The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis
The actin-nucleating ARP2-ARP3 complex controls cell shape in plants in many different cell types. Its activity is controlled by a multimeric complex containing BRK1 (also known as HSPC300), NAP1, SRA1, ABI and SCAR/WAVE. In this study, we focus on the function of the five putative SCAR homologues i...
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| Veröffentlicht in: | Development (Cambridge) 2007-03, Vol.134 (5), p.967-977 |
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| creator | Uhrig, Joachim F Mutondo, Moola Zimmermann, Ilona Deeks, Michael J Machesky, Laura M Thomas, Philipp Uhrig, Silke Rambke, Claudia Hussey, Patrick J Hülskamp, Martin |
| description | The actin-nucleating ARP2-ARP3 complex controls cell shape in plants in many different cell types. Its activity is controlled by a multimeric complex containing BRK1 (also known as HSPC300), NAP1, SRA1, ABI and SCAR/WAVE. In this study, we focus on the function of the five putative SCAR homologues in Arabidopsis and we provide biochemical evidence that AtSCAR2 can activate the ARP2-ARP3 complex in vitro. Among the single mutants, mutations in only AtSCAR2 result in a subtle or weak phenotype similar to ARP2, ARP3 and other `distorted' mutants. Double-mutant analysis revealed a redundancy with AtSCAR4 . Systematic application of the yeast two-hybrid system and Bimolecular Fluorescence Complementation (BiFC) revealed a complex protein-interaction network between the ARP2-ARP3 complex and its genetically defined regulators. In addition to protein interactions known in other systems, we identified several new interactions, suggesting that SPIKE1 may be an integral component of the SCAR/WAVE complex and that SCAR proteins in plants might act as direct effectors of ROP GTPases. |
| doi_str_mv | 10.1242/dev.02792 |
| format | Article |
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Its activity is controlled by a multimeric complex containing BRK1 (also known as HSPC300), NAP1, SRA1, ABI and SCAR/WAVE. In this study, we focus on the function of the five putative SCAR homologues in Arabidopsis and we provide biochemical evidence that AtSCAR2 can activate the ARP2-ARP3 complex in vitro. Among the single mutants, mutations in only AtSCAR2 result in a subtle or weak phenotype similar to ARP2, ARP3 and other `distorted' mutants. Double-mutant analysis revealed a redundancy with AtSCAR4 . Systematic application of the yeast two-hybrid system and Bimolecular Fluorescence Complementation (BiFC) revealed a complex protein-interaction network between the ARP2-ARP3 complex and its genetically defined regulators. In addition to protein interactions known in other systems, we identified several new interactions, suggesting that SPIKE1 may be an integral component of the SCAR/WAVE complex and that SCAR proteins in plants might act as direct effectors of ROP GTPases.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.02792</identifier><identifier>PMID: 17267444</identifier><language>eng</language><publisher>England: The Company of Biologists Limited</publisher><subject>Actin-Related Protein 2-3 Complex - genetics ; Actin-Related Protein 2-3 Complex - metabolism ; Adenosine Triphosphatases - metabolism ; Arabidopsis ; Arabidopsis - metabolism ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis Proteins - physiology ; Cell Cycle Proteins - metabolism ; Morphogenesis ; Mutation ; Protein Binding ; Protein Interaction Mapping ; Wiskott-Aldrich Syndrome Protein Family - metabolism ; Wiskott-Aldrich Syndrome Protein Family - physiology</subject><ispartof>Development (Cambridge), 2007-03, Vol.134 (5), p.967-977</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-4b0222a94ade27e1ceb0b77f85680e46c51ae420b6ea46b890b95bc10bbed01f3</citedby><cites>FETCH-LOGICAL-c351t-4b0222a94ade27e1ceb0b77f85680e46c51ae420b6ea46b890b95bc10bbed01f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3678,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17267444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Uhrig, Joachim F</creatorcontrib><creatorcontrib>Mutondo, Moola</creatorcontrib><creatorcontrib>Zimmermann, Ilona</creatorcontrib><creatorcontrib>Deeks, Michael J</creatorcontrib><creatorcontrib>Machesky, Laura M</creatorcontrib><creatorcontrib>Thomas, Philipp</creatorcontrib><creatorcontrib>Uhrig, Silke</creatorcontrib><creatorcontrib>Rambke, Claudia</creatorcontrib><creatorcontrib>Hussey, Patrick J</creatorcontrib><creatorcontrib>Hülskamp, Martin</creatorcontrib><title>The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis</title><title>Development (Cambridge)</title><addtitle>Development</addtitle><description>The actin-nucleating ARP2-ARP3 complex controls cell shape in plants in many different cell types. 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Its activity is controlled by a multimeric complex containing BRK1 (also known as HSPC300), NAP1, SRA1, ABI and SCAR/WAVE. In this study, we focus on the function of the five putative SCAR homologues in Arabidopsis and we provide biochemical evidence that AtSCAR2 can activate the ARP2-ARP3 complex in vitro. Among the single mutants, mutations in only AtSCAR2 result in a subtle or weak phenotype similar to ARP2, ARP3 and other `distorted' mutants. Double-mutant analysis revealed a redundancy with AtSCAR4 . Systematic application of the yeast two-hybrid system and Bimolecular Fluorescence Complementation (BiFC) revealed a complex protein-interaction network between the ARP2-ARP3 complex and its genetically defined regulators. 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| language | eng |
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| subjects | Actin-Related Protein 2-3 Complex - genetics Actin-Related Protein 2-3 Complex - metabolism Adenosine Triphosphatases - metabolism Arabidopsis Arabidopsis - metabolism Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Cell Cycle Proteins - metabolism Morphogenesis Mutation Protein Binding Protein Interaction Mapping Wiskott-Aldrich Syndrome Protein Family - metabolism Wiskott-Aldrich Syndrome Protein Family - physiology |
| title | The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis |
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