High-Yielding Enzymatic α-Glucosylation of Pyridoxine by Marine α-Glucosidase from Aplysia fasciata
We recently succeeded in the identification and purification of an interesting marine exo-α-glucosidase (EC 3.2.1.20) from the anaspidean mollusc Aplysia fasciata. The enzyme was characterized by good transglycosylation activity toward different acceptors using maltose as donor. High-yielding enzyma...
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| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2006-09, Vol.8 (5), p.448-452 |
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| container_title | Marine biotechnology (New York, N.Y.) |
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| creator | Tramice, Annabella Giordano, Assunta Andreotti, Giuseppina Mollo, Ernesto Trincone, Antonio |
| description | We recently succeeded in the identification and purification of an interesting marine exo-α-glucosidase (EC 3.2.1.20) from the anaspidean mollusc Aplysia fasciata. The enzyme was characterized by good transglycosylation activity toward different acceptors using maltose as donor. High-yielding enzymatic α-glycosylation of pyridoxine using this marine enzyme is reported here; the reaction has been optimized, reaching 80% molar yield of products (pyridoxine monoglucosides 24 g/l; pyridoxine isomaltoside 35 g/l). High selectivity toward the 5′ position is observed for both monoglucoside and disaccharide formation. This is the first report describing the enzymatic production of pyridoxine isomaltoside. |
| doi_str_mv | 10.1007/s10126-005-6144-4 |
| format | Article |
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The enzyme was characterized by good transglycosylation activity toward different acceptors using maltose as donor. High-yielding enzymatic α-glycosylation of pyridoxine using this marine enzyme is reported here; the reaction has been optimized, reaching 80% molar yield of products (pyridoxine monoglucosides 24 g/l; pyridoxine isomaltoside 35 g/l). High selectivity toward the 5′ position is observed for both monoglucoside and disaccharide formation. 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This is the first report describing the enzymatic production of pyridoxine isomaltoside.</description><subject>alpha-Glucosidases - metabolism</subject><subject>Animals</subject><subject>Glycosylation</subject><subject>Molecular Structure</subject><subject>Mollusca - enzymology</subject><subject>Pyridoxine - chemistry</subject><subject>Pyridoxine - metabolism</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFO3DAURS0EAjr0A7opXnXn8p7jeJIlQhSQqIoEs-jKeontqaskHuwZifSv-BG-qRnNCFbvPuncuziMfUH4jgDzi4yAUguAUmhUSqgDdoqq0ELKQh--Z1mdsE85_4WpMy_gmJ2g1nOodX3K3G1Y_hG_g-tsGJb8evg39rQOLX97FTfdpo157KY_Djx6_jCmYONLGBxvRv6T0ja9g8FSdtyn2PPLVTfmQNxTbgOt6Ywdeeqy-7y_M7b4cf10dSvuf93cXV3ei7Yo5VqQrUtvG11j5VRh5yARS7JWeSWllqquXWOl176CxldYaqeoKqGpS5pq5IoZ-7bbXaX4vHF5bfqQW9d1NLi4yUbXgKCgmkDcgW2KOSfnzSqFntJoEMzWrdm5NZNbs3Vr1NT5uh_fNL2zH429zAk43wGeoqFlCtksHiWgBkClNULxH4qIgHI</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Tramice, Annabella</creator><creator>Giordano, Assunta</creator><creator>Andreotti, Giuseppina</creator><creator>Mollo, Ernesto</creator><creator>Trincone, Antonio</creator><general>Springer-Verlag</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>High-Yielding Enzymatic α-Glucosylation of Pyridoxine by Marine α-Glucosidase from Aplysia fasciata</title><author>Tramice, Annabella ; Giordano, Assunta ; Andreotti, Giuseppina ; Mollo, Ernesto ; Trincone, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-ad95fdb6918e43d702115add4f42262499ebd2f6f80bf8156e4a850b95afdbae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>alpha-Glucosidases - metabolism</topic><topic>Animals</topic><topic>Glycosylation</topic><topic>Molecular Structure</topic><topic>Mollusca - enzymology</topic><topic>Pyridoxine - chemistry</topic><topic>Pyridoxine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tramice, Annabella</creatorcontrib><creatorcontrib>Giordano, Assunta</creatorcontrib><creatorcontrib>Andreotti, Giuseppina</creatorcontrib><creatorcontrib>Mollo, Ernesto</creatorcontrib><creatorcontrib>Trincone, Antonio</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Marine biotechnology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tramice, Annabella</au><au>Giordano, Assunta</au><au>Andreotti, Giuseppina</au><au>Mollo, Ernesto</au><au>Trincone, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Yielding Enzymatic α-Glucosylation of Pyridoxine by Marine α-Glucosidase from Aplysia fasciata</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>8</volume><issue>5</issue><spage>448</spage><epage>452</epage><pages>448-452</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>We recently succeeded in the identification and purification of an interesting marine exo-α-glucosidase (EC 3.2.1.20) from the anaspidean mollusc Aplysia fasciata. 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| identifier | ISSN: 1436-2228 |
| ispartof | Marine biotechnology (New York, N.Y.), 2006-09, Vol.8 (5), p.448-452 |
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| language | eng |
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| source | MEDLINE; Springer Nature |
| subjects | alpha-Glucosidases - metabolism Animals Glycosylation Molecular Structure Mollusca - enzymology Pyridoxine - chemistry Pyridoxine - metabolism |
| title | High-Yielding Enzymatic α-Glucosylation of Pyridoxine by Marine α-Glucosidase from Aplysia fasciata |
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