease control</topic><topic>disease diagnosis</topic><topic>DNA Primers</topic><topic>DNA, Intergenic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>intergenic transcribed spacers</topic><topic>joint diseases</topic><topic>microbial genetics</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>molecular sequence data</topic><topic>Mollicutes</topic><topic>Mycoplasma</topic><topic>Mycoplasma Infections - diagnosis</topic><topic>Mycoplasma Infections - veterinary</topic><topic>Mycoplasma synoviae</topic><topic>Mycoplasma synoviae - isolation & purification</topic><topic>mycoplasmosis</topic><topic>pathogen identification</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>poultry</topic><topic>poultry diseases</topic><topic>Poultry Diseases - diagnosis</topic><topic>pretreatment</topic><topic>respiratory tract diseases</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal, 23S - genetics</topic><topic>Sensitivity and Specificity</topic><topic>sigma factors</topic><topic>Species Specificity</topic><topic>test sensitivity</topic><topic>test specificity</topic><topic>Turkeys</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramírez, Ana S.</creatorcontrib><creatorcontrib>Naylor, Clive J.</creatorcontrib><creatorcontrib>Hammond, Philip P.</creatorcontrib><creatorcontrib>Bradbury, Janet M.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramírez, Ana S.</au><au>Naylor, Clive J.</au><au>Hammond, Philip P.</au><au>Bradbury, Janet M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and evaluation of a diagnostic PCR for Mycoplasma synoviae using primers located in the intergenic spacer region and the 23S rRNA gene</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2006-11-26</date><risdate>2006</risdate><volume>118</volume><issue>1</issue><spage>76</spage><epage>82</epage><pages>76-82</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Mycoplasma synoviae (Ms) is an important pathogen of poultry, causing economic losses to this industry. Early and reliable diagnosis is a key to controlling the spread of this organism. In this study, a polymerase chain reaction with one primer based on the intergenic spacer region (ISR) was validated for detection of Ms. The ISR primer was paired with a general primer from within the 23S rRNA gene. The PCR primers were tested with the 22 other recognised avian
Mycoplasma species to check the specificity and with 21 field isolates of Ms from various hosts and countries, and with several swab samples. The PCR appeared to be specific and sensitive. Four different sample preparation methods were compared for use in this PCR, and the amplification protocol was compared with three others, confirming the comparative sensitivity of the new PCR.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16899346</pmid><doi>10.1016/j.vetmic.2006.06.021</doi><tpages>7</tpages></addata></record> |
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| subjects | animal pathogenic bacteria Animals Bacteriology Biological and medical sciences Cells, Cultured Chickens Diagnosis diagnostic techniques disease control disease diagnosis DNA Primers DNA, Intergenic Fundamental and applied biological sciences. Psychology Gene Amplification intergenic transcribed spacers joint diseases microbial genetics Microbiology Miscellaneous molecular sequence data Mollicutes Mycoplasma Mycoplasma Infections - diagnosis Mycoplasma Infections - veterinary Mycoplasma synoviae Mycoplasma synoviae - isolation & purification mycoplasmosis pathogen identification Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary poultry poultry diseases Poultry Diseases - diagnosis pretreatment respiratory tract diseases ribosomal RNA RNA, Ribosomal, 23S - genetics Sensitivity and Specificity sigma factors Species Specificity test sensitivity test specificity Turkeys |
| title | Development and evaluation of a diagnostic PCR for Mycoplasma synoviae using primers located in the intergenic spacer region and the 23S rRNA gene |
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