Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels
Labeo rohita, commonly called rohu is one of the most important fish species for aquaculture in India. Digestive enzyme response and metabolic profile of fingerling L. rohita to different dietary crude protein (CP) levels (viz. 25, 30, 35 and 40%) were studied in an attempt to optimize a practical d...
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| Veröffentlicht in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2007, Vol.146 (1), p.107-114 |
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| creator | Debnath, D. Pal, A.K. Sahu, N.P. Yengkokpam, S. Baruah, K. Choudhury, D. Venkateshwarlu, G. |
| description | Labeo rohita, commonly called rohu is one of the most important fish species for aquaculture in India. Digestive enzyme response and metabolic profile of fingerling
L. rohita to different dietary crude protein (CP) levels (viz. 25, 30, 35 and 40%) were studied in an attempt to optimize a practical diet formulation for this species. After 45 days of feeding, activity of digestive enzymes and metabolite concentrations were assayed. Amylase, lipase and alkaline phosphatase (ALP) activities were not influenced by the dietary protein, but proteolytic and acid phosphatase (ACP) activities varied (
P
<
0.05) between the treatments. Proteolytic activity showed a second order polynomial relationship with dietary crude protein (CP) as
Y
=
0.0734
X
2
+
4.937
X
−
68.37,
r
2
=
0.97. A positive correlation was observed between dietary CP and amylase (
r
2
=
0.78). All the metabolites except muscle glucose showed significant change corresponding to the dietary protein levels. Glucose and glycogen levels corresponded to the dietary carbohydrate levels. Muscle and plasma pyruvic acid increased as the crude protein in the diet increased, whereas liver pyruvic acid showed the opposite trend. Muscle protein content was not affected by dietary CP. Protein fractions in plasma (total protein, albumin and globulin) showed maximum values in 30% CP fed group. It is concluded that proteolytic activity and ACP are the major digestive enzymes responsive to dietary CP in
L. rohita fingerlings. Considering the cost effectiveness of the diet, and based on liver and plasma free amino acid levels and plasma protein fractions, 30% crude protein is recommended as the optimal dietary protein for
L. rohita fingerlings. |
| doi_str_mv | 10.1016/j.cbpb.2006.09.008 |
| format | Article |
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L. rohita to different dietary crude protein (CP) levels (viz. 25, 30, 35 and 40%) were studied in an attempt to optimize a practical diet formulation for this species. After 45 days of feeding, activity of digestive enzymes and metabolite concentrations were assayed. Amylase, lipase and alkaline phosphatase (ALP) activities were not influenced by the dietary protein, but proteolytic and acid phosphatase (ACP) activities varied (
P
<
0.05) between the treatments. Proteolytic activity showed a second order polynomial relationship with dietary crude protein (CP) as
Y
=
0.0734
X
2
+
4.937
X
−
68.37,
r
2
=
0.97. A positive correlation was observed between dietary CP and amylase (
r
2
=
0.78). All the metabolites except muscle glucose showed significant change corresponding to the dietary protein levels. Glucose and glycogen levels corresponded to the dietary carbohydrate levels. Muscle and plasma pyruvic acid increased as the crude protein in the diet increased, whereas liver pyruvic acid showed the opposite trend. Muscle protein content was not affected by dietary CP. Protein fractions in plasma (total protein, albumin and globulin) showed maximum values in 30% CP fed group. It is concluded that proteolytic activity and ACP are the major digestive enzymes responsive to dietary CP in
L. rohita fingerlings. Considering the cost effectiveness of the diet, and based on liver and plasma free amino acid levels and plasma protein fractions, 30% crude protein is recommended as the optimal dietary protein for
