Immunoassay for acetamiprid detection: application to residue analysis and comparison with liquid chromatography
This work describes the fundamental ability of a commercial ELISA to determine acetamiprid and the application of the ELISA to residue analysis in fruit and vegetable samples. The ELISA exhibited satisfactory sensitivity (I ₅₀ 0.6 ng/g; limit of detection 0.053 ng/g) and a high selectivity for aceta...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2006-11, Vol.386 (5), p.1441-1448 |
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| creator | Watanabe, Eiki Miyake, Shiro Baba, Koji Eun, Heesoo Endo, Shozo |
| description | This work describes the fundamental ability of a commercial ELISA to determine acetamiprid and the application of the ELISA to residue analysis in fruit and vegetable samples. The ELISA exhibited satisfactory sensitivity (I ₅₀ 0.6 ng/g; limit of detection 0.053 ng/g) and a high selectivity for acetamiprid versus other neonicotinoid analogs (thiacloprid amide). Methanol, which influenced the sensitivity of the ELISA the least, was selected as the extractant for the ELISA analysis. Simple dilution of sample extracts with water eliminated matrix interferences. Average recoveries from the acetamiprid-spiked agricultural samples were >95% using a simple extraction method. Analytical results obtained from the ELISA were comparable to those obtained from the reference HPLC method (r>0.99). The ELISA applied to the residue analysis of acetamiprid in agricultural products is a rapid, simple, and cost-effective method, and could be successfully applied to the detection of acetamiprid before the distribution of produce. |
| doi_str_mv | 10.1007/s00216-006-0683-z |
| format | Article |
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The ELISA exhibited satisfactory sensitivity (I ₅₀ 0.6 ng/g; limit of detection 0.053 ng/g) and a high selectivity for acetamiprid versus other neonicotinoid analogs (thiacloprid amide). Methanol, which influenced the sensitivity of the ELISA the least, was selected as the extractant for the ELISA analysis. Simple dilution of sample extracts with water eliminated matrix interferences. Average recoveries from the acetamiprid-spiked agricultural samples were >95% using a simple extraction method. Analytical results obtained from the ELISA were comparable to those obtained from the reference HPLC method (r>0.99). The ELISA applied to the residue analysis of acetamiprid in agricultural products is a rapid, simple, and cost-effective method, and could be successfully applied to the detection of acetamiprid before the distribution of produce.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-006-0683-z</identifier><identifier>PMID: 16917672</identifier><language>eng</language><publisher>Germany: Berlin/Heidelberg : Springer-Verlag</publisher><subject>acetamiprid ; Agricultural products ; Agricultural sample ; Chromatography, High Pressure Liquid - methods ; Dilution ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; High performance liquid chromatography ; Immunoassay ; Liquid chromatography ; Molecular Structure ; Neonicotinoids ; Pesticide residue analysis ; Pyridines - analysis ; Reproducibility of Results ; Residues ; Selectivity ; Sensitivity and Specificity ; Thiacloprid</subject><ispartof>Analytical and bioanalytical chemistry, 2006-11, Vol.386 (5), p.1441-1448</ispartof><rights>Springer-Verlag 2006.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-96b42d680bb78117af7f6fc7e93203739da54bf570f17fa7e069ecb1b22acf583</citedby><cites>FETCH-LOGICAL-c382t-96b42d680bb78117af7f6fc7e93203739da54bf570f17fa7e069ecb1b22acf583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16917672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Eiki</creatorcontrib><creatorcontrib>Miyake, Shiro</creatorcontrib><creatorcontrib>Baba, Koji</creatorcontrib><creatorcontrib>Eun, Heesoo</creatorcontrib><creatorcontrib>Endo, Shozo</creatorcontrib><title>Immunoassay for acetamiprid detection: application to residue analysis and comparison with liquid chromatography</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><description>This work describes the fundamental ability of a commercial ELISA to determine acetamiprid and the application of the ELISA to residue analysis in fruit and vegetable samples. 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The ELISA applied to the residue analysis of acetamiprid in agricultural products is a rapid, simple, and cost-effective method, and could be successfully applied to the detection of acetamiprid before the distribution of produce.