Chemically Induced Expression of Rice OSB2 under the Control of the OsPR1.1 Promoter Confers Increased Anthocyanin Accumulation in Transgenic Rice
Anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. In this study, OsPR1.1 promoter was isolated and the promoter activity was monitored using a reporter gene OSB2, which encodes a transcription factor for anthocyanin synthesi...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2007-02, Vol.55 (4), p.1241-1247 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1247 |
|---|---|
| container_issue | 4 |
| container_start_page | 1241 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 55 |
| creator | Kawahigashi, Hiroyuki Hirose, Sakiko Iwai, Takayoshi Ohashi, Yuko Sakamoto, Wataru Maekawa, Masahiko Ohkawa, Yasunobu |
| description | Anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. In this study, OsPR1.1 promoter was isolated and the promoter activity was monitored using a reporter gene OSB2, which encodes a transcription factor for anthocyanin synthesis in rice plants. We introduced PR::OSB2 plasmid into an isogenic Taichung 65, no. 99-962 T-65 CBA B9F5 (T65 CBA), rice line (Oryza sativa L.) and found that the transgenic rice plants exhibited anthocyanin accumulation by the induced expression of OSB2 after chemical treatments with methyl jasmonate (MeJA) and 2,6-dichloroisonicotinic acid (DCINA). The shoots of the PR::OSB2 transgenic rice plants changed color to red after application of the chemicals accompanying with the increased anthocyanin content to approximately 5-fold by MeJA and 2-fold by DCINA, respectively. The anthocyanin accumulation was consistent with the increase of the expression of OSB2 and anthocyanidin synthase (ANS). This color change system could provide a useful and easy way to produce transgenic plants for monitoring of chemicals in the environment. Keywords: Anthocyanidin synthase; biomonitoring; 2,6-dichloroisonicotinic acid (DCINA); methyl jasmonate; Oryza sativa; phenylalanine ammonia lyase |
| doi_str_mv | 10.1021/jf062339w |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69012494</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69012494</sourcerecordid><originalsourceid>FETCH-LOGICAL-a502t-43966aae107016c61b2d2df216a0f6491aae3f20decc5cbee9d4765e5e3e58c73</originalsourceid><addsrcrecordid>eNqFkUFvEzEQhVcIREPhwB8AX4rEYYvHu7Z3j2koUKlSQpP20IvleGebDbt2sHdF8zf4xTgkai5InEaj9-k9zbwkeQv0HCiDT-uaCpZl5a9nyQg4oykHKJ4nIxrFtOACTpJXIawppQWX9GVyApLxTAIdJb8nK-wao9t2S65sNRisyOXjxmMIjbPE1eSmMUim8wtGBluhJ_0KycTZ3rt2J-_WaZjdwDmQmXed6yMT9Rp9iI7Gow7Rc2z7lTNbbRtLxsYM3dDqfpcQ94XXNjygbczfsNfJi1q3Ad8c5mly--VyMfmWXk-_Xk3G16nmlPVpnpVCaI1AJQVhBCxZxaqagdC0FnkJUctqRis0hpslYlnlUnDkmCEvjMxOkw973413PwcMveqaYLBttUU3BCVKCiwv8_-CUHJWAi8i-HEPGu9C8FirjW867bcKqNo1pZ6aiuy7g-mw7LA6kodqInB2AHSI_dTxSaYJR67gkZO7M9I914QeH5907X8oITPJ1WI2V5_lHft-cXev7iP_fs_X2in94KPn7ZxRyCiVeVFIfkzWJqi1G7yNNfzjhD-t_MBH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19529158</pqid></control><display><type>article</type><title>Chemically Induced Expression of Rice OSB2 under the Control of the OsPR1.1 Promoter Confers Increased Anthocyanin Accumulation in Transgenic Rice</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Kawahigashi, Hiroyuki ; Hirose, Sakiko ; Iwai, Takayoshi ; Ohashi, Yuko ; Sakamoto, Wataru ; Maekawa, Masahiko ; Ohkawa, Yasunobu</creator><creatorcontrib>Kawahigashi, Hiroyuki ; Hirose, Sakiko ; Iwai, Takayoshi ; Ohashi, Yuko ; Sakamoto, Wataru ; Maekawa, Masahiko ; Ohkawa, Yasunobu</creatorcontrib><description>Anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. In this study, OsPR1.1 promoter was isolated and the promoter activity was monitored using a reporter gene OSB2, which encodes a transcription factor for anthocyanin synthesis in rice plants. We introduced PR::OSB2 plasmid into an isogenic Taichung 65, no. 99-962 T-65 CBA B9F5 (T65 CBA), rice line (Oryza sativa L.) and found that the transgenic rice plants