L. rohita fingerlings.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/j.cbpb.2006.09.008</identifier><identifier>PMID: 17112756</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animal Feed ; Animal Nutritional Physiological Phenomena - drug effects ; Animals ; Carps - physiology ; Correlation matrix ; Crude protein ; Dietary Proteins - pharmacology ; Digestive enzymes ; Fish Proteins - metabolism ; Freshwater ; Growth ; Hydrolases - metabolism ; Labeo rohita ; Metabolites</subject><ispartof>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2007, Vol.146 (1), p.107-114</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-c3dc23acdeb198cf69826ebf4733fd503c69952cb457895a149930c976eeb0213</citedby><cites>FETCH-LOGICAL-c385t-c3dc23acdeb198cf69826ebf4733fd503c69952cb457895a149930c976eeb0213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1096495906002673$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17112756$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Debnath, D.</creatorcontrib><creatorcontrib>Pal, A.K.</creatorcontrib><creatorcontrib>Sahu, N.P.</creatorcontrib><creatorcontrib>Yengkokpam, S.</creatorcontrib><creatorcontrib>Baruah, K.</creatorcontrib><creatorcontrib>Choudhury, D.</creatorcontrib><creatorcontrib>Venkateshwarlu, G.</creatorcontrib><title>Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels</title><title>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</title><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><description>Labeo rohita, commonly called rohu is one of the most important fish species for aquaculture in India. Digestive enzyme response and metabolic profile of fingerling
L. rohita to different dietary crude protein (CP) levels (viz. 25, 30, 35 and 40%) were studied in an attempt to optimize a practical diet formulation for this species. After 45 days of feeding, activity of digestive enzymes and metabolite concentrations were assayed. Amylase, lipase and alkaline phosphatase (ALP) activities were not influenced by the dietary protein, but proteolytic and acid phosphatase (ACP) activities varied (
P
<
0.05) between the treatments. Proteolytic activity showed a second order polynomial relationship with dietary crude protein (CP) as
Y
=
0.0734
X
2
+
4.937
X
−
68.37,
r
2
=
0.97. A positive correlation was observed between dietary CP and amylase (
r
2
=
0.78). All the metabolites except muscle glucose showed significant change corresponding to the dietary protein levels. Glucose and glycogen levels corresponded to the dietary carbohydrate levels. Muscle and plasma pyruvic acid increased as the crude protein in the diet increased, whereas liver pyruvic acid showed the opposite trend. Muscle protein content was not affected by dietary CP. Protein fractions in plasma (total protein, albumin and globulin) showed maximum values in 30% CP fed group. It is concluded that proteolytic activity and ACP are the major digestive enzymes responsive to dietary CP in
L. rohita fingerlings. Considering the cost effectiveness of the diet, and based on liver and plasma free amino acid levels and plasma protein fractions, 30% crude protein is recommended as the optimal dietary protein for
L. rohita fingerlings.</description><subject>Animal Feed</subject><subject>Animal Nutritional Physiological Phenomena - drug effects</subject><subject>Animals</subject><subject>Carps - physiology</subject><subject>Correlation matrix</subject><subject>Crude protein</subject><subject>Dietary Proteins - pharmacology</subject><subject>Digestive enzymes</subject><subject>Fish Proteins - metabolism</subject><subject>Freshwater</subject><subject>Growth</subject><subject>Hydrolases - metabolism</subject><subject>Labeo rohita</subject><subject>Metabolites</subject><issn>1096-4959</issn><issn>1879-1107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTmPFDEQRi0EYpeFP0CAHJFNU253-5BI0HJKI5FAbPko73rUx6ztGbT8ejyakcggqarg1VeyHyGvGXQMmHi367zbu64HEB3oDkA9IddMSb1hDOTTNoMWm0GP-oq8KGUHwBXj7Dm5YpKxXo7imjx8THdYajoixeX344yF2iXQGat165Q83ec1pgnpGunWOlxpXu9TtTSm5Q7z1GqhEQMNCWuhv1K9b2OMmHGp1OdDwFNExbTQCY84lZfkWbRTwVeXfkN-fv704_brZvv9y7fbD9uN52qsrQbfc-sDOqaVj0KrXqCLg-Q8hhG4F1qPvXfDKJUeLRu05uC1FIgOesZvyNtzbjv_cGhvNHMqHqfJLrgeihFKSwVC_RfsYRiYlLKB_Rn0eS0lYzT7nGabHw0DczJiduZkxJyMGNCmGWlLby7pBzdj-LtyUdCA92eg_Q0eE2ZTfMLFY0gZfTVhTf_K_wN7Sp4l</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Debnath, D.</creator><creator>Pal, A.K.</creator><creator>Sahu, N.P.</creator><creator>Yengkokpam, S.