</description><subject>acetamiprid</subject><subject>Agricultural products</subject><subject>Agricultural sample</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dilution</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>High performance liquid chromatography</subject><subject>Immunoassay</subject><subject>Liquid chromatography</subject><subject>Molecular Structure</subject><subject>Neonicotinoids</subject><subject>Pesticide residue analysis</subject><subject>Pyridines - analysis</subject><subject>Reproducibility of Results</subject><subject>Residues</subject><subject>Selectivity</subject><subject>Sensitivity and Specificity</subject><subject>Thiacloprid</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1LHTEUhoMo1Wp_QDcaKHQ39iSZJDPuRPohCC6q63Amk3gjMzdjMkO5_npzuRcL3XQRcg485128DyGfGVwyAP0tA3CmKoDyVCOq1wNywhRrKq4kHL7PNT8mH3N-BmCyYeoDOWaqZVppfkKm23Fc1hFzxg31MVG0bsYxTCn0tHezs3OI6yuK0zQEi9uFzpEml0O_OIprHDY55DL01MZxwhRyQf6EeUWH8LKUFLtKccQ5PiWcVpszcuRxyO7T_j8ljz--P9z8qu7uf97eXN9VVjR8rlrV1bxXDXSdbhjT6LVX3mrXCg5Ci7ZHWXdeavBMe9QOVOtsxzrO0XrZiFPydZc7pfiyuDybMWTrhgHXLi7ZqBagrqX8L8hbqbRq6wJ--Qd8jksqBRRGKSm0BIBCsR1lU8w5OW9KlSOmjWFgttbMzpop1szWmnktN-f75KUbXf_3Yq-pABc7wGM0-FQ6No-_OTBR8nSR3Yg3m9WeBg</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Watanabe, Eiki</creator><creator>Miyake, Shiro</creator><creator>Baba, Koji</creator><creator>Eun, Heesoo</creator><creator>Endo, Shozo</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20061101</creationdate><title>Immunoassay for acetamiprid detection: application to residue analysis and comparison with liquid chromatography</title><author>Watanabe, Eiki ; Miyake, Shiro ; Baba, Koji ; Eun, Heesoo ; Endo, Shozo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-96b42d680bb78117af7f6fc7e93203739da54bf570f17fa7e069ecb1b22acf583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>acetamiprid</topic><topic>Agricultural products</topic><topic>Agricultural sample</topic><topic>Chromatography, High Pressure Liquid - 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Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Eiki</au><au>Miyake, Shiro</au><au>Baba, Koji</au><au>Eun, Heesoo</au><au>Endo, Shozo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunoassay for acetamiprid detection: application to residue analysis and comparison with liquid chromatography</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><addtitle>Anal Bioanal Chem</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>386</volume><issue>5</issue><spage>1441</spage><epage>1448</epage><pages>1441-1448</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>This work describes the fundamental ability of a commercial ELISA to determine acetamiprid and the application of the ELISA to residue analysis in fruit and vegetable samples. The ELISA exhibited satisfactory sensitivity (I ₅₀ 0.6 ng/g; limit of detection 0.053 ng/g) and a high selectivity for acetamiprid versus other neonicotinoid analogs (thiacloprid amide). Methanol, which influenced the sensitivity of the ELISA the least, was selected as the extractant for the ELISA analysis. Simple dilution of sample extracts with water eliminated matrix interferences. Average recoveries from the acetamiprid-spiked agricultural samples were >95% using a simple extraction method. Analytical results obtained from the ELISA were comparable to those obtained from the reference HPLC method (r>0.99). The ELISA applied to the residue analysis of acetamiprid in agricultural products is a rapid, simple, and cost-effective method, and could be successfully applied to the detection of acetamiprid before the distribution of produce.</abstract><cop>Germany</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>16917672</pmid><doi>10.1007/s00216-006-0683-z</doi><tpages>8</tpages></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2006-11, Vol.386 (5), p.1441-1448 |
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| subjects | acetamiprid Agricultural products Agricultural sample Chromatography, High Pressure Liquid - methods Dilution Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods High performance liquid chromatography Immunoassay Liquid chromatography Molecular Structure Neonicotinoids Pesticide residue analysis Pyridines - analysis Reproducibility of Results Residues Selectivity Sensitivity and Specificity Thiacloprid |
| title | Immunoassay for acetamiprid detection: application to residue analysis and comparison with liquid chromatography |
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