Oxidative stress induced by torsion of the spermatic cord in young rats
To evaluate the effects of the oxidative stress in an experimental model of torsion/detorsion of the spermatic cord and the legitimacy of this model for oxidative stress studies. Forty-eight male Wistar rats were randomized in two groups (n=24): G-1 (Sham) and G-2 (Ischemia/Reperfusion). All rats re...
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| Veröffentlicht in: | Acta cirúrgica brasileira 2007-01, Vol.22 (1), p.30-33 |
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| container_title | Acta cirúrgica brasileira |
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| creator | Guimarães, Sergio Botelho Aragão, Alan Arruda Santos, Jefferson Menezes Viana Kimura, Osamu de Sandes Barbosa, Paulo Hudson Uchoa Vasconcelos, Paulo Roberto Leitão de |
| description | To evaluate the effects of the oxidative stress in an experimental model of torsion/detorsion of the spermatic cord and the legitimacy of this model for oxidative stress studies.
Forty-eight male Wistar rats were randomized in two groups (n=24): G-1 (Sham) and G-2 (Ischemia/Reperfusion). All rats received intraperitoneal saline injections (2.0 ml), at 21, 9, and 1 h before right spermatic cord torsion or first sham operation. Detorsion or second sham operation was carried out 3 h later followed by testis and blood samples collection (T-0). Additional samples were collected at 1-3-6 h time-points for assessment of testis malonaldehyde, glutathione, and plasma total antioxidant power (TAP).
Spermatic cord torsion/detorsion induced a significant increase in testicular malonaldehyde contents and a significant decrease in glutathione concentrations in ischemic rats compared with sham animals. Additional increase in malonaldehyde levels occurred during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study.
Torsion/detorsion of the spermatic cord for 3 h induces significant lipid peroxidation and reduction in glutathione content of the testis and is, therefore, a valid model for studying the oxidative stress effects of the ischemia/reperfusion injury in young rat testis. |
| doi_str_mv | 10.1590/s0102-86502007000100005 |
| format | Article |
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Forty-eight male Wistar rats were randomized in two groups (n=24): G-1 (Sham) and G-2 (Ischemia/Reperfusion). All rats received intraperitoneal saline injections (2.0 ml), at 21, 9, and 1 h before right spermatic cord torsion or first sham operation. Detorsion or second sham operation was carried out 3 h later followed by testis and blood samples collection (T-0). Additional samples were collected at 1-3-6 h time-points for assessment of testis malonaldehyde, glutathione, and plasma total antioxidant power (TAP).
Spermatic cord torsion/detorsion induced a significant increase in testicular malonaldehyde contents and a significant decrease in glutathione concentrations in ischemic rats compared with sham animals. Additional increase in malonaldehyde levels occurred during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study.
Torsion/detorsion of the spermatic cord for 3 h induces significant lipid peroxidation and reduction in glutathione content of the testis and is, therefore, a valid model for studying the oxidative stress effects of the ischemia/reperfusion injury in young rat testis.</description><identifier>ISSN: 0102-8650</identifier><identifier>EISSN: 0102-8650</identifier><identifier>DOI: 10.1590/s0102-86502007000100005</identifier><identifier>PMID: 17293946</identifier><language>eng</language><publisher>Brazil</publisher><subject>Animals ; Antioxidants - analysis ; Glutathione - blood ; Injections, Intraperitoneal ; Lipid Peroxidation - physiology ; Male ; Malondialdehyde - blood ; Models, Animal ; Oxidative Stress - physiology ; Random Allocation ; Rats ; Rats, Wistar ; Reperfusion Injury - complications ; Sodium Chloride - administration & dosage ; Spermatic Cord Torsion - complications ; Spermatic Cord Torsion - metabolism ; Thiobarbituric Acid Reactive Substances - analysis ; Time Factors</subject><ispartof>Acta cirúrgica brasileira, 2007-01, Vol.22 (1), p.30-33</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-44a17389e485d304a3d1f324fb80e660be8c1234820f4acf264f07d9587736aa3</citedby><cites>FETCH-LOGICAL-c465t-44a17389e485d304a3d1f324fb80e660be8c1234820f4acf264f07d9587736aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17293946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guimarães, Sergio Botelho</creatorcontrib><creatorcontrib>Aragão, Alan Arruda</creatorcontrib><creatorcontrib>Santos, Jefferson Menezes Viana</creatorcontrib><creatorcontrib>Kimura, Osamu de Sandes</creatorcontrib><creatorcontrib>Barbosa, Paulo Hudson Uchoa</creatorcontrib><creatorcontrib>Vasconcelos, Paulo Roberto Leitão de</creatorcontrib><title>Oxidative stress induced by torsion of the spermatic cord in young rats</title><title>Acta cirúrgica brasileira</title><addtitle>Acta Cir Bras</addtitle><description>To evaluate the effects of the oxidative stress in an experimental model of torsion/detorsion of the spermatic cord and the legitimacy of this model for oxidative stress studies.
