Intrastriatal hypoxanthine administration affects Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, hippocampus and cerebral cortex of rats
The aim of this study was to investigate the effects of a single intrastriatal injection of hypoxanthine, the major metabolite accumulating in Lesch–Nyhan disease, on Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, cerebral cortex and hippocampus of rats at different post‐in...
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| Veröffentlicht in: | International journal of developmental neuroscience 2006-11, Vol.24 (7), p.411-417 |
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| container_title | International journal of developmental neuroscience |
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| creator | Bavaresco, Caren Serra Chiarani, Fabria Wajner, Moacir Netto, Carlos Alexandre Souza Wyse, Angela Terezinha |
| description | The aim of this study was to investigate the effects of a single intrastriatal injection of hypoxanthine, the major metabolite accumulating in Lesch–Nyhan disease, on Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, cerebral cortex and hippocampus of rats at different post‐infusion periods. Adult Wistar rats were divided in two groups: (1) vehicle‐injected group (control) and (2) hypoxanthine‐injected group. For Na+,K+‐ATPase activity determination, the animals were sacrificed 3 h, 24 h and 7 days after drug infusion. For the evaluation of acetylcholinesterase and catalase activities, the animals were sacrificed 30 min, 3 h, 24 h and 7 days after hypoxanthine infusion. Results show regional and time dependent effects of hypoxanthine on Na+,K+‐ATPase, acetylcholinesterase and catalase activities. The in vitro effect of hypoxanthine on the same enzymes in striatum was also investigated. Results showed that hypoxanthine inhibited Na+,K+‐ATPase, but not the activities of acetylcholinesterase and catalase in rat striatum. We suggest that these modification on cerebral biochemical parameters (Na+,K+‐ATPase, acetylcholinesterase and catalase activities) induced by intrastriatal administration of hypoxanthine in all cerebral structures studied, striatum, hippocampus and cerebral cortex, could be involved in the pathophysiology of Lesch–Nyhan disease. |
| doi_str_mv | 10.1016/j.ijdevneu.2006.08.007 |
| format | Article |
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Adult Wistar rats were divided in two groups: (1) vehicle‐injected group (control) and (2) hypoxanthine‐injected group. For Na+,K+‐ATPase activity determination, the animals were sacrificed 3 h, 24 h and 7 days after drug infusion. For the evaluation of acetylcholinesterase and catalase activities, the animals were sacrificed 30 min, 3 h, 24 h and 7 days after hypoxanthine infusion. Results show regional and time dependent effects of hypoxanthine on Na+,K+‐ATPase, acetylcholinesterase and catalase activities. The in vitro effect of hypoxanthine on the same enzymes in striatum was also investigated. Results showed that hypoxanthine inhibited Na+,K+‐ATPase, but not the activities of acetylcholinesterase and catalase in rat striatum. We suggest that these modification on cerebral biochemical parameters (Na+,K+‐ATPase, acetylcholinesterase and catalase activities) induced by intrastriatal administration of hypoxanthine in all cerebral structures studied, striatum, hippocampus and cerebral cortex, could be involved in the pathophysiology of Lesch–Nyhan disease.