Comparative evaluation of in-house manual, and commercial semi-automated and automated DNA extraction platforms in the sample preparation of human stool specimens for a Salmonella enterica 5'-nuclease assay

In the present study, three methods (NucliSens miniMAG [bioMérieux], MagNA Pure DNA Isolation Kit III Bacteria/Fungi [Roche], and a silica-guanidiniumthiocyanate {Si-GuSCN-F} procedure for extracting DNA from stool specimens were compared with regard to analytical performance (relative DNA recovery...

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Veröffentlicht in:	Journal of microbiological methods 2007-12, Vol.71 (3), p.238-245
Hauptverfasser:	Schuurman, Tim, de Boer, Richard, Patty, Rachèl, Kooistra-Smid, Mirjam, van Zwet, Anton
Format:	Artikel
Sprache:	eng
Schlagworte:	(Semi-)automated Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Biological Assay Diverse techniques DNA extraction DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Endonucleases Evaluation Feces Feces - microbiology Fundamental and applied biological sciences. Psychology Humans Manual Microbiology Molecular and cellular biology Polymerase Chain Reaction Reagent Kits, Diagnostic - economics Real-time PCR Reproducibility of Results Salmonella enterica Salmonella enterica - genetics Salmonella enterica - isolation & purification Sensitivity and Specificity
Online-Zugang:	Volltext
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