Evaluation of nanoparticles loaded with benzopsoralen in rat peritoneal exudate cells
Psoralens are widely used for the treatment of hyperproliferative skin disease. In this work, we prepared nanoparticles (NP) containing a benzopsoralen (3-ethoxy carbonyl-2 H-benzofuro[3,2- f]-1-benzopiran-2-one) by the solvent evaporation technique. We evaluated important NP parameters such as part...
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| Veröffentlicht in: | International journal of pharmaceutics 2007-03, Vol.332 (1), p.153-160 |
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| creator | Gomes, A.J. Faustino, A.S. Lunardi, C.N. Lunardi, L.O. Machado, A.E.H. |
| description | Psoralens are widely used for the treatment of hyperproliferative skin disease. In this work, we prepared nanoparticles (NP) containing a benzopsoralen (3-ethoxy carbonyl-2
H-benzofuro[3,2-
f]-1-benzopiran-2-one) by the solvent evaporation technique. We evaluated important NP parameters such as particle size, drug encapsulation efficiency, effect of the encapsulation process over the drug's photochemistry, zeta potential, external morphology, and
in vitro release behavior. We also investigated the nanoparticle as a drug delivery system (DDS), as well as its target delivery to the action site, which is a very important parameter to increase the therapeutic use of psoralens and to reduce their side effects. The uptake of benzopsoralen-loaded PLGA nanoparticles by different kinds of cells found in rat peritoneal exudates was also studied. The photodamage promoted by irradiation with UV light revealed morphological characteristics of cell damage such as cytoplasmic vesiculation, mitochondrial damage, and swelling of both the granular endoplasmatic reticulum and nuclear membrane. This encapsulation method maintained the drug's properties and improved drug delivery to the target cell. |
| doi_str_mv | 10.1016/j.ijpharm.2006.09.035 |
| format | Article |
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H-benzofuro[3,2-
f]-1-benzopiran-2-one) by the solvent evaporation technique. We evaluated important NP parameters such as particle size, drug encapsulation efficiency, effect of the encapsulation process over the drug's photochemistry, zeta potential, external morphology, and
in vitro release behavior. We also investigated the nanoparticle as a drug delivery system (DDS), as well as its target delivery to the action site, which is a very important parameter to increase the therapeutic use of psoralens and to reduce their side effects. The uptake of benzopsoralen-loaded PLGA nanoparticles by different kinds of cells found in rat peritoneal exudates was also studied. The photodamage promoted by irradiation with UV light revealed morphological characteristics of cell damage such as cytoplasmic vesiculation, mitochondrial damage, and swelling of both the granular endoplasmatic reticulum and nuclear membrane. This encapsulation method maintained the drug's properties and improved drug delivery to the target cell.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2006.09.035</identifier><identifier>PMID: 17056212</identifier><identifier>CODEN: IJPHDE</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Ascitic Fluid - cytology ; Ascitic Fluid - drug effects ; Ascitic Fluid - metabolism ; Benzopsoralen ; Biological and medical sciences ; Chemistry, Pharmaceutical ; Dermatologic Agents - chemistry ; Dermatologic Agents - metabolism ; Dermatologic Agents - pharmacology ; Dermatologic Agents - radiation effects ; Drug Carriers ; Drug Compounding ; Endocytosis ; Exudates peritoneal cell ; Furocoumarins - chemistry ; Furocoumarins - metabolism ; Furocoumarins - pharmacology ; Furocoumarins - radiation effects ; General pharmacology ; In Vitro Techniques ; Kinetics ; Lactic Acid - chemistry ; Male ; Medical sciences ; Nanoparticle ; Nanoparticles ; Particle Size ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Photosensitizing Agents - chemistry ; Photosensitizing Agents - metabolism ; Photosensitizing Agents - pharmacology ; Photosensitizing Agents - radiation effects ; Polyglycolic Acid - chemistry ; Polymers - chemistry ; PUVA ; PUVA Therapy ; Rats ; Rats, Wistar ; Solubility ; Surface Properties ; Technology, Pharmaceutical - methods ; Ultraviolet Rays</subject><ispartof>International journal of pharmaceutics, 2007-03, Vol.332 (1), p.153-160</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-75494629e804d3900179dd0ad68909e0d54cc285de0859f18e7b0e36473912713</citedby><cites>FETCH-LOGICAL-c393t-75494629e804d3900179dd0ad68909e0d54cc285de0859f18e7b0e36473912713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378517306007915$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18550839$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17056212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gomes, A.J.