</description><identifier>ISSN: 0736-5748</identifier><identifier>EISSN: 1873-474X</identifier><identifier>DOI: 10.1016/j.ijdevneu.2006.08.007</identifier><identifier>PMID: 17034984</identifier><language>eng</language><publisher>United States</publisher><subject>Acetylcholinesterase ; Acetylcholinesterase - metabolism ; Animals ; Catalase ; Catalase - metabolism ; Cerebral Cortex - drug effects ; Cerebral Cortex - enzymology ; Corpus Striatum - drug effects ; Corpus Striatum - enzymology ; Hippocampus - drug effects ; Hippocampus - enzymology ; Hypoxanthine ; Hypoxanthine - administration & dosage ; In Vitro Techniques ; Intrastriatal injection ; Lesch–Nyhan ; Metabolic disease ; Na+, K+‐ATPase ; Rats ; Rats, Wistar ; Sodium-Potassium-Exchanging ATPase - metabolism ; Time Factors</subject><ispartof>International journal of developmental neuroscience, 2006-11, Vol.24 (7), p.411-417</ispartof><rights>2006 ISDN</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3757-91661fe60c7ac4e6649bcb7d75dd149a889af2d19d29c84162520521f69131213</citedby><cites>FETCH-LOGICAL-c3757-91661fe60c7ac4e6649bcb7d75dd149a889af2d19d29c84162520521f69131213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.ijdevneu.2006.08.007$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.ijdevneu.2006.08.007$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17034984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bavaresco, Caren Serra</creatorcontrib><creatorcontrib>Chiarani, Fabria</creatorcontrib><creatorcontrib>Wajner, Moacir</creatorcontrib><creatorcontrib>Netto, Carlos Alexandre</creatorcontrib><creatorcontrib>Souza Wyse, Angela Terezinha</creatorcontrib><title>Intrastriatal hypoxanthine administration affects Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, hippocampus and cerebral cortex of rats</title><title>International journal of developmental neuroscience</title><addtitle>Int J Dev Neurosci</addtitle><description>The aim of this study was to investigate the effects of a single intrastriatal injection of hypoxanthine, the major metabolite accumulating in Lesch–Nyhan disease, on Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, cerebral cortex and hippocampus of rats at different post‐infusion periods. Adult Wistar rats were divided in two groups: (1) vehicle‐injected group (control) and (2) hypoxanthine‐injected group. For Na+,K+‐ATPase activity determination, the animals were sacrificed 3 h, 24 h and 7 days after drug infusion. For the evaluation of acetylcholinesterase and catalase activities, the animals were sacrificed 30 min, 3 h, 24 h and 7 days after hypoxanthine infusion. Results show regional and time dependent effects of hypoxanthine on Na+,K+‐ATPase, acetylcholinesterase and catalase activities. The in vitro effect of hypoxanthine on the same enzymes in striatum was also investigated. Results showed that hypoxanthine inhibited Na+,K+‐ATPase, but not the activities of acetylcholinesterase and catalase in rat striatum. We suggest that these modification on cerebral biochemical parameters (Na+,K+‐ATPase, acetylcholinesterase and catalase activities) induced by intrastriatal administration of hypoxanthine in all cerebral structures studied, striatum, hippocampus and cerebral cortex, could be involved in the pathophysiology of Lesch–Nyhan disease.</description><subject>Acetylcholinesterase</subject><subject>Acetylcholinesterase - metabolism</subject><subject>Animals</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - enzymology</subject><subject>Corpus Striatum - drug effects</subject><subject>Corpus Striatum - enzymology</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - enzymology</subject><subject>Hypoxanthine</subject><subject>Hypoxanthine - administration & dosage</subject><subject>In Vitro Techniques</subject><subject>Intrastriatal injection</subject><subject>Lesch–Nyhan</subject><subject>Metabolic disease</subject><subject>Na+, K+‐ATPase</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Time Factors</subject><issn>0736-5748</issn><issn>1873-474X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFSqv2HQS7MSxnWXVDjBDNbBoETvL49xoPMoftlM6Ox6Bh-DJeBIcMogtK-va5zvHVwehC0pSSih_c0jtoYKHDsY0I4SnRKaEiCdoQaXIEybYl6doQUTOk0IweYZeeH8ghBQFYc_RGRUkZ6VkC_Rz3QWnfXBWB93g_XHoH3UX9rYDrKvWdja-6WD7Duu6BhM83urL5YfLX99_XN190h6WWBsIx8bs-yZSPkD0i3BXYTN5_hlMsA82WPDYdnhOG9sl3tth6I1uh9HPADjYufgP07sAj7ivcQz3L9GzWjceXp3Oc3T_dnV3_T65_fhufX11m5hcFCIpKee0Bk6M0IYB56zcmZ2oRFFVlJVaylLXWUXLKiuNZJRnRUaKjNa8pDnNaH6OXs--g-u_jnEV1VpvoGl0B_3oFZel4AUVUchnoXG99w5qNTjbandUlKipH3VQf_tRUz-KSBX7ieDFKWHctVD9w06FRMF6FnyzDRz_01Ztbrab9eZm9Xm7up_uiZzCfgOuwKos</recordid><startdate>200611</startdate><enddate>200611</enddate><creator>Bavaresco, Caren