Sample prevalence and molecular characterisation of Cryptosporidium andersoni within a dairy herd in the United Kingdom
Bovine cryptosporidiosis is usually an acute diarrhoeal disease of young calves caused by Cryptosporidium parvum. However, chronic infection with Cryptosporidium andersoni has been associated with gastritis, reduced milk yield and poor weight gain in adult cattle. Here we describe the first genetic...
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| Veröffentlicht in: | Veterinary parasitology 2006-11, Vol.142 (1), p.163-167 |
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| creator | Robinson, G. Thomas, A.L. Daniel, R.G. Hadfield, S.J. Elwin, K. Chalmers, R.M. |
| description | Bovine cryptosporidiosis is usually an acute diarrhoeal disease of young calves caused by
Cryptosporidium parvum. However, chronic infection with
Cryptosporidium andersoni has been associated with gastritis, reduced milk yield and poor weight gain in adult cattle. Here we describe the first genetic confirmation and characterisation of
C. andersoni from cattle in the United Kingdom and its sample prevalence within a dairy herd. Oocysts measured 7.5
±
0.4
μm
×
5.5
±
0.4
μm (7.0–8.5
μm
×
4.5–6.5
μm) with a length-to-width ratio of 1.37 (1.08–1.60). The within-herd sample prevalence was 16% (95% confidence intervals
=
10.4–21.6%). Nested polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and sequence analysis of the small subunit rDNA was used to confirm the species and characterise the isolates. Due to the lack of overt, acute, clinical symptoms, the incidence, prevalence and importance of this parasite is probably currently underestimated in cattle in the UK. The potential for zoonotic transmission is unknown. |
| doi_str_mv | 10.1016/j.vetpar.2006.06.031 |
| format | Article |
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Cryptosporidium parvum. However, chronic infection with
Cryptosporidium andersoni has been associated with gastritis, reduced milk yield and poor weight gain in adult cattle. Here we describe the first genetic confirmation and characterisation of
C. andersoni from cattle in the United Kingdom and its sample prevalence within a dairy herd. Oocysts measured 7.5
±
0.4
μm
×
5.5
±
0.4
μm (7.0–8.5
μm
×
4.5–6.5
μm) with a length-to-width ratio of 1.37 (1.08–1.60). The within-herd sample prevalence was 16% (95% confidence intervals
=
10.4–21.6%). Nested polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and sequence analysis of the small subunit rDNA was used to confirm the species and characterise the isolates. Due to the lack of overt, acute, clinical symptoms, the incidence, prevalence and importance of this parasite is probably currently underestimated in cattle in the UK. The potential for zoonotic transmission is unknown.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2006.06.031</identifier><identifier>PMID: 16908101</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; bovine cryptosporidiosis ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - parasitology ; cryptosporidiosis ; Cryptosporidiosis - epidemiology ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - veterinary ; Cryptosporidium ; Cryptosporidium - classification ; Cryptosporidium - immunology ; Cryptosporidium - isolation & purification ; Cryptosporidium andersoni ; dairy cattle ; dairy herds ; disease prevalence ; DNA, Ribosomal - analysis ; feces ; Feces - parasitology ; Female ; Herd prevalence ; Molecular characterisation ; molecular systematics ; oocysts ; pathogen identification ; PCR-RFLP ; Phylogeny ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; Prevalence ; restriction fragment length polymorphism ; ribosomal DNA ; sampling ; Sequence analysis ; Wales - epidemiology</subject><ispartof>Veterinary parasitology, 2006-11, Vol.142 (1), p.163-167</ispartof><rights>2006 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-b3b269e7936ee80514ec31d4849617b2a490628221fee1d39419404f21db14a43</citedby><cites>FETCH-LOGICAL-c384t-b3b269e7936ee80514ec31d4849617b2a490628221fee1d39419404f21db14a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2006.06.031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16908101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robinson, G.