DNA methylation and immunohistochemical analysis of the S100A4 calcium binding protein in human prostate cancer
BACKGROUND The role of DNA methylation in the transcriptional regulation of S100A4 is unknown in human prostate cancer. METHODS Critical CpG sites within intron 1 of S100A4 were sequenced in DNA obtained from prostatic adenocarcinoma, non‐malignant epithelium, and prostate cancer cell lines. S100A4...
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| Veröffentlicht in: | The Prostate 2007-03, Vol.67 (4), p.341-347 |
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| creator | Rehman, Ishtiaq Goodarzi, Amin Cross, Simon S. Leiblich, Aaron Catto, James W.F. Phillips, Joshua T. Hamdy, Freddie C. |
| description | BACKGROUND
The role of DNA methylation in the transcriptional regulation of S100A4 is unknown in human prostate cancer.
METHODS
Critical CpG sites within intron 1 of S100A4 were sequenced in DNA obtained from prostatic adenocarcinoma, non‐malignant epithelium, and prostate cancer cell lines. S100A4 protein expression was assessed by immunohistochemistry and Western blotting.
RESULTS
Methylation was seen in all cases of cancer, non‐malignant epithelium, and in prostate cancer cell lines, but was absent in all cases of blood DNA. S100A4 immunoexpression was absent in all cases of malignant and non‐malignant epithelium, while strong–moderate expression was seen in the stroma and lymphocytes. Western blotting showed absent S100A4 expression in LNCaP and Du145 cells and low levels in PC‐3 cells.
CONCLUSIONS
S100A4 protein is not expressed in benign or malignant prostatic epithelium nor in LNCaP and Du145 cells. The mechanism underlying absent S100A4 expression in prostatic epithelium and cell lines may involve methylation. Prostate 67: 341–347, 2007. © 2007 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/pros.20401 |
| format | Article |
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The role of DNA methylation in the transcriptional regulation of S100A4 is unknown in human prostate cancer.
METHODS
Critical CpG sites within intron 1 of S100A4 were sequenced in DNA obtained from prostatic adenocarcinoma, non‐malignant epithelium, and prostate cancer cell lines. S100A4 protein expression was assessed by immunohistochemistry and Western blotting.
RESULTS
Methylation was seen in all cases of cancer, non‐malignant epithelium, and in prostate cancer cell lines, but was absent in all cases of blood DNA. S100A4 immunoexpression was absent in all cases of malignant and non‐malignant epithelium, while strong–moderate expression was seen in the stroma and lymphocytes. Western blotting showed absent S100A4 expression in LNCaP and Du145 cells and low levels in PC‐3 cells.
CONCLUSIONS
S100A4 protein is not expressed in benign or malignant prostatic epithelium nor in LNCaP and Du145 cells. The mechanism underlying absent S100A4 expression in prostatic epithelium and cell lines may involve methylation. Prostate 67: 341–347, 2007. © 2007 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.20401</identifier><identifier>PMID: 17219414</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - physiopathology ; Base Sequence ; benign prostatic hyperplasia ; Blotting, Western ; Calcium-Binding Proteins - genetics ; Calcium-Binding Proteins - metabolism ; Cell Line, Tumor ; CpG Islands ; CpG sites ; DNA Methylation ; Epithelium - physiology ; Gene Expression Regulation, Neoplastic ; Humans ; Immunohistochemistry ; Male ; malignant progression ; Molecular Sequence Data ; Prostate - physiology ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - physiopathology ; S100 Calcium-Binding Protein A4 ; S100 Proteins - genetics ; S100 Proteins - metabolism</subject><ispartof>The Prostate, 2007-03, Vol.67 (4), p.341-347</ispartof><rights>Copyright © 2007 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4311-7be4a8f6050f2a3b2130aa24b0d077b8453b73fb518e996fae96b55a91f41d993</citedby><cites>FETCH-LOGICAL-c4311-7be4a8f6050f2a3b2130aa24b0d077b8453b73fb518e996fae96b55a91f41d993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.20401$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.20401$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17219414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rehman, Ishtiaq</creatorcontrib><creatorcontrib>Goodarzi, Amin</creatorcontrib><creatorcontrib>Cross, Simon S.</creatorcontrib><creatorcontrib>Leiblich, Aaron</creatorcontrib><creatorcontrib>Catto, James W.F.</creatorcontrib><creatorcontrib>Phillips, Joshua T.</creatorcontrib><creatorcontrib>Hamdy, Freddie C.</creatorcontrib><title>DNA methylation and immunohistochemical analysis of the S100A4 calcium binding protein in human prostate cancer</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>BACKGROUND
The role of DNA methylation in the transcriptional regulation of S100A4 is unknown in human prostate cancer.
METHODS
Critical CpG sites within intron 1 of S100A4 were sequenced in DNA obtained from prostatic adenocarcinoma, non‐malignant epithelium, and prostate cancer cell lines. S100A4 protein expression was assessed by immunohistochemistry and Western blotting.
RESULTS
Methylation was seen in all cases of cancer, non‐malignant epithelium, and in prostate cancer cell lines, but was absent in all cases of blood DNA. S100A4 immunoexpression was absent in all cases of malignant and non‐malignant epithelium, while strong–moderate expression was seen in the stroma and lymphocytes. Western blotting showed absent S100A4 expression in LNCaP and Du145 cells and low levels in PC‐3 cells.
