The Homingbac baculovirus cloning system: An alternative way to introduce foreign DNA into baculovirus genomes
An in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes. This system is called the “Homingbac system” because it uses homing endonucleases. The Homingbac system was engineered into the baculoviruses AcMNPV, BmNPV, PxMNPV, RoMNPV, HaSNPV an...
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| Veröffentlicht in: | Journal of virological methods 2007-03, Vol.140 (1), p.59-65 |
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| container_title | Journal of virological methods |
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| creator | Lihoradova, Olga A. Ogay, Irina D. Abdukarimov, Abdusattor A. Azimova, Sh. S. Lynn, Dwight E. Slack, Jeffrey M. |
| description | An
in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes. This system is called the “Homingbac system” because it uses homing endonucleases. The Homingbac system was engineered into the baculoviruses
AcMNPV,
BmNPV,
PxMNPV,
RoMNPV,
HaSNPV and
HzSNPV. All Homingbac viruses were designed to retain the polyhedra phenotype so that they could be inoculated
per os to insects. This is the first time a common
in vitro baculovirus cloning system has been made for multiple baculovirus species that include both groups I and II nucleopolyhedroviruses (NPVs). In this study, the Homingbac system was demonstrated by directly cloning a PCR-amplified β-
glucuronidase gene cassette into a parent Homingbac virus. This new collection of groups I and II NPV Homingbac viruses are a significant expansion of
in vitro cloning technology and are new tools for making recombinant baculoviruses. |
| doi_str_mv | 10.1016/j.jviromet.2006.10.016 |
| format | Article |
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in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes. This system is called the “Homingbac system” because it uses homing endonucleases. The Homingbac system was engineered into the baculoviruses
AcMNPV,
BmNPV,
PxMNPV,
RoMNPV,
HaSNPV and
HzSNPV. All Homingbac viruses were designed to retain the polyhedra phenotype so that they could be inoculated
per os to insects. This is the first time a common
in vitro baculovirus cloning system has been made for multiple baculovirus species that include both groups I and II nucleopolyhedroviruses (NPVs). In this study, the Homingbac system was demonstrated by directly cloning a PCR-amplified β-
glucuronidase gene cassette into a parent Homingbac virus. This new collection of groups I and II NPV Homingbac viruses are a significant expansion of
in vitro cloning technology and are new tools for making recombinant baculoviruses.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2006.10.016</identifier><identifier>PMID: 17141883</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Baculoviridae - genetics ; Baculovirus ; Biological and medical sciences ; Bombyx mori NPV ; Cloning system ; Cloning, Molecular - methods ; DNA, Recombinant - genetics ; DNA, Viral - genetics ; Expression vector system ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors ; Genome, Viral ; Glucuronidase - genetics ; Green Fluorescent Proteins - metabolism ; Homing endonuclease ; Microbiology ; Models, Biological ; Nuclear polyhedrosis virus ; Nucleopolyhedrovirus - genetics ; Polymerase Chain Reaction ; Recombination, Genetic ; Techniques used in virology ; Transfection ; Virology</subject><ispartof>Journal of virological methods, 2007-03, Vol.140 (1), p.59-65</ispartof><rights>2006</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-80d70253bbadf64c14a593a2a6924b343fa70ab45131b20371ded313e88af3e13</citedby><cites>FETCH-LOGICAL-c427t-80d70253bbadf64c14a593a2a6924b343fa70ab45131b20371ded313e88af3e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jviromet.2006.10.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18509550$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17141883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lihoradova, Olga A.</creatorcontrib><creatorcontrib>Ogay, Irina D.</creatorcontrib><creatorcontrib>Abdukarimov, Abdusattor A.</creatorcontrib><creatorcontrib>Azimova, Sh. S.</creatorcontrib><creatorcontrib>Lynn, Dwight E.</creatorcontrib><creatorcontrib>Slack, Jeffrey M.</creatorcontrib><title>The Homingbac baculovirus cloning system: An alternative way to introduce foreign DNA into baculovirus genomes</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>An
in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes. This system is called the “Homingbac system” because it uses homing endonucleases. The Homingbac system was engineered into the baculoviruses
AcMNPV,
BmNPV,
PxMNPV,
RoMNPV,
HaSNPV and
HzSNPV. All Homingbac viruses were designed to retain the polyhedra phenotype so that they could be inoculated
per os to insects. This is the first time a common
in vitro baculovirus cloning system has been made for multiple baculovirus species that include both groups I and II nucleopolyhedroviruses (NPVs). In this study, the Homingbac system was demonstrated by directly cloning a PCR-amplified β-
glucuronidase gene cassette into a parent Homingbac virus. This new collection of groups I and II NPV Homingbac viruses are a significant expansion of