exhibited anthocyanin accumulation by the induced expression of OSB2 after chemical treatments with methyl jasmonate (MeJA) and 2,6-dichloroisonicotinic acid (DCINA). The shoots of the PR::OSB2 transgenic rice plants changed color to red after application of the chemicals accompanying with the increased anthocyanin content to approximately 5-fold by MeJA and 2-fold by DCINA, respectively. The anthocyanin accumulation was consistent with the increase of the expression of OSB2 and anthocyanidin synthase (ANS). This color change system could provide a useful and easy way to produce transgenic plants for monitoring of chemicals in the environment. Keywords: Anthocyanidin synthase; biomonitoring; 2,6-dichloroisonicotinic acid (DCINA); methyl jasmonate; Oryza sativa; phenylalanine ammonia lyase</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf062339w</identifier><identifier>PMID: 17253710</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>2,6-dichloroisonicotinic acid ; Acetates - pharmacology ; anthocyanidin synthase ; anthocyanins ; Anthocyanins - biosynthesis ; Anthocyanins - genetics ; Basic Helix-Loop-Helix Transcription Factors - genetics ; beta-glucuronidase ; Biological and medical sciences ; biosynthesis ; Cereal and baking product industries ; color ; Cyclopentanes - pharmacology ; Food industries ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression - drug effects ; gene induction ; genes ; Isonicotinic Acids - pharmacology ; leaves ; methyl jasmonate ; Oryza - genetics ; Oryza - metabolism ; Oryza sativa ; OsPR1.1 promoter ; Oxylipins ; Plant Proteins - genetics ; Plants, Genetically Modified - metabolism ; promoter regions ; Promoter Regions, Genetic - genetics ; reporter genes ; rice ; stems ; transcription factors ; transgenic plants</subject><ispartof>Journal of agricultural and food chemistry, 2007-02, Vol.55 (4), p.1241-1247</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a502t-43966aae107016c61b2d2df216a0f6491aae3f20decc5cbee9d4765e5e3e58c73</citedby><cites>FETCH-LOGICAL-a502t-43966aae107016c61b2d2df216a0f6491aae3f20decc5cbee9d4765e5e3e58c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf062339w$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf062339w$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27074,27922,27923,56736,56786</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18553777$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17253710$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawahigashi, Hiroyuki</creatorcontrib><creatorcontrib>Hirose, Sakiko</creatorcontrib><creatorcontrib>Iwai, Takayoshi</creatorcontrib><creatorcontrib>Ohashi, Yuko</creatorcontrib><creatorcontrib>Sakamoto, Wataru</creatorcontrib><creatorcontrib>Maekawa, Masahiko</creatorcontrib><creatorcontrib>Ohkawa, Yasunobu</creatorcontrib><title>Chemically Induced Expression of Rice OSB2 under the Control of the OsPR1.1 Promoter Confers Increased Anthocyanin Accumulation in Transgenic Rice</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. In this study, OsPR1.1 promoter was isolated and the promoter activity was monitored using a reporter gene OSB2, which encodes a transcription factor for anthocyanin synthesis in rice plants. We introduced PR::OSB2 plasmid into an isogenic Taichung 65, no. 99-962 T-65 CBA B9F5 (T65 CBA), rice line (Oryza sativa L.) and found that the transgenic rice plants exhibited anthocyanin accumulation by the induced expression of OSB2 after chemical treatments with methyl jasmonate (MeJA) and 2,6-dichloroisonicotinic acid (DCINA). The shoots of the PR::OSB2 transgenic rice plants changed color to red after application of the chemicals accompanying with the increased anthocyanin content to approximately 5-fold by MeJA and 2-fold by DCINA, respectively. The anthocyanin accumulation was consistent with the increase of the expression of OSB2 and anthocyanidin synthase (ANS). This color change system could provide a useful