Micellar electrokinetic chromatography–electrospray ionization mass spectrometry for the identification of drug impurities

Previously, we have presented a system hyphenating continuous micellar electrokinetic chromatography (MEKC) with electrospray ionization mass spectrometry (ESI-MS). Here we evaluate this technique for its applicability in impurity profiling of drugs using galantamine and ipratropium as test samples....

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-11, Vol.843 (2), p.283-288
Hauptverfasser: Mol, Roelof, Kragt, Else, Jimidar, Ilias, de Jong, Gerhardus J., Somsen, Govert W.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 288
container_issue 2
container_start_page 283
container_title Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
container_volume 843
creator Mol, Roelof
Kragt, Else
Jimidar, Ilias
de Jong, Gerhardus J.
Somsen, Govert W.
description Previously, we have presented a system hyphenating continuous micellar electrokinetic chromatography (MEKC) with electrospray ionization mass spectrometry (ESI-MS). Here we evaluate this technique for its applicability in impurity profiling of drugs using galantamine and ipratropium as test samples. A background electrolyte (BGE) of 10 mM sodium phosphate (pH 7.5), 12.5–15% acetonitrile and 20 mM sodium dodecylsulfate (SDS) was used for the MEKC–MS analysis of a galantamine sample containing a number of related impurities, and a heat-treated solution of ipratropium containing a number of unknown degradation products. MEKC provided efficient separation of all sample constituents. Despite the presence of non-volatile BGEs, all impurities in the galantamine sample could be detected by ESI-MS in their respective extracted ion traces (XICs) with a detection sensitivity in the sub-μg/ml range (full-scan mode). MS/MS detection provided useful product spectra allowing the structural characterization of the respective galantamine impurities. With the MEKC–MS/MS system, two degradation products could be revealed and identified in the heat-stressed ipratropium sample. The presented method shows good potential for the detection and structure elucidation of minor impurities in drug substances.
doi_str_mv 10.1016/j.jchromb.2006.06.019
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69018132</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S157002320600506X</els_id><sourcerecordid>69018132</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-845f9ea01a2faaf454fdddb5e5d81769344659a98834b2b4b7b9b4cda681f8603</originalsourceid><addsrcrecordid>eNqFkM2KFDEQgIO4uOvqIyi56K1n89ed9Elk8Q9W9qLgLaSTyk7G_jNJCyMe9h18Q5_EzEzDHoWCCtRXVakPoReUbCihzdVus7PbOA3dhhHSbA5B20fogirJKy6bb4_Lu5akIoyzc_Q0pR0hVBLJn6Bz2iguWS0u0O_PwULfm4ihB5vj9D2MkIPFx-EmT3fRzNv93_s_az3N0exxmMbwy-SS8GBSwmk-FgfIcY_9FHHeAg4Oxhx8sCdw8tjF5Q6HYV5iyAHSM3TmTZ_g-Zov0df3775cf6xubj98un57U1nBWK6UqH0LhlDDvDFe1MI757oaaqeobFouRFO3plWKi451opNd2wnrTKOoVw3hl-j1ae4cpx8LpKyHkI5njzAtSTctoYpyVsD6BNpyaIrg9RzDYOJeU6IP2vVOr9r1Qbs-BG1L38t1wdIN4B66Vs8FeLUCJlnT-2hGG9IDpxhvlVSFe3PioOj4GSDqZAOMFlyIxbB2U_jPV_4BmXSpBg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69018132</pqid></control><display><type>article</type><title>Micellar electrokinetic chromatography–electrospray ionization mass spectrometry for the identification of drug impurities</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Mol, Roelof ; Kragt, Else ; Jimidar, Ilias ; de Jong, Gerhardus J. ; Somsen, Govert W.</creator><creatorcontrib>Mol, Roelof ; Kragt, Else ; Jimidar, Ilias ; de Jong, Gerhardus J. ; Somsen, Govert W.</creatorcontrib><description>Previously, we have presented a system hyphenating continuous micellar electrokinetic chromatography (MEKC) with electrospray ionization mass spectrometry (ESI-MS). Here we evaluate this technique for its applicability in impurity profiling of drugs using galantamine and ipratropium as test samples. A background electrolyte (BGE) of 10 mM sodium phosphate (pH 7.5), 12.5–15% acetonitrile and 20 mM sodium dodecylsulfate (SDS) was used for the MEKC–MS analysis of a galantamine sample containing a number of related impurities, and a heat-treated solution of ipratropium containing a number of unknown degradation products. MEKC provided efficient separation of all sample constituents. Despite the presence of non-volatile BGEs, all impurities in the galantamine sample could be detected by ESI-MS in their respective extracted ion traces (XICs) with a detection sensitivity in the sub-μg/ml range (full-scan mode). MS/MS detection provided useful product spectra allowing the structural characterization of the respective galantamine impurities. With the MEKC–MS/MS system, two degradation products could be revealed and identified in the heat-stressed ipratropium sample. The presented method shows good potential for the detection and structure elucidation of minor impurities in drug substances.