Cloning, Expression, and Characterization of Bacillus sp. snu-7 Inulin Fructotransferase
A gene encoding inulin fructotransferase (di-D-fructofuranose 1,2':2,3' dianhydride [DFA Ⅲ]-producing IFTase, EC 4.2.2.18) from Bacillus sp. snu-7 was cloned. This gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a...
Gespeichert in:
| Veröffentlicht in: | Journal of microbiology and biotechnology 2007-01, Vol.17 (1), p.37-43 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 43 |
|---|---|
| container_issue | 1 |
| container_start_page | 37 |
| container_title | Journal of microbiology and biotechnology |
| container_volume | 17 |
| creator | Kim, C.S. (Seoul National University, Seoul, Republic of Korea) Hong, C.K. (Seoul National University, Seoul, Republic of Korea) Kim, K.Y. (Seoul National University, Seoul, Republic of Korea) Wang, Xiu Ling (Agricultural University of Hebei, Baoding, China) Kang, S.I. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: kangsu@gist.ac.kr Kim, S.I. (Seoul National University, Seoul, Republic of Korea) |
| description | A gene encoding inulin fructotransferase (di-D-fructofuranose 1,2':2,3' dianhydride [DFA Ⅲ]-producing IFTase, EC 4.2.2.18) from Bacillus sp. snu-7 was cloned. This gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a 410-amino acid mature protein. The deduced amino acid sequence was 98% identical to Arthrobacter globiformis C11-1 IFTase (DFA Ⅲ-producing). The enzyme was successfully expressed in E. coli as a functionally active, His-tagged protein, and it was purified in a single step using immobilized metal affinity chromatography. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69018098</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69018098</sourcerecordid><originalsourceid>FETCH-LOGICAL-f261t-60e64ce266aae458441cbdacd8863ddb3c8efc7957ba19e0f7ea3e0212b5ac993</originalsourceid><addsrcrecordid>eNpN0FFLwzAQB_A-KG5OP4KSF31aJUnbJH3Usak4EETBt3JNLzOSpTVpQf30VjbBp4Pjx__430EyZZTJVCpeTJLjGN8pFYwrcZRMmKIFywo2TV4XrvXWb-Zk-dkFjNG2fk7AN2TxBgF0j8F-Qz9uSWvIDWjr3BBJ7K5I9EMqyb0fnPVkFQbdt30AHw0GiHiSHBpwEU_3c5a8rJbPi7t0_Xh7v7hep4YL1qeCosg1ciEAMC9UnjNdN6AbpUTWNHWmFRoty0LWwEqkRiJkSDnjdQG6LLNZcrnL7UL7MWDsq62NGp0Dj-0QK1HSsW2pRni-h0O9xabqgt1C-Kr-fjGCiz2AqMGZsYu28Z_LJaPi9-LZzhloK9iE0Tw8cUolpRkvRfYDx0hwPw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69018098</pqid></control><display><type>article</type><title>Cloning, Expression, and Characterization of Bacillus sp. snu-7 Inulin Fructotransferase</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Kim, C.S. (Seoul National University, Seoul, Republic of Korea) ; Hong, C.K. (Seoul National University, Seoul, Republic of Korea) ; Kim, K.Y. (Seoul National University, Seoul, Republic of Korea) ; Wang, Xiu Ling (Agricultural University of Hebei, Baoding, China) ; Kang, S.I. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: kangsu@gist.ac.kr ; Kim, S.I. (Seoul National University, Seoul, Republic of Korea)</creator><creatorcontrib>Kim, C.S. (Seoul National University, Seoul, Republic of Korea) ; Hong, C.K. (Seoul National University, Seoul, Republic of Korea) ; Kim, K.Y. (Seoul National University, Seoul, Republic of Korea) ; Wang, Xiu Ling (Agricultural University of Hebei, Baoding, China) ; Kang, S.I. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: kangsu@gist.ac.kr ; Kim, S.I. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><description>A gene encoding inulin fructotransferase (di-D-fructofuranose 1,2':2,3' dianhydride [DFA Ⅲ]-producing IFTase, EC 4.2.2.18) from Bacillus sp. snu-7 was cloned. This gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a 410-amino acid mature protein. The deduced amino acid sequence was 98% identical to Arthrobacter globiformis C11-1 IFTase (DFA Ⅲ-producing). The enzyme was successfully expressed in E. coli as a functionally active, His-tagged protein, and it was purified in a single step using immobilized metal affinity chromatography.