Separation of adeno-associated virus type 2 empty particles from genome containing vectors by anion-exchange column chromatography

Adeno-associated virus (AAV) empty capsids typically co-purify with genome containing AAV2 vectors purified by column chromatography. This study describes a method to remove empty capsids from genome containing vector particles by anion exchange chromatography. The separation is based on the slightl...

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Veröffentlicht in:	Journal of virological methods 2007-03, Vol.140 (1), p.183-192
Hauptverfasser:	Qu, Guang, Bahr-Davidson, Jennifer, Prado, Joseph, Tai, Alex, Cataniag, Floro, McDonnell, Jennifer, Zhou, Jingmin, Hauck, Bernd, Luna, Jac, Sommer, Jurg M., Smith, Peter, Zhou, Shangzhen, Colosi, Peter, High, Katherine A., Pierce, Glenn F., Wright, J. Fraser
Format:	Artikel
Sprache:	eng
Schlagworte:	Adeno-associated virus Adeno-associated virus 2 Biological and medical sciences Capsid - metabolism Capsid - ultrastructure Chromatography, Ion Exchange - methods Column chromatography Dependovirus - classification Dependovirus - genetics Dependovirus - isolation & purification Empty capsids Fundamental and applied biological sciences. Psychology Genetic Vectors - isolation & purification Genetic Vectors - ultrastructure Hydrogen-Ion Concentration Microbiology Techniques used in virology Virion - ultrastructure Virology
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