Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment
DNA microarray based transcriptome analysis has become widely used in biomedical research; however, the lack of DNA sequence information available for Chinese hamster ovary (CHO) cells has hampered the application of microarrays for this cell line widely used for recombinant therapeutic protein prod...
Gespeichert in:
| Veröffentlicht in: | Journal of bioscience and bioengineering 2007-01, Vol.103 (1), p.82-91 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 91 |
|---|---|
| container_issue | 1 |
| container_start_page | 82 |
| container_title | Journal of bioscience and bioengineering |
| container_volume | 103 |
| creator | De Leon Gatti, Marcela Wlaschin, Katie F. Nissom, Peter Morin Yap, Miranda Hu, Wei-Shou |
| description | DNA microarray based transcriptome analysis has become widely used in biomedical research; however, the lack of DNA sequence information available for Chinese hamster ovary (CHO) cells has hampered the application of microarrays for this cell line widely used for recombinant therapeutic protein production. We have constructed an expressed sequence tag (EST) based CHO DNA microarray and employed it for comparative transcriptome analysis of CHO cells and mouse hybridoma cells treated with sodium butyrate. Cross-species hybridization of CHO transcripts to mouse DNA microarrays was also performed to assess the utility of cross-species microarray. The average identity among probe sequences present on both the CHO and mouse microarray was 89.6%. Although cross-species hybridization yielded non-contradicting results when compared with the same-species arrays, decreased sensitivity was observed and resulted in fewer differentially expressed genes being confidently identified. The comparatively small number of genes probed using the CHO microarray and the low number of genes identified as differentially expressed in the cross-species hybridization limited physiological interpretation of the response of CHO cells to sodium butyrate treatment. Nevertheless, when all results are combined, mouse hybridoma and CHO cells can be seen to respond similarly to butyrate treatment, affecting histone modification, chaperones, lipid metabolism, and protein processing. To further develop the utility of microarray technology in cell culture process development, an expansion of current CHO cell sequencing efforts to increase the coverage of genes on available microarrays is warranted. |
| doi_str_mv | 10.1263/jbb.103.82 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69005459</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1389172307700282</els_id><sourcerecordid>3096231271</sourcerecordid><originalsourceid>FETCH-LOGICAL-c556t-e2dc3000229fdae669bf5831f1613a33575934f025f693e438b79e2def2342543</originalsourceid><addsrcrecordid>eNqFkU2LFDEQhhtR3HX14l0JiB6EHvPZnRxl8JMFPei5SXdXZjN0J2OSHpij_9xqZmBBBC9JUfWkqvK-VfWc0Q3jjXi37_sNo2Kj-YPqmgnZ1lJy9nCNtalZy8VV9STnPaWspS17XF1hzmhD1XX1exvng022-COQkmzIQ_KH4mOwE7F4nLLPJDoyxyUDuTv1yY9xtlgbSYIhzr0PNhSyvfMBVsLOuUAi8WjTiQwwTZksYYS0iz7sSL-UE05bZ4EtM4TytHrk7JTh2eW-qX5-_PBj-7m-_fbpy_b9bT0o1ZQa-DgISinnxo0Wmsb0TmnBHGuYsEKoVhkhHeXKNUaAFLpvDT4Cx4XkSoqb6s257yHFXwvk0s0-r_vZAPi3rjGUKolN_gcyg-o17Qq--gvcxyWhZshIyYSgTGuk3p6pIcWcE7jukPyM4nSMdqt_HfqHseg0R_jlpeXSzzDeoxfDEHh9AWwe7OTQscHne04rbpiiyL04c87Gzu4SMl-_c0pbSnXDW6zLcx1Q8qOH1OXBQxhg9Ohq6cbo_7XfH9DDvvk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1441330188</pqid></control><display><type>article</type><title>Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>De Leon Gatti, Marcela ; Wlaschin, Katie F. ; Nissom, Peter Morin ; Yap, Miranda ; Hu, Wei-Shou</creator><creatorcontrib>De Leon Gatti, Marcela ; Wlaschin, Katie F. ; Nissom, Peter Morin ; Yap, Miranda ; Hu, Wei-Shou</creatorcontrib><description>DNA microarray based transcriptome analysis has become widely used in biomedical research; however, the lack of DNA sequence information available for Chinese hamster ovary (CHO) cells has hampered the application of microarrays for this cell line widely used for recombinant therapeutic protein production. We have constructed an expressed sequence tag (EST) based CHO DNA microarray and employed it for comparative transcriptome analysis of CHO cells and mouse hybridoma cells treated with sodium butyrate. Cross-species hybridization of CHO