The arsenite oxidase genes ( aroAB) in novel chemoautotrophic arsenite oxidizers
Six novel bacterial strains were isolated from the environment which can oxidize arsenite [As(III)] to the less mobile form arsenate [As(V)] coupled to CO 2 fixation under either aerobic or denitrifying conditions. PCR primers were designed to the conserved molybdopterin domain of the large subunit...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2007-03, Vol.354 (3), p.662-667 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Rhine, E.D. Ní Chadhain, S.M. Zylstra, G.J. Young, L.Y. |
| description | Six novel bacterial strains were isolated from the environment which can oxidize arsenite [As(III)] to the less mobile form arsenate [As(V)] coupled to CO
2 fixation under either aerobic or denitrifying conditions. PCR primers were designed to the conserved molybdopterin domain of the large subunit of arsenite oxidase in order to identify the arsenite oxidase genes from these isolates. The amino acid sequences for the arsenite oxidases reported here were 72–74% identical to that of strain NT-26, the only previously reported autotrophic arsenite oxidizer. Indeed the autotrophic arsenite oxidase genes form a distinct phylogenetic group, separated from previously described heterotrophic arsenite oxidase genes, with the exception of the heterotroph
Agrobacterium tumefaciens. The arsenite oxidase primers described here represent a powerful culture-independent tool to assess the diversity of arsenite oxidase genes in environmental bacteria. |
| doi_str_mv | 10.1016/j.bbrc.2007.01.004 |
| format | Article |
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2 fixation under either aerobic or denitrifying conditions. PCR primers were designed to the conserved molybdopterin domain of the large subunit of arsenite oxidase in order to identify the arsenite oxidase genes from these isolates. The amino acid sequences for the arsenite oxidases reported here were 72–74% identical to that of strain NT-26, the only previously reported autotrophic arsenite oxidizer. Indeed the autotrophic arsenite oxidase genes form a distinct phylogenetic group, separated from previously described heterotrophic arsenite oxidase genes, with the exception of the heterotroph
Agrobacterium tumefaciens. 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| ispartof | Biochemical and biophysical research communications, 2007-03, Vol.354 (3), p.662-667 |
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| subjects | Agrobacterium tumefaciens Agrobacterium tumefaciens - classification Agrobacterium tumefaciens - genetics Amino Acid Sequence Arsenite oxidation Arsenites - chemistry Arsenites - metabolism Autotrophic Carbon Monoxide - metabolism Chemoautotrophic Diversity Molecular Sequence Data Oxidation-Reduction Oxidoreductases - chemistry Oxidoreductases - genetics Oxidoreductases - metabolism PCR primers Phylogeny Polymerase Chain Reaction |
| title | The arsenite oxidase genes ( aroAB) in novel chemoautotrophic arsenite oxidizers |
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