Isolation of a novel mycovirus OMIV in Pleurotus ostreatus and its detection using a triple antibody sandwich-ELISA

A novel mycovirus was isolated from a diseased mushroom, Pleurotus ostreatus, using a purification procedure involving polyethylene glycol (PEG)–NaCl precipitation, differential centrifugation, and equilibrium centrifugation in a CsCl gradient. The virion was a 43 nm isometric virus encapsulating do...

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Veröffentlicht in:	Journal of virological methods 2006-12, Vol.138 (1), p.24-29
Hauptverfasser:	Ro, H.S., Lee, N.J., Lee, C.W., Lee, H.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Viral - immunology Biological and medical sciences Centrifugation Centrifugation, Density Gradient Chemical Precipitation Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Genome, Viral Microbiology Microscopy, Electron, Transmission Mycological methods and techniques used in mycology Mycology Mycovirus OMIV OMSV Pleurotus - virology Pleurotus ostreatus Polyethylene Glycols RNA Viruses - genetics RNA Viruses - immunology RNA Viruses - isolation & purification RNA Viruses - ultrastructure RNA, Double-Stranded - analysis RNA, Viral - analysis Sensitivity and Specificity Sodium Chloride TAS-ELISA Techniques used in virology Virion - ultrastructure Virology
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