</creatorcontrib><creatorcontrib>Faustino, A.S.</creatorcontrib><creatorcontrib>Lunardi, C.N.</creatorcontrib><creatorcontrib>Lunardi, L.O.</creatorcontrib><creatorcontrib>Machado, A.E.H.</creatorcontrib><title>Evaluation of nanoparticles loaded with benzopsoralen in rat peritoneal exudate cells</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>Psoralens are widely used for the treatment of hyperproliferative skin disease. In this work, we prepared nanoparticles (NP) containing a benzopsoralen (3-ethoxy carbonyl-2
H-benzofuro[3,2-
f]-1-benzopiran-2-one) by the solvent evaporation technique. We evaluated important NP parameters such as particle size, drug encapsulation efficiency, effect of the encapsulation process over the drug's photochemistry, zeta potential, external morphology, and
in vitro release behavior. We also investigated the nanoparticle as a drug delivery system (DDS), as well as its target delivery to the action site, which is a very important parameter to increase the therapeutic use of psoralens and to reduce their side effects. The uptake of benzopsoralen-loaded PLGA nanoparticles by different kinds of cells found in rat peritoneal exudates was also studied. The photodamage promoted by irradiation with UV light revealed morphological characteristics of cell damage such as cytoplasmic vesiculation, mitochondrial damage, and swelling of both the granular endoplasmatic reticulum and nuclear membrane. This encapsulation method maintained the drug's properties and improved drug delivery to the target cell.</description><subject>Animals</subject><subject>Ascitic Fluid - cytology</subject><subject>Ascitic Fluid - drug effects</subject><subject>Ascitic Fluid - metabolism</subject><subject>Benzopsoralen</subject><subject>Biological and medical sciences</subject><subject>Chemistry, Pharmaceutical</subject><subject>Dermatologic Agents - chemistry</subject><subject>Dermatologic Agents - metabolism</subject><subject>Dermatologic Agents - pharmacology</subject><subject>Dermatologic Agents - radiation effects</subject><subject>Drug Carriers</subject><subject>Drug Compounding</subject><subject>Endocytosis</subject><subject>Exudates peritoneal cell</subject><subject>Furocoumarins - chemistry</subject><subject>Furocoumarins - metabolism</subject><subject>Furocoumarins - pharmacology</subject><subject>Furocoumarins - radiation effects</subject><subject>General pharmacology</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Lactic Acid - chemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nanoparticle</subject><subject>Nanoparticles</subject><subject>Particle Size</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Photosensitizing Agents - chemistry</subject><subject>Photosensitizing Agents - metabolism</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Photosensitizing Agents - radiation effects</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Polymers - chemistry</subject><subject>PUVA</subject><subject>PUVA Therapy</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Solubility</subject><subject>Surface Properties</subject><subject>Technology, Pharmaceutical - methods</subject><subject>Ultraviolet Rays</subject><issn>0378-5173</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAQgC0EosvCTwD5AreEcRzH8QmhqjykSlzo2fLaE9Urrx3spFB-PV5tpB65zMzhm9dHyFsGLQM2fDy2_jjfm3xqO4ChBdUCF8_Ijo2SN7yXw3OyAy7HRjDJr8irUo5QwY7xl-SKSRC17Hbk7ubBhNUsPkWaJhpNTLPJi7cBCw3JOHT0t1_u6QHj3zSXlE3ASH2k2Sx0xuyXFNEEin9WZxakFkMor8mLyYSCb7a8J3dfbn5ef2tuf3z9fv35trFc8aWRolf90CkcoXdcATCpnAPjhlGBQnCit7YbhUMYhZrYiPIAyIdecsU6yfiefLjMnXP6tWJZ9MmX8wUmYlqLHupMVUMFxQW0OZWScdJz9ieTHzUDffapj3rzqc8-NShdfda-d9uC9XBC99S1CazA-w0wxZowZROtL0_cKASM9dk9-XThsOp48Jh1sR6jRecz2kW75P9zyj97jZYV</recordid><startdate>20070306</startdate><enddate>20070306</enddate><creator>Gomes, A.J.</creator><creator>Faustino, A.S.</creator><creator>Lunardi, C.N.</creator><creator>Lunardi, L.O.</creator><creator>Machado, A.E.H.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070306</creationdate><title>Evaluation of nanoparticles loaded with benzopsoralen in rat peritoneal exudate cells</title><author>Gomes, A.J. ; Faustino, A.S. ; Lunardi, C.N. ; Lunardi, L.O. ; Machado, A.E.