transcripts to mouse DNA microarrays was also performed to assess the utility of cross-species microarray. The average identity among probe sequences present on both the CHO and mouse microarray was 89.6%. Although cross-species hybridization yielded non-contradicting results when compared with the same-species arrays, decreased sensitivity was observed and resulted in fewer differentially expressed genes being confidently identified. The comparatively small number of genes probed using the CHO microarray and the low number of genes identified as differentially expressed in the cross-species hybridization limited physiological interpretation of the response of CHO cells to sodium butyrate treatment. Nevertheless, when all results are combined, mouse hybridoma and CHO cells can be seen to respond similarly to butyrate treatment, affecting histone modification, chaperones, lipid metabolism, and protein processing. To further develop the utility of microarray technology in cell culture process development, an expansion of current CHO cell sequencing efforts to increase the coverage of genes on available microarrays is warranted.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1263/jbb.103.82</identifier><identifier>PMID: 17298905</identifier><identifier>CODEN: JFBIEX</identifier><language>eng</language><publisher>Amsterdarm: Elsevier B.V</publisher><subject>ACIDE BUTYRIQUE ; ACIDO BUTIRICO ; Animals ; ANTIBODIES ; antibody production ; ANTICORPS ; ANTICUERPOS ; Biological and medical sciences ; Biotechnology ; Butyrates - administration & dosage ; BUTYRIC ACID ; cDNA microarray ; CELL CULTURE ; Cell Line ; CHO Cells - metabolism ; Cricetinae ; Cricetulus ; cross-species hybridization ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; EXPRESION GENICA ; expressed sequence tag (EST) ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE EXPRESSION ; Gene Expression Profiling - methods ; GENETICALLY MODIFIED ORGANISMS ; genomics ; HIBRIDOMA ; HYBRIDOMAS ; Hybridomas - drug effects ; Hybridomas - metabolism ; HYBRIDOME ; Mice ; Oligonucleotide Array Sequence Analysis - methods ; ORGANISME GENETIQUEMENT MODIFIE ; ORGANISMOS MODIFICADOS GENETICAMENTE ; Proteome - metabolism ; Recombination, Genetic ; TRANSCRIPCION ; TRANSCRIPTION ; Transcription Factors - metabolism ; Transcription, Genetic - drug effects ; transcriptome</subject><ispartof>Journal of bioscience and bioengineering, 2007-01, Vol.103 (1), p.82-91</ispartof><rights>2007 The Society for Biotechnology, Japan</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-e2dc3000229fdae669bf5831f1613a33575934f025f693e438b79e2def2342543</citedby><cites>FETCH-LOGICAL-c556t-e2dc3000229fdae669bf5831f1613a33575934f025f693e438b79e2def2342543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1389172307700282$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18529150$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17298905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Leon Gatti, Marcela</creatorcontrib><creatorcontrib>Wlaschin, Katie F.</creatorcontrib><creatorcontrib>Nissom, Peter Morin</creatorcontrib><creatorcontrib>Yap, Miranda</creatorcontrib><creatorcontrib>Hu, Wei-Shou</creatorcontrib><title>Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>DNA microarray based transcriptome analysis has become widely used in biomedical research; however, the lack of DNA sequence information available for Chinese hamster ovary (CHO) cells has hampered the application of microarrays for this cell line widely used for recombinant therapeutic protein production. We have constructed an expressed sequence tag (EST) based CHO DNA microarray and employed it for comparative transcriptome analysis of CHO cells and mouse hybridoma cells treated with sodium butyrate. Cross-species hybridization of CHO transcripts to mouse DNA microarrays was also performed to assess the utility of cross-species microarray. The average identity among probe sequences present on both the CHO and mouse microarray was 89.6%. Although cross-species hybridization yielded non-contradicting results when compared with the same-species arrays, decreased sensitivity was observed and resulted in fewer differentially expressed genes being confidently identified. The comparatively small number of genes probed using the CHO microarray and the low number of genes identified as differentially expressed in the cross-species hybridization limited physiological interpretation of the response of CHO cells to sodium butyrate treatment. Nevertheless, when all results are combined, mouse hybridoma and CHO cells can be seen to respond similarly to butyrate treatment, affecting histone modification, chaperones, lipid metabolism, and protein processing. To further develop the utility of microarray technology in cell culture process development, an expansion of current CHO cell sequencing efforts to increase the coverage of genes on available microarrays is warranted.