and easy way to produce transgenic plants for monitoring of chemicals in the environment. Keywords: Anthocyanidin synthase; biomonitoring; 2,6-dichloroisonicotinic acid (DCINA); methyl jasmonate; Oryza sativa; phenylalanine ammonia lyase</description><subject>2,6-dichloroisonicotinic acid</subject><subject>Acetates - pharmacology</subject><subject>anthocyanidin synthase</subject><subject>anthocyanins</subject><subject>Anthocyanins - biosynthesis</subject><subject>Anthocyanins - genetics</subject><subject>Basic Helix-Loop-Helix Transcription Factors - genetics</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>Cereal and baking product industries</subject><subject>color</subject><subject>Cyclopentanes - pharmacology</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression - drug effects</subject><subject>gene induction</subject><subject>genes</subject><subject>Isonicotinic Acids - pharmacology</subject><subject>leaves</subject><subject>methyl jasmonate</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>Oryza sativa</subject><subject>OsPR1.1 promoter</subject><subject>Oxylipins</subject><subject>Plant Proteins - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>reporter genes</subject><subject>rice</subject><subject>stems</subject><subject>transcription factors</subject><subject>transgenic plants</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvEzEQhVcIREPhwB8AX4rEYYvHu7Z3j2koUKlSQpP20IvleGebDbt2sHdF8zf4xTgkai5InEaj9-k9zbwkeQv0HCiDT-uaCpZl5a9nyQg4oykHKJ4nIxrFtOACTpJXIawppQWX9GVyApLxTAIdJb8nK-wao9t2S65sNRisyOXjxmMIjbPE1eSmMUim8wtGBluhJ_0KycTZ3rt2J-_WaZjdwDmQmXed6yMT9Rp9iI7Gow7Rc2z7lTNbbRtLxsYM3dDqfpcQ94XXNjygbczfsNfJi1q3Ad8c5mly--VyMfmWXk-_Xk3G16nmlPVpnpVCaI1AJQVhBCxZxaqagdC0FnkJUctqRis0hpslYlnlUnDkmCEvjMxOkw973413PwcMveqaYLBttUU3BCVKCiwv8_-CUHJWAi8i-HEPGu9C8FirjW867bcKqNo1pZ6aiuy7g-mw7LA6kodqInB2AHSI_dTxSaYJR67gkZO7M9I914QeH5907X8oITPJ1WI2V5_lHft-cXev7iP_fs_X2in94KPn7ZxRyCiVeVFIfkzWJqi1G7yNNfzjhD-t_MBH</recordid><startdate>20070221</startdate><enddate>20070221</enddate><creator>Kawahigashi, Hiroyuki</creator><creator>Hirose, Sakiko</creator><creator>Iwai, Takayoshi</creator><creator>Ohashi, Yuko</creator><creator>Sakamoto, Wataru</creator><creator>Maekawa, Masahiko</creator><creator>Ohkawa, Yasunobu</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070221</creationdate><title>Chemically Induced Expression of Rice OSB2 under the Control of the OsPR1.1 Promoter Confers Increased Anthocyanin Accumulation in Transgenic Rice</title><author>Kawahigashi, Hiroyuki ; Hirose, Sakiko ; Iwai, Takayoshi ; Ohashi, Yuko ; Sakamoto, Wataru ; Maekawa, Masahiko ; Ohkawa, Yasunobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a502t-43966aae107016c61b2d2df216a0f6491aae3f20decc5cbee9d4765e5e3e58c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>2,6-dichloroisonicotinic acid</topic><topic>Acetates - pharmacology</topic><topic>anthocyanidin synthase</topic><topic>anthocyanins</topic><topic>Anthocyanins - biosynthesis</topic><topic>Anthocyanins - genetics</topic><topic>Basic Helix-Loop-Helix Transcription Factors - genetics</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>Cereal and baking product industries</topic><topic>color</topic><topic>Cyclopentanes - pharmacology</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression - drug effects</topic><topic>gene induction</topic><topic>genes</topic><topic>Isonicotinic Acids - pharmacology</topic><topic>leaves</topic><topic>methyl jasmonate</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>Oryza sativa</topic><topic>OsPR1.1 promoter</topic><topic>Oxylipins</topic><topic>Plant Proteins - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>reporter genes</topic><topic>rice</topic><topic>stems</topic><topic>transcription