</description><identifier>ISSN: 1017-7825</identifier><identifier>PMID: 18051351</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>Amino Acid Sequence ; Bacillus - enzymology ; Bacillus - genetics ; Bacillus sp. snu-7 ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; difructofuranose anhydride ; DNA Primers - genetics ; DNA, Bacterial - genetics ; Enzyme Stability ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genes, Bacterial ; Hexosyltransferases - chemistry ; Hexosyltransferases - genetics ; Hexosyltransferases - metabolism ; Hydrogen-Ion Concentration ; Hydrolysis ; INULIN ; Inulin - metabolism ; inulin fructotransferase ; INULINA ; INULINE ; Molecular Sequence Data ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Sequence Homology, Amino Acid ; Temperature</subject><ispartof>Journal of microbiology and biotechnology, 2007-01, Vol.17 (1), p.37-43</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18471069$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18051351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, C.S. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Hong, C.K. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, K.Y. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Wang, Xiu Ling (Agricultural University of Hebei, Baoding, China)</creatorcontrib><creatorcontrib>Kang, S.I. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: kangsu@gist.ac.kr</creatorcontrib><creatorcontrib>Kim, S.I. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><title>Cloning, Expression, and Characterization of Bacillus sp. snu-7 Inulin Fructotransferase</title><title>Journal of microbiology and biotechnology</title><addtitle>J Microbiol Biotechnol</addtitle><description>A gene encoding inulin fructotransferase (di-D-fructofuranose 1,2':2,3' dianhydride [DFA Ⅲ]-producing IFTase, EC 4.2.2.18) from Bacillus sp. snu-7 was cloned. This gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a 410-amino acid mature protein. The deduced amino acid sequence was 98% identical to Arthrobacter globiformis C11-1 IFTase (DFA Ⅲ-producing). The enzyme was successfully expressed in E. coli as a functionally active, His-tagged protein, and it was purified in a single step using immobilized metal affinity chromatography.</description><subject>Amino Acid Sequence</subject><subject>Bacillus - enzymology</subject><subject>Bacillus - genetics</subject><subject>Bacillus sp. snu-7</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>difructofuranose anhydride</subject><subject>DNA Primers - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>Enzyme Stability</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes, Bacterial</subject><subject>Hexosyltransferases - chemistry</subject><subject>Hexosyltransferases - genetics</subject><subject>Hexosyltransferases - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>INULIN</subject><subject>Inulin - metabolism</subject><subject>inulin fructotransferase</subject><subject>INULINA</subject><subject>INULINE</subject><subject>Molecular Sequence Data</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Temperature</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0FFLwzAQB_A-KG5OP4KSF31aJUnbJH3Usak4EETBt3JNLzOSpTVpQf30VjbBp4Pjx__430EyZZTJVCpeTJLjGN8pFYwrcZRMmKIFywo2TV4XrvXWb-Zk-dkFjNG2fk7AN2TxBgF0j8F-Qz9uSWvIDWjr3BBJ7K5I9EMqyb0fnPVkFQbdt30AHw0GiHiSHBpwEU_3c5a8rJbPi7t0_Xh7v7hep4YL1qeCosg1ciEAMC9UnjNdN6AbpUTWNHWmFRoty0LWwEqkRiJkSDnjdQG6LLNZcrnL7UL7MWDsq62NGp0Dj-0QK1HSsW2pRni-h0O9xabqgt1C-Kr-fjGCiz2AqMGZsYu28Z_LJaPi9-LZzhloK9iE0Tw8cUolpRkvRfYDx0hwPw</recordid><startdate>200701</startdate><enddate>200701</enddate><creator>Kim, C.S. (Seoul National University, Seoul, Republic of Korea)</creator><creator>Hong, C.K. (Seoul National University, Seoul, Republic of Korea)</creator><creator>Kim, K.Y. (Seoul National University, Seoul, Republic of Korea)</creator><creator>Wang, Xiu Ling (Agricultural University of Hebei, Baoding, China)</creator><creator>Kang, S.I. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: kangsu@gist.ac.kr</creator><creator>Kim, S.I. (Seoul National University, Seoul, Republic of Korea)</creator><general>Korean Society for Applied Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200701</creationdate><title>Cloning, Expression, and Characterization of Bacillus sp. snu-7 Inulin Fructotransferase</title><author>Kim, C.S. (Seoul National University, Seoul, Republic of Korea) ; Hong, C.K. (Seoul National University, Seoul, Republic of Korea) ; Kim, K.Y. (Seoul National University, Seoul, Republic of Korea) ; Wang, Xiu Ling (Agricultural University of Hebei, Baoding, China) ; Kang, S.I. