Factors affecting iron sulfide-enhanced bacteriophage plaque assays in Salmonella

Reaction of ferric ions with hydrogen sulfide (H 2S) enhances contrast of phage plaques in H 2S+ Salmonella, but contrast diminishes in weak H 2S+ strains. H 2S was affected by concentrations of peptones, glucose, ferric ammonium citrate (FAC) and sodium thiosulfate (ST), and by FAC:ST ratio, temper...

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Veröffentlicht in:	Journal of microbiological methods 2006-12, Vol.67 (3), p.611-615
1. Verfasser:	McLaughlin, M.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Bacteriophage Biological and medical sciences Culture Media - chemistry Fundamental and applied biological sciences. Psychology Hydrogen sulfide Hydrogen Sulfide - metabolism Hydrogen-Ion Concentration Microbiology Plaque assay Salmonella Salmonella - metabolism Salmonella - virology Salmonella Phages - growth & development Temperature Viral Plaque Assay - methods
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Zusammenfassung:	Reaction of ferric ions with hydrogen sulfide (H 2S) enhances contrast of phage plaques in H 2S+ Salmonella, but contrast diminishes in weak H 2S+ strains. H 2S was affected by concentrations of peptones, glucose, ferric ammonium citrate (FAC) and sodium thiosulfate (ST), and by FAC:ST ratio, temperature, pH, air, and host strain. Increasing peptone levels was most important for improving contrast in weak H 2S+ strains.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2006.05.015