</creator><creator>Baruah, K.</creator><creator>Choudhury, D.</creator><creator>Venkateshwarlu, G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>2007</creationdate><title>Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels</title><author>Debnath, D. ; Pal, A.K. ; Sahu, N.P. ; Yengkokpam, S. ; Baruah, K. ; Choudhury, D. ; Venkateshwarlu, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-c3dc23acdeb198cf69826ebf4733fd503c69952cb457895a149930c976eeb0213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animal Feed</topic><topic>Animal Nutritional Physiological Phenomena - drug effects</topic><topic>Animals</topic><topic>Carps - physiology</topic><topic>Correlation matrix</topic><topic>Crude protein</topic><topic>Dietary Proteins - pharmacology</topic><topic>Digestive enzymes</topic><topic>Fish Proteins - metabolism</topic><topic>Freshwater</topic><topic>Growth</topic><topic>Hydrolases - metabolism</topic><topic>Labeo rohita</topic><topic>Metabolites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Debnath, D.</creatorcontrib><creatorcontrib>Pal, A.K.</creatorcontrib><creatorcontrib>Sahu, N.P.</creatorcontrib><creatorcontrib>Yengkokpam, S.</creatorcontrib><creatorcontrib>Baruah, K.</creatorcontrib><creatorcontrib>Choudhury, D.</creatorcontrib><creatorcontrib>Venkateshwarlu, G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Debnath, D.</au><au>Pal, A.K.</au><au>Sahu, N.P.</au><au>Yengkokpam, S.</au><au>Baruah, K.</au><au>Choudhury, D.</au><au>Venkateshwarlu, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels</atitle><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><date>2007</date><risdate>2007</risdate><volume>146</volume><issue>1</issue><spage>107</spage><epage>114</epage><pages>107-114</pages><issn>1096-4959</issn><eissn>1879-1107</eissn><abstract>Labeo rohita, commonly called rohu is one of the most important fish species for aquaculture in India. Digestive enzyme response and metabolic profile of fingerling
L. rohita to different dietary crude protein (CP) levels (viz. 25, 30, 35 and 40%) were studied in an attempt to optimize a practical diet formulation for this species. After 45 days of feeding, activity of digestive enzymes and metabolite concentrations were assayed. Amylase, lipase and alkaline phosphatase (ALP) activities were not influenced by the dietary protein, but proteolytic and acid phosphatase (ACP) activities varied (
P
<
0.05) between the treatments. Proteolytic activity showed a second order polynomial relationship with dietary crude protein (CP) as
Y
=
0.0734
X
2
+
4.937
X
−
68.37,
r
2
=
0.97. A positive correlation was observed between dietary CP and amylase (
r
2
=
0.78). All the metabolites except muscle glucose showed significant change corresponding to the dietary protein levels. Glucose and glycogen levels corresponded to the dietary carbohydrate levels. Muscle and plasma pyruvic acid increased as the crude protein in the diet increased, whereas liver pyruvic acid showed the opposite trend. Muscle protein content was not affected by dietary CP. Protein fractions in plasma (total protein, albumin and globulin) showed maximum values in 30% CP fed group. It is concluded that proteolytic activity and ACP are the major digestive enzymes responsive to dietary CP in
L. rohita fingerlings. Considering the cost effectiveness of the diet, and based on liver and plasma free amino acid levels and plasma protein fractions, 30% crude protein is recommended as the optimal dietary protein for
L. rohita fingerlings.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>17112756</pmid><doi>10.1016/j.cbpb.2006.09.008</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2007, Vol.146 (1), p.107-114 |
| issn | 1096-4959 1879-1107 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68978068 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal Feed Animal Nutritional Physiological Phenomena - drug effects Animals Carps - physiology Correlation matrix Crude protein Dietary Proteins - pharmacology Digestive enzymes Fish Proteins - metabolism Freshwater Growth Hydrolases - metabolism Labeo rohita Metabolites |
| title | Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels |
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