Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes
Erp1 (also called Emi2), an inhibitor of the APC/C ubiquitin ligase, is a key component of cytostatic factor (CSF) responsible for Meta-II arrest in vertebrate eggs. Reportedly, however, Erp1 is expressed even during meiosis I in Xenopus oocytes. If so, it is a puzzle why normally maturing oocytes c...
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| Veröffentlicht in: | Developmental biology 2007-03, Vol.303 (1), p.157-164 |
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| creator | Ohe, Munemichi Inoue, Daigo Kanemori, Yoshinori Sagata, Noriyuki |
| description | Erp1 (also called Emi2), an inhibitor of the APC/C ubiquitin ligase, is a key component of cytostatic factor (CSF) responsible for Meta-II arrest in vertebrate eggs. Reportedly, however, Erp1 is expressed even during meiosis I in
Xenopus oocytes. If so, it is a puzzle why normally maturing oocytes cannot arrest at Meta-I. Here, we show that actually Erp1 synthesis begins only around the end of meiosis I in
Xenopus oocytes, and that specific inhibition of Erp1 synthesis by morpholino oligos prevents entry into meiosis II. Furthermore, we demonstrate that premature, ectopic expression of Erp1 at physiological Meta-II levels can arrest maturing oocytes at Meta-I. Thus, our results show the essential role for Erp1 in the meiosis I/meiosis II transition in
Xenopus oocytes and can explain why normally maturing oocytes cannot arrest at Meta-I. |
| doi_str_mv | 10.1016/j.ydbio.2006.10.044 |
| format | Article |
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Xenopus oocytes. If so, it is a puzzle why normally maturing oocytes cannot arrest at Meta-I. Here, we show that actually Erp1 synthesis begins only around the end of meiosis I in
Xenopus oocytes, and that specific inhibition of Erp1 synthesis by morpholino oligos prevents entry into meiosis II. Furthermore, we demonstrate that premature, ectopic expression of Erp1 at physiological Meta-II levels can arrest maturing oocytes at Meta-I. Thus, our results show the essential role for Erp1 in the meiosis I/meiosis II transition in
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Xenopus oocytes. If so, it is a puzzle why normally maturing oocytes cannot arrest at Meta-I. Here, we show that actually Erp1 synthesis begins only around the end of meiosis I in
Xenopus oocytes, and that specific inhibition of Erp1 synthesis by morpholino oligos prevents entry into meiosis II. Furthermore, we demonstrate that premature, ectopic expression of Erp1 at physiological Meta-II levels can arrest maturing oocytes at Meta-I. Thus, our results show the essential role for Erp1 in the meiosis I/meiosis II transition in
Xenopus oocytes and can explain why normally maturing oocytes cannot arrest at Meta-I.</description><subject>Animals</subject><subject>APC/C</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Erp1/Emi2</subject><subject>F-Box Proteins - metabolism</subject><subject>Freshwater</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Meiosis</subject><subject>Meiosis - physiology</subject><subject>Oligonucleotides</subject><subject>Oocyte</subject><subject>Oocytes - physiology</subject><subject>Protein Kinases - metabolism</subject><subject>Xenopus</subject><subject>Xenopus - physiology</subject><subject>Xenopus Proteins - metabolism</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMoWj9-gSA5edt28rHZzcGDlKqFgpcK3sJuMosp3U1NtkL_vVtb8Kan4Z15ZgYeQm4ZjBkwNVmNd672YcwB1NAZg5QnZMRA51mu5PspGQEwnjEF6oJcprQCAFGW4pxcsIJJxqEckeUsbthk1npOfaKYEna9r9a0CZH2H0hb9CENkzntw28YUqy65HsfOuo7-o5d2GwTDcHuekzX5Kyp1glvjvWKvD3NltOXbPH6PJ8-LjIrte6z2uWykIXAkmNd88q6EnVRSl44a60oc5Zbp50GBZhzxWzjXKVQYSNAac7EFbk_3N3E8LnF1JvWJ4vrddVh2CajNDBdFOW_IAchQKp8AMUBtDGkFLExm-jbKu4MA7O3blbmx7rZW983B-vD1t3x_LZu0f3uHDUPwMMBwMHGl8dokvXYWXQ-ou2NC_7PB9_TdpPN</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Ohe, Munemichi</creator><creator>Inoue, Daigo</creator><creator>Kanemori, Yoshinori</creator><creator>Sagata, Noriyuki</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes</title><author>Ohe, Munemichi ; Inoue, Daigo ; Kanemori, Yoshinori ; Sagata, Noriyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-bd547473e82ebb2acd8e978427dccc38515cd9d9060e5261cfdda6e6ef3069213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>APC/C</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Erp1/Emi2</topic><topic>F-Box Proteins - metabolism</topic><topic>Freshwater</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Meiosis</topic><topic>Meiosis - physiology</topic><topic>Oligonucleotides</topic><topic>Oocyte</topic><topic>Oocytes - physiology</topic><topic>Protein Kinases - metabolism</topic><topic>Xenopus</topic><topic>Xenopus - physiology</topic><topic>Xenopus Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohe, Munemichi</creatorcontrib><creatorcontrib>Inoue, Daigo</creatorcontrib><creatorcontrib>Kanemori, Yoshinori</creatorcontrib><creatorcontrib>Sagata, Noriyuki</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohe, Munemichi</au><au>Inoue, Daigo</au><au>Kanemori, Yoshinori</au><au>Sagata, Noriyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>303</volume><issue>1</issue><spage>157</spage><epage>164</epage><pages>157-164</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Erp1 (also called Emi2), an inhibitor of the APC/C ubiquitin ligase, is a key component of cytostatic factor (CSF) responsible for Meta-II arrest in vertebrate eggs. Reportedly, however, Erp1 is expressed even during meiosis I in
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Xenopus oocytes, and that specific inhibition of Erp1 synthesis by morpholino oligos prevents entry into meiosis II. Furthermore, we demonstrate that premature, ectopic expression of Erp1 at physiological Meta-II levels can arrest maturing oocytes at Meta-I. Thus, our results show the essential role for Erp1 in the meiosis I/meiosis II transition in
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| ispartof | Developmental biology, 2007-03, Vol.303 (1), p.157-164 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals APC/C Cell Cycle Proteins - metabolism Erp1/Emi2 F-Box Proteins - metabolism Freshwater Gene Expression Regulation, Developmental Immunoblotting Immunohistochemistry Meiosis Meiosis - physiology Oligonucleotides Oocyte Oocytes - physiology Protein Kinases - metabolism Xenopus Xenopus - physiology Xenopus Proteins - metabolism |
| title | Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes |
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