Forty-eight male Wistar rats were randomized in two groups (n=24): G-1 (Sham) and G-2 (Ischemia/Reperfusion). All rats received intraperitoneal saline injections (2.0 ml), at 21, 9, and 1 h before right spermatic cord torsion or first sham operation. Detorsion or second sham operation was carried out 3 h later followed by testis and blood samples collection (T-0). Additional samples were collected at 1-3-6 h time-points for assessment of testis malonaldehyde, glutathione, and plasma total antioxidant power (TAP).
Spermatic cord torsion/detorsion induced a significant increase in testicular malonaldehyde contents and a significant decrease in glutathione concentrations in ischemic rats compared with sham animals. Additional increase in malonaldehyde levels occurred during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study.
Torsion/detorsion of the spermatic cord for 3 h induces significant lipid peroxidation and reduction in glutathione content of the testis and is, therefore, a valid model for studying the oxidative stress effects of the ischemia/reperfusion injury in young rat testis.</description><subject>Animals</subject><subject>Antioxidants - analysis</subject><subject>Glutathione - blood</subject><subject>Injections, Intraperitoneal</subject><subject>Lipid Peroxidation - physiology</subject><subject>Male</subject><subject>Malondialdehyde - blood</subject><subject>Models, Animal</subject><subject>Oxidative Stress - physiology</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reperfusion Injury - complications</subject><subject>Sodium Chloride - administration & dosage</subject><subject>Spermatic Cord Torsion - complications</subject><subject>Spermatic Cord Torsion - metabolism</subject><subject>Thiobarbituric Acid Reactive Substances - analysis</subject><subject>Time Factors</subject><issn>0102-8650</issn><issn>0102-8650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkEtPwzAMgCMEYmPwFyAnbgWneTQ9ogkGEtIOwLlK84CitRlJiti_J9MmQMIX2_JnW_oQuiBwRXgN1xEIlIUUHEqACiC3OfgBmv4MDv_UE3QS43ummCD0GE1IVda0ZmKKFsuvzqjUfVocU7Ax4m4wo7YGtxucfIidH7B3OL1lYG1Dn1mNtQ8mg3jjx-EVB5XiKTpyahXt2T7P0Mvd7fP8vnhcLh7mN4-FZoKngjFFKipryyQ3FJiihjhaMtdKsEJAa6UmJWWyBMeUdqVgDipTc1lVVChFZ-hyd3cd_MdoY2r6Lmq7WqnB-jE2QtY14ZxnsNqBOvgYg3XNOnS9CpuGQLN12Dxt9TT_HObN8_2Lse2t-d3bS6PflAZroA</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Guimarães, Sergio Botelho</creator><creator>Aragão, Alan Arruda</creator><creator>Santos, Jefferson Menezes Viana</creator><creator>Kimura, Osamu de Sandes</creator><creator>Barbosa, Paulo Hudson Uchoa</creator><creator>Vasconcelos, Paulo Roberto Leitão de</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Oxidative stress induced by torsion of the spermatic cord in young rats</title><author>Guimarães, Sergio Botelho ; Aragão, Alan Arruda ; Santos, Jefferson Menezes Viana ; Kimura, Osamu de Sandes ; Barbosa, Paulo Hudson Uchoa ; Vasconcelos, Paulo Roberto Leitão de</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-44a17389e485d304a3d1f324fb80e660be8c1234820f4acf264f07d9587736aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antioxidants - analysis</topic><topic>Glutathione - blood</topic><topic>Injections, Intraperitoneal</topic><topic>Lipid Peroxidation - physiology</topic><topic>Male</topic><topic>Malondialdehyde - blood</topic><topic>Models, Animal</topic><topic>Oxidative Stress - physiology</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reperfusion Injury - complications</topic><topic>Sodium Chloride - administration & dosage</topic><topic>Spermatic Cord Torsion - complications</topic><topic>Spermatic Cord Torsion - metabolism</topic><topic>Thiobarbituric Acid Reactive Substances - analysis</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guimarães, Sergio Botelho</creatorcontrib><creatorcontrib>Aragão, Alan Arruda</creatorcontrib><creatorcontrib>Santos, Jefferson Menezes Viana</creatorcontrib><creatorcontrib>Kimura, Osamu de Sandes</creatorcontrib><creatorcontrib>Barbosa, Paulo Hudson Uchoa</creatorcontrib><creatorcontrib>Vasconcelos, Paulo Roberto Leitão de</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta cirúrgica brasileira</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guimarães, Sergio Botelho</au><au>Aragão, Alan Arruda</au><au>Santos, Jefferson Menezes Viana</au><au>Kimura, Osamu de Sandes</au><au>Barbosa, Paulo Hudson Uchoa</au><au>Vasconcelos, Paulo Roberto Leitão de</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxidative stress induced by torsion of the spermatic cord in young rats</atitle><jtitle>Acta cirúrgica brasileira</jtitle><addtitle>Acta Cir Bras</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>22</volume><issue>1</issue><spage>30</spage><epage>33</epage><pages>30-33</pages><issn>0102-8650</issn><eissn>0102-8650</eissn><abstract>To evaluate the effects of the oxidative stress in an experimental model of torsion/detorsion of the spermatic cord and the legitimacy of this model for oxidative stress studies.
Forty-eight male Wistar rats were randomized in two groups (n=24): G-1 (Sham) and G-2 (Ischemia/Reperfusion). All rats received intraperitoneal saline injections (2.0 ml), at 21, 9, and 1 h before right spermatic cord torsion or first sham operation. Detorsion or second sham operation was carried out 3 h later followed by testis and blood samples collection (T-0). Additional samples were collected at 1-3-6 h time-points for assessment of testis malonaldehyde, glutathione, and plasma total antioxidant power (TAP).
Spermatic cord torsion/detorsion induced a significant increase in testicular malonaldehyde contents and a significant decrease in glutathione concentrations in ischemic rats compared with sham animals. Additional increase in malonaldehyde levels occurred during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study.
Torsion/detorsion of the spermatic cord for 3 h induces significant lipid peroxidation and reduction in glutathione content of the testis and is, therefore, a valid model for studying the oxidative stress effects of the ischemia/reperfusion injury in young rat testis.</abstract><cop>Brazil</cop><pmid>17293946</pmid><doi>10.1590/s0102-86502007000100005</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antioxidants - analysis Glutathione - blood Injections, Intraperitoneal Lipid Peroxidation - physiology Male Malondialdehyde - blood Models, Animal Oxidative Stress - physiology Random Allocation Rats Rats, Wistar Reperfusion Injury - complications Sodium Chloride - administration & dosage Spermatic Cord Torsion - complications Spermatic Cord Torsion - metabolism Thiobarbituric Acid Reactive Substances - analysis Time Factors |
| title | Oxidative stress induced by torsion of the spermatic cord in young rats |
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