</description><subject>ACIDE BUTYRIQUE</subject><subject>ACIDO BUTIRICO</subject><subject>Animals</subject><subject>ANTIBODIES</subject><subject>antibody production</subject><subject>ANTICORPS</subject><subject>ANTICUERPOS</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Butyrates - administration & dosage</subject><subject>BUTYRIC ACID</subject><subject>cDNA microarray</subject><subject>CELL CULTURE</subject><subject>Cell Line</subject><subject>CHO Cells - metabolism</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>cross-species hybridization</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>EXPRESION GENICA</subject><subject>expressed sequence tag (EST)</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Profiling - methods</subject><subject>GENETICALLY MODIFIED ORGANISMS</subject><subject>genomics</subject><subject>HIBRIDOMA</subject><subject>HYBRIDOMAS</subject><subject>Hybridomas - drug effects</subject><subject>Hybridomas - metabolism</subject><subject>HYBRIDOME</subject><subject>Mice</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>ORGANISME GENETIQUEMENT MODIFIE</subject><subject>ORGANISMOS MODIFICADOS GENETICAMENTE</subject><subject>Proteome - metabolism</subject><subject>Recombination, Genetic</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic - drug effects</subject><subject>transcriptome</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhhtR3HX14l0JiB6EHvPZnRxl8JMFPei5SXdXZjN0J2OSHpij_9xqZmBBBC9JUfWkqvK-VfWc0Q3jjXi37_sNo2Kj-YPqmgnZ1lJy9nCNtalZy8VV9STnPaWspS17XF1hzmhD1XX1exvng022-COQkmzIQ_KH4mOwE7F4nLLPJDoyxyUDuTv1yY9xtlgbSYIhzr0PNhSyvfMBVsLOuUAi8WjTiQwwTZksYYS0iz7sSL-UE05bZ4EtM4TytHrk7JTh2eW-qX5-_PBj-7m-_fbpy_b9bT0o1ZQa-DgISinnxo0Wmsb0TmnBHGuYsEKoVhkhHeXKNUaAFLpvDT4Cx4XkSoqb6s257yHFXwvk0s0-r_vZAPi3rjGUKolN_gcyg-o17Qq--gvcxyWhZshIyYSgTGuk3p6pIcWcE7jukPyM4nSMdqt_HfqHseg0R_jlpeXSzzDeoxfDEHh9AWwe7OTQscHne04rbpiiyL04c87Gzu4SMl-_c0pbSnXDW6zLcx1Q8qOH1OXBQxhg9Ohq6cbo_7XfH9DDvvk</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>De Leon Gatti, Marcela</creator><creator>Wlaschin, Katie F.</creator><creator>Nissom, Peter Morin</creator><creator>Yap, Miranda</creator><creator>Hu, Wei-Shou</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment</title><author>De Leon Gatti, Marcela ; Wlaschin, Katie F. ; Nissom, Peter Morin ; Yap, Miranda ; Hu, Wei-Shou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-e2dc3000229fdae669bf5831f1613a33575934f025f693e438b79e2def2342543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>ACIDE BUTYRIQUE</topic><topic>ACIDO BUTIRICO</topic><topic>Animals</topic><topic>ANTIBODIES</topic><topic>antibody production</topic><topic>ANTICORPS</topic><topic>ANTICUERPOS</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Butyrates - administration & dosage</topic><topic>BUTYRIC ACID</topic><topic>cDNA microarray</topic><topic>CELL CULTURE</topic><topic>Cell Line</topic><topic>CHO Cells - metabolism</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>cross-species hybridization</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>EXPRESION GENICA</topic><topic>expressed sequence tag (EST)</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Profiling - methods</topic><topic>GENETICALLY MODIFIED ORGANISMS</topic><topic>genomics</topic><topic>HIBRIDOMA</topic><topic>HYBRIDOMAS</topic><topic>Hybridomas - drug effects</topic><topic>Hybridomas - metabolism</topic><topic>HYBRIDOME</topic><topic>Mice</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>ORGANISME GENETIQUEMENT MODIFIE</topic><topic>ORGANISMOS MODIFICADOS GENETICAMENTE</topic><topic>Proteome - metabolism</topic><topic>Recombination, Genetic</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic - drug effects</topic><topic>transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Leon Gatti, Marcela</creatorcontrib><creatorcontrib>Wlaschin, Katie F.