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-75494629e804d3900179dd0ad68909e0d54cc285de0859f18e7b0e36473912713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Ascitic Fluid - cytology</topic><topic>Ascitic Fluid - drug effects</topic><topic>Ascitic Fluid - metabolism</topic><topic>Benzopsoralen</topic><topic>Biological and medical sciences</topic><topic>Chemistry, Pharmaceutical</topic><topic>Dermatologic Agents - chemistry</topic><topic>Dermatologic Agents - metabolism</topic><topic>Dermatologic Agents - pharmacology</topic><topic>Dermatologic Agents - radiation effects</topic><topic>Drug Carriers</topic><topic>Drug Compounding</topic><topic>Endocytosis</topic><topic>Exudates peritoneal cell</topic><topic>Furocoumarins - chemistry</topic><topic>Furocoumarins - metabolism</topic><topic>Furocoumarins - pharmacology</topic><topic>Furocoumarins - radiation effects</topic><topic>General pharmacology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Lactic Acid - chemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Nanoparticle</topic><topic>Nanoparticles</topic><topic>Particle Size</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Photosensitizing Agents - chemistry</topic><topic>Photosensitizing Agents - metabolism</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Photosensitizing Agents - radiation effects</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Polymers - chemistry</topic><topic>PUVA</topic><topic>PUVA Therapy</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Solubility</topic><topic>Surface Properties</topic><topic>Technology, Pharmaceutical - methods</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gomes, A.J.</creatorcontrib><creatorcontrib>Faustino, A.S.</creatorcontrib><creatorcontrib>Lunardi, C.N.</creatorcontrib><creatorcontrib>Lunardi, L.O.</creatorcontrib><creatorcontrib>Machado, A.E.H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gomes, A.J.</au><au>Faustino, A.S.</au><au>Lunardi, C.N.</au><au>Lunardi, L.O.</au><au>Machado, A.E.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of nanoparticles loaded with benzopsoralen in rat peritoneal exudate cells</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2007-03-06</date><risdate>2007</risdate><volume>332</volume><issue>1</issue><spage>153</spage><epage>160</epage><pages>153-160</pages><issn>0378-5173</issn><eissn>1873-3476</eissn><coden>IJPHDE</coden><abstract>Psoralens are widely used for the treatment of hyperproliferative skin disease. In this work, we prepared nanoparticles (NP) containing a benzopsoralen (3-ethoxy carbonyl-2
H-benzofuro[3,2-
f]-1-benzopiran-2-one) by the solvent evaporation technique. We evaluated important NP parameters such as particle size, drug encapsulation efficiency, effect of the encapsulation process over the drug's photochemistry, zeta potential, external morphology, and
in vitro release behavior. We also investigated the nanoparticle as a drug delivery system (DDS), as well as its target delivery to the action site, which is a very important parameter to increase the therapeutic use of psoralens and to reduce their side effects. The uptake of benzopsoralen-loaded PLGA nanoparticles by different kinds of cells found in rat peritoneal exudates was also studied. The photodamage promoted by irradiation with UV light revealed morphological characteristics of cell damage such as cytoplasmic vesiculation, mitochondrial damage, and swelling of both the granular endoplasmatic reticulum and nuclear membrane. This encapsulation method maintained the drug's properties and improved drug delivery to the target cell.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17056212</pmid><doi>10.1016/j.ijpharm.2006.09.035</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Ascitic Fluid - cytology Ascitic Fluid - drug effects Ascitic Fluid - metabolism Benzopsoralen Biological and medical sciences Chemistry, Pharmaceutical Dermatologic Agents - chemistry Dermatologic Agents - metabolism Dermatologic Agents - pharmacology Dermatologic Agents - radiation effects Drug Carriers Drug Compounding Endocytosis Exudates peritoneal cell Furocoumarins - chemistry Furocoumarins - metabolism Furocoumarins - pharmacology Furocoumarins - radiation effects General pharmacology In Vitro Techniques Kinetics Lactic Acid - chemistry Male Medical sciences Nanoparticle Nanoparticles Particle Size Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Photosensitizing Agents - chemistry Photosensitizing Agents - metabolism Photosensitizing Agents - pharmacology Photosensitizing Agents - radiation effects Polyglycolic Acid - chemistry Polymers - chemistry PUVA PUVA Therapy Rats Rats, Wistar Solubility Surface Properties Technology, Pharmaceutical - methods Ultraviolet Rays |
| title | Evaluation of nanoparticles loaded with benzopsoralen in rat peritoneal exudate cells |
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