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2006.06.019</identifier><identifier>PMID: 16837254</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, Micellar Electrokinetic Capillary - methods ; Drug Contamination ; Fundamental and applied biological sciences. Psychology ; Galantamine - analysis ; General pharmacology ; Impurity profiling ; Ipratropium - analysis ; Mass spectrometry (MS) ; Medical sciences ; Micellar electrokinetic chromatography (MEKC) ; Pharmacology. Drug treatments ; Spectrometry, Mass, Electrospray Ionization - methods ; Tandem Mass Spectrometry ; Ultraviolet Rays</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2006-11, Vol.843 (2), p.283-288</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-845f9ea01a2faaf454fdddb5e5d81769344659a98834b2b4b7b9b4cda681f8603</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2006.06.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=18239878$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16837254$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mol, Roelof</creatorcontrib><creatorcontrib>Kragt, Else</creatorcontrib><creatorcontrib>Jimidar, Ilias</creatorcontrib><creatorcontrib>de Jong, Gerhardus J.</creatorcontrib><creatorcontrib>Somsen, Govert W.</creatorcontrib><title>Micellar electrokinetic chromatography–electrospray ionization mass spectrometry for the identification of drug impurities</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>Previously, we have presented a system hyphenating continuous micellar electrokinetic chromatography (MEKC) with electrospray ionization mass spectrometry (ESI-MS). Here we evaluate this technique for its applicability in impurity profiling of drugs using galantamine and ipratropium as test samples. A background electrolyte (BGE) of 10 mM sodium phosphate (pH 7.5), 12.5–15% acetonitrile and 20 mM sodium dodecylsulfate (SDS) was used for the MEKC–MS analysis of a galantamine sample containing a number of related impurities, and a heat-treated solution of ipratropium containing a number of unknown degradation products. MEKC provided efficient separation of all sample constituents. Despite the presence of non-volatile BGEs, all impurities in the galantamine sample could be detected by ESI-MS in their respective extracted ion traces (XICs) with a detection sensitivity in the sub-μg/ml range (full-scan mode). MS/MS detection provided useful product spectra allowing the structural characterization of the respective galantamine impurities. With the MEKC–MS/MS system, two degradation products could be revealed and identified in the heat-stressed ipratropium sample. The presented method shows good potential for the detection and structure elucidation of minor impurities in drug substances.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Micellar Electrokinetic Capillary - methods</subject><subject>Drug Contamination</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galantamine - analysis</subject><subject>General pharmacology</subject><subject>Impurity profiling</subject><subject>Ipratropium - analysis</subject><subject>Mass spectrometry (MS)</subject><subject>Medical sciences</subject><subject>Micellar electrokinetic chromatography (MEKC)</subject><subject>Pharmacology. Drug treatments</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Tandem Mass Spectrometry</subject><subject>Ultraviolet Rays</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM2KFDEQgIO4uOvqIyi56K1n89ed9Elk8Q9W9qLgLaSTyk7G_jNJCyMe9h18Q5_EzEzDHoWCCtRXVakPoReUbCihzdVus7PbOA3dhhHSbA5B20fogirJKy6bb4_Lu5akIoyzc_Q0pR0hVBLJn6Bz2iguWS0u0O_PwULfm4ihB5vj9D2MkIPFx-EmT3fRzNv93_s_az3N0exxmMbwy-SS8GBSwmk-FgfIcY_9FHHeAg4Oxhx8sCdw8tjF5Q6HYV5iyAHSM3TmTZ_g-Zov0df3775cf6xubj98un57U1nBWK6UqH0LhlDDvDFe1MI757oaaqeobFouRFO3plWKi451opNd2wnrTKOoVw3hl-j1ae4cpx8LpKyHkI5njzAtSTctoYpyVsD6BNpyaIrg9RzDYOJeU6IP2vVOr9r1Qbs-BG1L38t1wdIN4B66Vs8FeLUCJlnT-2hGG9IDpxhvlVSFe3PioOj4GSDqZAOMFlyIxbB2U_jPV_4BmXSpBg</recordid><startdate>20061107</startdate><enddate>20061107</enddate><creator>Mol, Roelof</creator><creator>Kragt, Else</creator><creator>Jimidar, Ilias</creator><creator>de Jong, Gerhardus J.</creator><creator>Somsen, Govert W.