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: kangsu@gist.ac.kr ; Kim, S.I. (Seoul National University, Seoul, Republic of Korea)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f261t-60e64ce266aae458441cbdacd8863ddb3c8efc7957ba19e0f7ea3e0212b5ac993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Bacillus - enzymology</topic><topic>Bacillus - genetics</topic><topic>Bacillus sp. snu-7</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>difructofuranose anhydride</topic><topic>DNA Primers - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>Enzyme Stability</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes, Bacterial</topic><topic>Hexosyltransferases - chemistry</topic><topic>Hexosyltransferases - genetics</topic><topic>Hexosyltransferases - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>INULIN</topic><topic>Inulin - metabolism</topic><topic>inulin fructotransferase</topic><topic>INULINA</topic><topic>INULINE</topic><topic>Molecular Sequence Data</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, C.S. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Hong, C.K. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, K.Y. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><creatorcontrib>Wang, Xiu Ling (Agricultural University of Hebei, Baoding, China)</creatorcontrib><creatorcontrib>Kang, S.I. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: kangsu@gist.ac.kr</creatorcontrib><creatorcontrib>Kim, S.I. (Seoul National University, Seoul, Republic of Korea)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, C.S. (Seoul National University, Seoul, Republic of Korea)</au><au>Hong, C.K. (Seoul National University, Seoul, Republic of Korea)</au><au>Kim, K.Y. (Seoul National University, Seoul, Republic of Korea)</au><au>Wang, Xiu Ling (Agricultural University of Hebei, Baoding, China)</au><au>Kang, S.I. (Gwangju Institute of Science and Technology, Gwangju, Republic of Korea), E-mail: kangsu@gist.ac.kr</au><au>Kim, S.I. (Seoul National University, Seoul, Republic of Korea)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, Expression, and Characterization of Bacillus sp. snu-7 Inulin Fructotransferase</atitle><jtitle>Journal of microbiology and biotechnology</jtitle><addtitle>J Microbiol Biotechnol</addtitle><date>2007-01</date><risdate>2007</risdate><volume>17</volume><issue>1</issue><spage>37</spage><epage>43</epage><pages>37-43</pages><issn>1017-7825</issn><abstract>A gene encoding inulin fructotransferase (di-D-fructofuranose 1,2':2,3' dianhydride [DFA Ⅲ]-producing IFTase, EC 4.2.2.18) from Bacillus sp. snu-7 was cloned. This gene was composed of a single, 1,353-bp open reading frame encoding a protein composed of a 40-amino acid signal peptide and a 410-amino acid mature protein. The deduced amino acid sequence was 98% identical to Arthrobacter globiformis C11-1 IFTase (DFA Ⅲ-producing). The enzyme was successfully expressed in E. coli as a functionally active, His-tagged protein, and it was purified in a single step using immobilized metal affinity chromatography.</abstract><cop>Seoul</cop><pub>Korean Society for Applied Microbiology</pub><pmid>18051351</pmid><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1017-7825 |
| ispartof | Journal of microbiology and biotechnology, 2007-01, Vol.17 (1), p.37-43 |
| issn | 1017-7825 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69018098 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Bacillus - enzymology Bacillus - genetics Bacillus sp. snu-7 Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular difructofuranose anhydride DNA Primers - genetics DNA, Bacterial - genetics Enzyme Stability Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genes, Bacterial Hexosyltransferases - chemistry Hexosyltransferases - genetics Hexosyltransferases - metabolism Hydrogen-Ion Concentration Hydrolysis INULIN Inulin - metabolism inulin fructotransferase INULINA INULINE Molecular Sequence Data Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Homology, Amino Acid Temperature |
| title | Cloning, Expression, and Characterization of Bacillus sp. snu-7 Inulin Fructotransferase |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T06%3A45%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning,%20Expression,%20and%20Characterization%20of%20Bacillus%20sp.%20snu-7%20Inulin%20Fructotransferase&rft.jtitle=Journal%20of%20microbiology%20and%20biotechnology&rft.au=Kim,%20C.S.%20(Seoul%20National%20University,%20Seoul,%20Republic%20of%20Korea)&rft.date=2007-01&rft.volume=17&rft.issue=1&rft.spage=37&rft.epage=43&rft.pages=37-43&rft.issn=1017-7825&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E69018098%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69018098&rft_id=info:pmid/18051351&rfr_iscdi=true |