</creatorcontrib><creatorcontrib>Nissom, Peter Morin</creatorcontrib><creatorcontrib>Yap, Miranda</creatorcontrib><creatorcontrib>Hu, Wei-Shou</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Leon Gatti, Marcela</au><au>Wlaschin, Katie F.</au><au>Nissom, Peter Morin</au><au>Yap, Miranda</au><au>Hu, Wei-Shou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>103</volume><issue>1</issue><spage>82</spage><epage>91</epage><pages>82-91</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><coden>JFBIEX</coden><abstract>DNA microarray based transcriptome analysis has become widely used in biomedical research; however, the lack of DNA sequence information available for Chinese hamster ovary (CHO) cells has hampered the application of microarrays for this cell line widely used for recombinant therapeutic protein production. We have constructed an expressed sequence tag (EST) based CHO DNA microarray and employed it for comparative transcriptome analysis of CHO cells and mouse hybridoma cells treated with sodium butyrate. Cross-species hybridization of CHO transcripts to mouse DNA microarrays was also performed to assess the utility of cross-species microarray. The average identity among probe sequences present on both the CHO and mouse microarray was 89.6%. Although cross-species hybridization yielded non-contradicting results when compared with the same-species arrays, decreased sensitivity was observed and resulted in fewer differentially expressed genes being confidently identified. The comparatively small number of genes probed using the CHO microarray and the low number of genes identified as differentially expressed in the cross-species hybridization limited physiological interpretation of the response of CHO cells to sodium butyrate treatment. Nevertheless, when all results are combined, mouse hybridoma and CHO cells can be seen to respond similarly to butyrate treatment, affecting histone modification, chaperones, lipid metabolism, and protein processing. To further develop the utility of microarray technology in cell culture process development, an expansion of current CHO cell sequencing efforts to increase the coverage of genes on available microarrays is warranted.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><pmid>17298905</pmid><doi>10.1263/jbb.103.82</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 2007-01, Vol.103 (1), p.82-91 |
| issn | 1389-1723 1347-4421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69005459 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ACIDE BUTYRIQUE ACIDO BUTIRICO Animals ANTIBODIES antibody production ANTICORPS ANTICUERPOS Biological and medical sciences Biotechnology Butyrates - administration & dosage BUTYRIC ACID cDNA microarray CELL CULTURE Cell Line CHO Cells - metabolism Cricetinae Cricetulus cross-species hybridization CULTIVO DE CELULAS CULTURE DE CELLULE EXPRESION GENICA expressed sequence tag (EST) EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE EXPRESSION Gene Expression Profiling - methods GENETICALLY MODIFIED ORGANISMS genomics HIBRIDOMA HYBRIDOMAS Hybridomas - drug effects Hybridomas - metabolism HYBRIDOME Mice Oligonucleotide Array Sequence Analysis - methods ORGANISME GENETIQUEMENT MODIFIE ORGANISMOS MODIFICADOS GENETICAMENTE Proteome - metabolism Recombination, Genetic TRANSCRIPCION TRANSCRIPTION Transcription Factors - metabolism Transcription, Genetic - drug effects transcriptome |
| title | Comparative transcriptional analysis of mouse hybridoma and recombinant Chinese hamster ovary cells undergoing butyrate treatment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T19%3A41%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20transcriptional%20analysis%20of%20mouse%20hybridoma%20and%20recombinant%20Chinese%20hamster%20ovary%20cells%20undergoing%20butyrate%20treatment&rft.jtitle=Journal%20of%20bioscience%20and%20bioengineering&rft.au=De%20Leon%20Gatti,%20Marcela&rft.date=2007-01-01&rft.volume=103&rft.issue=1&rft.spage=82&rft.epage=91&rft.pages=82-91&rft.issn=1389-1723&rft.eissn=1347-4421&rft.coden=JFBIEX&rft_id=info:doi/10.1263/jbb.103.82&rft_dat=%3Cproquest_cross%3E3096231271%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1441330188&rft_id=info:pmid/17298905&rft_els_id=S1389172307700282&rfr_iscdi=true |