</creatorcontrib><creatorcontrib>Thomas, A.L.</creatorcontrib><creatorcontrib>Daniel, R.G.</creatorcontrib><creatorcontrib>Hadfield, S.J.</creatorcontrib><creatorcontrib>Elwin, K.</creatorcontrib><creatorcontrib>Chalmers, R.M.</creatorcontrib><title>Sample prevalence and molecular characterisation of Cryptosporidium andersoni within a dairy herd in the United Kingdom</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>Bovine cryptosporidiosis is usually an acute diarrhoeal disease of young calves caused by
Cryptosporidium parvum. However, chronic infection with
Cryptosporidium andersoni has been associated with gastritis, reduced milk yield and poor weight gain in adult cattle. Here we describe the first genetic confirmation and characterisation of
C. andersoni from cattle in the United Kingdom and its sample prevalence within a dairy herd. Oocysts measured 7.5
±
0.4
μm
×
5.5
±
0.4
μm (7.0–8.5
μm
×
4.5–6.5
μm) with a length-to-width ratio of 1.37 (1.08–1.60). The within-herd sample prevalence was 16% (95% confidence intervals
=
10.4–21.6%). Nested polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and sequence analysis of the small subunit rDNA was used to confirm the species and characterise the isolates. Due to the lack of overt, acute, clinical symptoms, the incidence, prevalence and importance of this parasite is probably currently underestimated in cattle in the UK. The potential for zoonotic transmission is unknown.</description><subject>Animals</subject><subject>bovine cryptosporidiosis</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - parasitology</subject><subject>cryptosporidiosis</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - immunology</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium andersoni</subject><subject>dairy cattle</subject><subject>dairy herds</subject><subject>disease prevalence</subject><subject>DNA, Ribosomal - analysis</subject><subject>feces</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Herd prevalence</subject><subject>Molecular characterisation</subject><subject>molecular systematics</subject><subject>oocysts</subject><subject>pathogen identification</subject><subject>PCR-RFLP</subject><subject>Phylogeny</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Prevalence</subject><subject>restriction fragment length polymorphism</subject><subject>ribosomal DNA</subject><subject>sampling</subject><subject>Sequence analysis</subject><subject>Wales - epidemiology</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV2L1DAUhoMo7uzqPxDNlXcd8zVpeiPIoK644MU61yFNTncytE1N0lnm35vSAe88HAghz3k5PEHoHSVbSqj8dNqeIU8mbhkhcrs0py_QhqqaV2y3Iy_RhnAiKkFofYNuUzoRQgSR9Wt0Q2VDVEnZoOdHM0w94CnC2fQwWsBmdHgIPdi5NxHbo4nGZog-mezDiEOH9_Ey5ZCmEL3z87BMQExh9PjZ56MfscHO-HjBR4gOl3s-Aj6MPoPDP_345MLwBr3qTJ_g7fW8Q4dvX3_v76uHX99_7L88VJYrkauWt0w2UDdcAiiyowIsp04o0Uhat8yIhkimGKMdAHW8EbQRRHSMupYKI_gd-rjmTjH8mSFlPfhkoe_NCGFOWqpGyVIFFCtoY0gpQqen6AcTL5oSvQjXJ70K14twvTSnZez9NX9uB3D_hq6GC_BhBToTtHkqGvXhkZUHQksJ1RTi80pA8XD2EHWyfvkJ5yPYrF3w_9_hLzKOnfQ</recordid><startdate>20061130</startdate><enddate>20061130</enddate><creator>Robinson, G.</creator><creator>Thomas, A.L.</creator><creator>Daniel, R.G.</creator><creator>Hadfield, S.J.</creator><creator>Elwin, K.</creator><creator>Chalmers, R.M.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20061130</creationdate><title>Sample prevalence and molecular characterisation of Cryptosporidium andersoni within a dairy herd in the United Kingdom</title><author>Robinson, G. ; Thomas, A.L. ; Daniel, R.G. ; Hadfield, S.J. ; Elwin, K. ; Chalmers, R.