in vitro cloning technology and are new tools for making recombinant baculoviruses.</description><subject>Baculoviridae - genetics</subject><subject>Baculovirus</subject><subject>Biological and medical sciences</subject><subject>Bombyx mori NPV</subject><subject>Cloning system</subject><subject>Cloning, Molecular - methods</subject><subject>DNA, Recombinant - genetics</subject><subject>DNA, Viral - genetics</subject><subject>Expression vector system</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors</subject><subject>Genome, Viral</subject><subject>Glucuronidase - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Homing endonuclease</subject><subject>Microbiology</subject><subject>Models, Biological</subject><subject>Nuclear polyhedrosis virus</subject><subject>Nucleopolyhedrovirus - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Recombination, Genetic</subject><subject>Techniques used in virology</subject><subject>Transfection</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9P2zAYhy3EBIXtKyBfxq3Fb-wkzk5UbAMkNC5wthznTecqscF2OvXb46qdECcOlq1Hz_tH_hFyAWwBDKqr9WK9scGPmBYFY1WGi4yPyAxk3cxZI8UxmWVS5TcXp-QsxjVjrKw5PyGnUIMAKfmMuKe_SO_8aN2q1YbmMw0-d54iNYN3GdO4jQnHH3TpqB4SBqeT3SD9p7c0eWpdCr6bDNLeB7QrR3_-We6o_9BshS4vG7-SL70eIn473Ofk-fevp5u7-cPj7f3N8mFuRFGnuWRdzYqSt63u-koYELpsuC501RSi5YL3uma6FSVwaAvGa-iw48BRSt1zBH5OLvd9X4J_nTAmNdpocBi0Qz9FVclGFgXIT0VoalEJ4Fms9qIJPsaAvXoJdtRhq4CpXSRqrf5HonaR7HjGufDiMGFqR-zeyw4ZZOH7QdDR6KEP2hkb3z1ZsqYsWfau9x7mj9tYDCoai85gZwOapDpvP9vlDefcrsQ</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Lihoradova, Olga A.</creator><creator>Ogay, Irina D.</creator><creator>Abdukarimov, Abdusattor A.</creator><creator>Azimova, Sh. S.</creator><creator>Lynn, Dwight E.</creator><creator>Slack, Jeffrey M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>The Homingbac baculovirus cloning system: An alternative way to introduce foreign DNA into baculovirus genomes</title><author>Lihoradova, Olga A. ; Ogay, Irina D. ; Abdukarimov, Abdusattor A. ; Azimova, Sh. S. ; Lynn, Dwight E. ; Slack, Jeffrey M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-80d70253bbadf64c14a593a2a6924b343fa70ab45131b20371ded313e88af3e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Baculoviridae - genetics</topic><topic>Baculovirus</topic><topic>Biological and medical sciences</topic><topic>Bombyx mori NPV</topic><topic>Cloning system</topic><topic>Cloning, Molecular - methods</topic><topic>DNA, Recombinant - genetics</topic><topic>DNA, Viral - genetics</topic><topic>Expression vector system</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Vectors</topic><topic>Genome, Viral</topic><topic>Glucuronidase - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Homing endonuclease</topic><topic>Microbiology</topic><topic>Models, Biological</topic><topic>Nuclear polyhedrosis virus</topic><topic>Nucleopolyhedrovirus - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Recombination, Genetic</topic><topic>Techniques used in virology</topic><topic>Transfection</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lihoradova, Olga A.</creatorcontrib><creatorcontrib>Ogay, Irina D.</creatorcontrib><creatorcontrib>Abdukarimov, Abdusattor A.</creatorcontrib><creatorcontrib>Azimova, Sh. S.</creatorcontrib><creatorcontrib>Lynn, Dwight E.</creatorcontrib><creatorcontrib>Slack, Jeffrey M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lihoradova, Olga A.</au><au>Ogay, Irina D.</au><au>Abdukarimov, Abdusattor A.</au><au>Azimova, Sh. S.</au><au>Lynn, Dwight E.</au><au>Slack, Jeffrey M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Homingbac baculovirus cloning system: An alternative way to introduce foreign DNA into baculovirus genomes</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>140</volume><issue>1</issue><spage>59</spage><epage>65</epage><pages>59-65</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>An
in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes. This system is called the “Homingbac system” because it uses homing endonucleases. The Homingbac system was engineered into the baculoviruses
AcMNPV,
BmNPV,
PxMNPV,
RoMNPV,
HaSNPV and
HzSNPV. All Homingbac viruses were designed to retain the polyhedra phenotype so that they could be inoculated
per os to insects. This is the first time a common
in vitro baculovirus cloning system has been made for multiple baculovirus species that include both groups I and II nucleopolyhedroviruses (NPVs). In this study, the Homingbac system was demonstrated by directly cloning a PCR-amplified β-
glucuronidase gene cassette into a parent Homingbac virus. This new collection of groups I and II NPV Homingbac viruses are a significant expansion of
in vitro cloning technology and are new tools for making recombinant baculoviruses.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>17141883</pmid><doi>10.1016/j.jviromet.2006.10.016</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of virological methods, 2007-03, Vol.140 (1), p.59-65 |
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| subjects | Baculoviridae - genetics Baculovirus Biological and medical sciences Bombyx mori NPV Cloning system Cloning, Molecular - methods DNA, Recombinant - genetics DNA, Viral - genetics Expression vector system Fundamental and applied biological sciences. Psychology Genetic Vectors Genome, Viral Glucuronidase - genetics Green Fluorescent Proteins - metabolism Homing endonuclease Microbiology Models, Biological Nuclear polyhedrosis virus Nucleopolyhedrovirus - genetics Polymerase Chain Reaction Recombination, Genetic Techniques used in virology Transfection Virology |
| title | The Homingbac baculovirus cloning system: An alternative way to introduce foreign DNA into baculovirus genomes |
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