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20061107</creationdate><title>Micellar electrokinetic chromatography–electrospray ionization mass spectrometry for the identification of drug impurities</title><author>Mol, Roelof ; Kragt, Else ; Jimidar, Ilias ; de Jong, Gerhardus J. ; Somsen, Govert W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-845f9ea01a2faaf454fdddb5e5d81769344659a98834b2b4b7b9b4cda681f8603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Micellar Electrokinetic Capillary - methods</topic><topic>Drug Contamination</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galantamine - analysis</topic><topic>General pharmacology</topic><topic>Impurity profiling</topic><topic>Ipratropium - analysis</topic><topic>Mass spectrometry (MS)</topic><topic>Medical sciences</topic><topic>Micellar electrokinetic chromatography (MEKC)</topic><topic>Pharmacology. Drug treatments</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Tandem Mass Spectrometry</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mol, Roelof</creatorcontrib><creatorcontrib>Kragt, Else</creatorcontrib><creatorcontrib>Jimidar, Ilias</creatorcontrib><creatorcontrib>de Jong, Gerhardus J.</creatorcontrib><creatorcontrib>Somsen, Govert W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mol, Roelof</au><au>Kragt, Else</au><au>Jimidar, Ilias</au><au>de Jong, Gerhardus J.</au><au>Somsen, Govert W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Micellar electrokinetic chromatography–electrospray ionization mass spectrometry for the identification of drug impurities</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2006-11-07</date><risdate>2006</risdate><volume>843</volume><issue>2</issue><spage>283</spage><epage>288</epage><pages>283-288</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>Previously, we have presented a system hyphenating continuous micellar electrokinetic chromatography (MEKC) with electrospray ionization mass spectrometry (ESI-MS). Here we evaluate this technique for its applicability in impurity profiling of drugs using galantamine and ipratropium as test samples. A background electrolyte (BGE) of 10 mM sodium phosphate (pH 7.5), 12.5–15% acetonitrile and 20 mM sodium dodecylsulfate (SDS) was used for the MEKC–MS analysis of a galantamine sample containing a number of related impurities, and a heat-treated solution of ipratropium containing a number of unknown degradation products. MEKC provided efficient separation of all sample constituents. Despite the presence of non-volatile BGEs, all impurities in the galantamine sample could be detected by ESI-MS in their respective extracted ion traces (XICs) with a detection sensitivity in the sub-μg/ml range (full-scan mode). MS/MS detection provided useful product spectra allowing the structural characterization of the respective galantamine impurities. With the MEKC–MS/MS system, two degradation products could be revealed and identified in the heat-stressed ipratropium sample. The presented method shows good potential for the detection and structure elucidation of minor impurities in drug substances.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16837254</pmid><doi>10.1016/j.jchromb.2006.06.019</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1570-0232
ispartof Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2006-11, Vol.843 (2), p.283-288
issn 1570-0232
1873-376X
language eng
recordid cdi_proquest_miscellaneous_69018132
source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, Micellar Electrokinetic Capillary - methods
Drug Contamination
Fundamental and applied biological sciences. Psychology
Galantamine - analysis
General pharmacology
Impurity profiling
Ipratropium - analysis
Mass spectrometry (MS)
Medical sciences
Micellar electrokinetic chromatography (MEKC)
Pharmacology. Drug treatments
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry
Ultraviolet Rays
title Micellar electrokinetic chromatography–electrospray ionization mass spectrometry for the identification of drug impurities
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A15%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Micellar%20electrokinetic%20chromatography%E2%80%93electrospray%20ionization%20mass%20spectrometry%20for%20the%20identification%20of%20drug%20impurities&rft.jtitle=Journal%20of%20chromatography.%20B,%20Analytical%20technologies%20in%20the%20biomedical%20and%20life%20sciences&rft.au=Mol,%20Roelof&rft.date=2006-11-07&rft.volume=843&rft.issue=2&rft.spage=283&rft.epage=288&rft.pages=283-288&rft.issn=1570-0232&rft.eissn=1873-376X&rft_id=info:doi/10.1016/j.jchromb.2006.06.019&rft_dat=%3Cproquest_cross%3E69018132%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69018132&rft_id=info:pmid/16837254&rft_els_id=S157002320600506X&rfr_iscdi=true