factors</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawahigashi, Hiroyuki</creatorcontrib><creatorcontrib>Hirose, Sakiko</creatorcontrib><creatorcontrib>Iwai, Takayoshi</creatorcontrib><creatorcontrib>Ohashi, Yuko</creatorcontrib><creatorcontrib>Sakamoto, Wataru</creatorcontrib><creatorcontrib>Maekawa, Masahiko</creatorcontrib><creatorcontrib>Ohkawa, Yasunobu</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawahigashi, Hiroyuki</au><au>Hirose, Sakiko</au><au>Iwai, Takayoshi</au><au>Ohashi, Yuko</au><au>Sakamoto, Wataru</au><au>Maekawa, Masahiko</au><au>Ohkawa, Yasunobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemically Induced Expression of Rice OSB2 under the Control of the OsPR1.1 Promoter Confers Increased Anthocyanin Accumulation in Transgenic Rice</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2007-02-21</date><risdate>2007</risdate><volume>55</volume><issue>4</issue><spage>1241</spage><epage>1247</epage><pages>1241-1247</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. In this study, OsPR1.1 promoter was isolated and the promoter activity was monitored using a reporter gene OSB2, which encodes a transcription factor for anthocyanin synthesis in rice plants. We introduced PR::OSB2 plasmid into an isogenic Taichung 65, no. 99-962 T-65 CBA B9F5 (T65 CBA), rice line (Oryza sativa L.) and found that the transgenic rice plants exhibited anthocyanin accumulation by the induced expression of OSB2 after chemical treatments with methyl jasmonate (MeJA) and 2,6-dichloroisonicotinic acid (DCINA). The shoots of the PR::OSB2 transgenic rice plants changed color to red after application of the chemicals accompanying with the increased anthocyanin content to approximately 5-fold by MeJA and 2-fold by DCINA, respectively. The anthocyanin accumulation was consistent with the increase of the expression of OSB2 and anthocyanidin synthase (ANS). This color change system could provide a useful and easy way to produce transgenic plants for monitoring of chemicals in the environment. Keywords: Anthocyanidin synthase; biomonitoring; 2,6-dichloroisonicotinic acid (DCINA); methyl jasmonate; Oryza sativa; phenylalanine ammonia lyase</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17253710</pmid><doi>10.1021/jf062339w</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2007-02, Vol.55 (4), p.1241-1247 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69012494 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | 2,6-dichloroisonicotinic acid Acetates - pharmacology anthocyanidin synthase anthocyanins Anthocyanins - biosynthesis Anthocyanins - genetics Basic Helix-Loop-Helix Transcription Factors - genetics beta-glucuronidase Biological and medical sciences biosynthesis Cereal and baking product industries color Cyclopentanes - pharmacology Food industries Fundamental and applied biological sciences. Psychology gene expression Gene Expression - drug effects gene induction genes Isonicotinic Acids - pharmacology leaves methyl jasmonate Oryza - genetics Oryza - metabolism Oryza sativa OsPR1.1 promoter Oxylipins Plant Proteins - genetics Plants, Genetically Modified - metabolism promoter regions Promoter Regions, Genetic - genetics reporter genes rice stems transcription factors transgenic plants |
| title | Chemically Induced Expression of Rice OSB2 under the Control of the OsPR1.1 Promoter Confers Increased Anthocyanin Accumulation in Transgenic Rice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T05%3A56%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chemically%20Induced%20Expression%20of%20Rice%20OSB2%20under%20the%20Control%20of%20the%20OsPR1.1%20Promoter%20Confers%20Increased%20Anthocyanin%20Accumulation%20in%20Transgenic%20Rice&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Kawahigashi,%20Hiroyuki&rft.date=2007-02-21&rft.volume=55&rft.issue=4&rft.spage=1241&rft.epage=1247&rft.pages=1241-1247&rft.issn=0021-8561&rft.eissn=1520-5118&rft.coden=JAFCAU&rft_id=info:doi/10.1021/jf062339w&rft_dat=%3Cproquest_cross%3E69012494%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19529158&rft_id=info:pmid/17253710&rfr_iscdi=true |