Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites

A loop-mediated isothermal amplification (LAMP) technique has been used as a novel nucleic acid detection method, whereby the target DNA can be amplified with high specificity and sensitivity under an isothermal condition using a set of four specific primers. In this study, we designed two sets of t...

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Veröffentlicht in:	Journal of microbiological methods 2007-12, Vol.71 (3), p.281-287
Hauptverfasser:	Iseki, Hiroshi, Alhassan, Andy, Ohta, Naomi, Thekisoe, Oriel M.M., Yokoyama, Naoaki, Inoue, Noboru, Nambota, Andrew, Yasuda, Jun, Igarashi, Ikuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Babesia - genetics Babesia - isolation & purification Babesia bigemina Babesia bovis Babesia bovis - genetics Babesia bovis - isolation & purification Babesiosis - blood Babesiosis - diagnosis Babesiosis - parasitology Babesiosis - veterinary Biological and medical sciences Cattle Cattle Diseases - blood Cattle Diseases - parasitology DNA Primers - chemistry DNA, Protozoan - analysis DNA, Protozoan - chemistry Fundamental and applied biological sciences. Psychology General aspects and techniques LAMP Multiplex Nucleic Acid Amplification Techniques - methods Nucleic Acid Amplification Techniques - standards Nucleic Acid Amplification Techniques - veterinary Protozoa Restriction enzyme
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