CONCLUSIONS
S100A4 protein is not expressed in benign or malignant prostatic epithelium nor in LNCaP and Du145 cells. The mechanism underlying absent S100A4 expression in prostatic epithelium and cell lines may involve methylation. Prostate 67: 341–347, 2007. © 2007 Wiley‐Liss, Inc.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - physiopathology</subject><subject>Base Sequence</subject><subject>benign prostatic hyperplasia</subject><subject>Blotting, Western</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Cell Line, Tumor</subject><subject>CpG Islands</subject><subject>CpG sites</subject><subject>DNA Methylation</subject><subject>Epithelium - physiology</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>malignant progression</subject><subject>Molecular Sequence Data</subject><subject>Prostate - physiology</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - physiopathology</subject><subject>S100 Calcium-Binding Protein A4</subject><subject>S100 Proteins - genetics</subject><subject>S100 Proteins - metabolism</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhq0K1C6FS38A8okDUsr4I_H6uGpLF6lqgYJ6tOxk0riNnTZOBPvv8bIL3JBGsjR-5vH4JeSEwSkD4B-exiGdcpDADsiCgVYFgCxfkAVwBYVkQh2RVyk9AGQc-CE5YoozLZlckOH8ekUDTt2mt5MfIrWxoT6EOQ6dT9NQdxh8bfvct_0m-USHlk4d0tvsWkmar2o_B-p8bHy8p3mXCX2kubo52LhtpMlOmMlY4_iavGxtn_DN_jwm3z9efDtbF1c3l5_OVldFLQVjhXIo7bKtoISWW-E4E2Atlw4aUMotZSmcEq0r2RK1rlqLunJlaTVrJWu0Fsfk3c6b33-eMU0m-FRj39uIw5xMtdQqV5XB9zuwzoumEVvzNPpgx41hYLbxmu0PzO94M_x2b51dwOYfus8zA2wH_PA9bv6jMp-_3tz-kRa7mZw3_vw7Y8dHUymhSnN3fWnWX5Rac3FnuPgFgvmUug</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Rehman, Ishtiaq</creator><creator>Goodarzi, Amin</creator><creator>Cross, Simon S.</creator><creator>Leiblich, Aaron</creator><creator>Catto, James W.F.</creator><creator>Phillips, Joshua T.</creator><creator>Hamdy, Freddie C.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>DNA methylation and immunohistochemical analysis of the S100A4 calcium binding protein in human prostate cancer</title><author>Rehman, Ishtiaq ; Goodarzi, Amin ; Cross, Simon S. ; Leiblich, Aaron ; Catto, James W.F. ; Phillips, Joshua T. ; Hamdy, Freddie C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4311-7be4a8f6050f2a3b2130aa24b0d077b8453b73fb518e996fae96b55a91f41d993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - physiopathology</topic><topic>Base Sequence</topic><topic>benign prostatic hyperplasia</topic><topic>Blotting, Western</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Cell Line, Tumor</topic><topic>CpG Islands</topic><topic>CpG sites</topic><topic>DNA Methylation</topic><topic>Epithelium - physiology</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>malignant progression</topic><topic>Molecular Sequence Data</topic><topic>Prostate - physiology</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Prostatic Neoplasms - physiopathology</topic><topic>S100 Calcium-Binding Protein A4</topic><topic>S100 Proteins - genetics</topic><topic>S100 Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rehman, Ishtiaq</creatorcontrib><creatorcontrib>Goodarzi, Amin</creatorcontrib><creatorcontrib>Cross, Simon S.</creatorcontrib><creatorcontrib>Leiblich, Aaron</creatorcontrib><creatorcontrib>Catto, James W.F.</creatorcontrib><creatorcontrib>Phillips, Joshua T.</creatorcontrib><creatorcontrib>Hamdy, Freddie C.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rehman, Ishtiaq</au><au>Goodarzi, Amin</au><au>Cross, Simon S.</au><au>Leiblich, Aaron</au><au>Catto, James W.F.</au><au>Phillips, Joshua T.</au><au>Hamdy, Freddie C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA methylation and immunohistochemical analysis of the S100A4 calcium binding protein in human prostate cancer</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>67</volume><issue>4</issue><spage>341</spage><epage>347</epage><pages>341-347</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><abstract>BACKGROUND
The role of DNA methylation in the transcriptional regulation of S100A4 is unknown in human prostate cancer.
METHODS
Critical CpG sites within intron 1 of S100A4 were sequenced in DNA obtained from prostatic adenocarcinoma, non‐malignant epithelium, and prostate cancer cell lines. S100A4 protein expression was assessed by immunohistochemistry and Western blotting.
RESULTS
Methylation was seen in all cases of cancer, non‐malignant epithelium, and in prostate cancer cell lines, but was absent in all cases of blood DNA. S100A4 immunoexpression was absent in all cases of malignant and non‐malignant epithelium, while strong–moderate expression was seen in the stroma and lymphocytes. Western blotting showed absent S100A4 expression in LNCaP and Du145 cells and low levels in PC‐3 cells.
CONCLUSIONS
S100A4 protein is not expressed in benign or malignant prostatic epithelium nor in LNCaP and Du145 cells. The mechanism underlying absent S100A4 expression in prostatic epithelium and cell lines may involve methylation. Prostate 67: 341–347, 2007. © 2007 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17219414</pmid><doi>10.1002/pros.20401</doi><tpages>7</tpages></addata></record> |
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| subjects | Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - physiopathology Base Sequence benign prostatic hyperplasia Blotting, Western Calcium-Binding Proteins - genetics Calcium-Binding Proteins - metabolism Cell Line, Tumor CpG Islands CpG sites DNA Methylation Epithelium - physiology Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Male malignant progression Molecular Sequence Data Prostate - physiology Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Prostatic Neoplasms - physiopathology S100 Calcium-Binding Protein A4 S100 Proteins - genetics S100 Proteins - metabolism |
| title | DNA methylation and immunohistochemical analysis of the S100A4 calcium binding protein in human prostate cancer |
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