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-b3b269e7936ee80514ec31d4849617b2a490628221fee1d39419404f21db14a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>bovine cryptosporidiosis</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - parasitology</topic><topic>cryptosporidiosis</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - classification</topic><topic>Cryptosporidium - immunology</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium andersoni</topic><topic>dairy cattle</topic><topic>dairy herds</topic><topic>disease prevalence</topic><topic>DNA, Ribosomal - analysis</topic><topic>feces</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Herd prevalence</topic><topic>Molecular characterisation</topic><topic>molecular systematics</topic><topic>oocysts</topic><topic>pathogen identification</topic><topic>PCR-RFLP</topic><topic>Phylogeny</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Prevalence</topic><topic>restriction fragment length polymorphism</topic><topic>ribosomal DNA</topic><topic>sampling</topic><topic>Sequence analysis</topic><topic>Wales - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robinson, G.</creatorcontrib><creatorcontrib>Thomas, A.L.</creatorcontrib><creatorcontrib>Daniel, R.G.</creatorcontrib><creatorcontrib>Hadfield, S.J.</creatorcontrib><creatorcontrib>Elwin, K.</creatorcontrib><creatorcontrib>Chalmers, R.M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robinson, G.</au><au>Thomas, A.L.</au><au>Daniel, R.G.</au><au>Hadfield, S.J.</au><au>Elwin, K.</au><au>Chalmers, R.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sample prevalence and molecular characterisation of Cryptosporidium andersoni within a dairy herd in the United Kingdom</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2006-11-30</date><risdate>2006</risdate><volume>142</volume><issue>1</issue><spage>163</spage><epage>167</epage><pages>163-167</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>Bovine cryptosporidiosis is usually an acute diarrhoeal disease of young calves caused by
Cryptosporidium parvum. However, chronic infection with
Cryptosporidium andersoni has been associated with gastritis, reduced milk yield and poor weight gain in adult cattle. Here we describe the first genetic confirmation and characterisation of
C. andersoni from cattle in the United Kingdom and its sample prevalence within a dairy herd. Oocysts measured 7.5
±
0.4
μm
×
5.5
±
0.4
μm (7.0–8.5
μm
×
4.5–6.5
μm) with a length-to-width ratio of 1.37 (1.08–1.60). The within-herd sample prevalence was 16% (95% confidence intervals
=
10.4–21.6%). Nested polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and sequence analysis of the small subunit rDNA was used to confirm the species and characterise the isolates. Due to the lack of overt, acute, clinical symptoms, the incidence, prevalence and importance of this parasite is probably currently underestimated in cattle in the UK. The potential for zoonotic transmission is unknown.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16908101</pmid><doi>10.1016/j.vetpar.2006.06.031</doi><tpages>5</tpages></addata></record> |
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| ispartof | Veterinary parasitology, 2006-11, Vol.142 (1), p.163-167 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68986666 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals bovine cryptosporidiosis Cattle Cattle Diseases - epidemiology Cattle Diseases - parasitology cryptosporidiosis Cryptosporidiosis - epidemiology Cryptosporidiosis - parasitology Cryptosporidiosis - veterinary Cryptosporidium Cryptosporidium - classification Cryptosporidium - immunology Cryptosporidium - isolation & purification Cryptosporidium andersoni dairy cattle dairy herds disease prevalence DNA, Ribosomal - analysis feces Feces - parasitology Female Herd prevalence Molecular characterisation molecular systematics oocysts pathogen identification PCR-RFLP Phylogeny polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length Prevalence restriction fragment length polymorphism ribosomal DNA sampling Sequence analysis Wales - epidemiology |
| title | Sample prevalence and molecular characterisation of Cryptosporidium andersoni within a dairy herd in the United Kingdom |
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