Serra</creator><creator>Chiarani, Fabria</creator><creator>Wajner, Moacir</creator><creator>Netto, Carlos Alexandre</creator><creator>Souza Wyse, Angela Terezinha</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200611</creationdate><title>Intrastriatal hypoxanthine administration affects Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, hippocampus and cerebral cortex of rats</title><author>Bavaresco, Caren Serra ; Chiarani, Fabria ; Wajner, Moacir ; Netto, Carlos Alexandre ; Souza Wyse, Angela Terezinha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3757-91661fe60c7ac4e6649bcb7d75dd149a889af2d19d29c84162520521f69131213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acetylcholinesterase</topic><topic>Acetylcholinesterase - metabolism</topic><topic>Animals</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Cerebral Cortex - drug effects</topic><topic>Cerebral Cortex - enzymology</topic><topic>Corpus Striatum - drug effects</topic><topic>Corpus Striatum - enzymology</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - enzymology</topic><topic>Hypoxanthine</topic><topic>Hypoxanthine - administration & dosage</topic><topic>In Vitro Techniques</topic><topic>Intrastriatal injection</topic><topic>Lesch–Nyhan</topic><topic>Metabolic disease</topic><topic>Na+, K+‐ATPase</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bavaresco, Caren Serra</creatorcontrib><creatorcontrib>Chiarani, Fabria</creatorcontrib><creatorcontrib>Wajner, Moacir</creatorcontrib><creatorcontrib>Netto, Carlos Alexandre</creatorcontrib><creatorcontrib>Souza Wyse, Angela Terezinha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of developmental neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bavaresco, Caren Serra</au><au>Chiarani, Fabria</au><au>Wajner, Moacir</au><au>Netto, Carlos Alexandre</au><au>Souza Wyse, Angela Terezinha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intrastriatal hypoxanthine administration affects Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, hippocampus and cerebral cortex of rats</atitle><jtitle>International journal of developmental neuroscience</jtitle><addtitle>Int J Dev Neurosci</addtitle><date>2006-11</date><risdate>2006</risdate><volume>24</volume><issue>7</issue><spage>411</spage><epage>417</epage><pages>411-417</pages><issn>0736-5748</issn><eissn>1873-474X</eissn><abstract>The aim of this study was to investigate the effects of a single intrastriatal injection of hypoxanthine, the major metabolite accumulating in Lesch–Nyhan disease, on Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, cerebral cortex and hippocampus of rats at different post‐infusion periods. Adult Wistar rats were divided in two groups: (1) vehicle‐injected group (control) and (2) hypoxanthine‐injected group. For Na+,K+‐ATPase activity determination, the animals were sacrificed 3 h, 24 h and 7 days after drug infusion. For the evaluation of acetylcholinesterase and catalase activities, the animals were sacrificed 30 min, 3 h, 24 h and 7 days after hypoxanthine infusion. Results show regional and time dependent effects of hypoxanthine on Na+,K+‐ATPase, acetylcholinesterase and catalase activities. The in vitro effect of hypoxanthine on the same enzymes in striatum was also investigated. Results showed that hypoxanthine inhibited Na+,K+‐ATPase, but not the activities of acetylcholinesterase and catalase in rat striatum. We suggest that these modification on cerebral biochemical parameters (Na+,K+‐ATPase, acetylcholinesterase and catalase activities) induced by intrastriatal administration of hypoxanthine in all cerebral structures studied, striatum, hippocampus and cerebral cortex, could be involved in the pathophysiology of Lesch–Nyhan disease.</abstract><cop>United States</cop><pmid>17034984</pmid><doi>10.1016/j.ijdevneu.2006.08.007</doi><tpages>7</tpages></addata></record> |
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| subjects | Acetylcholinesterase Acetylcholinesterase - metabolism Animals Catalase Catalase - metabolism Cerebral Cortex - drug effects Cerebral Cortex - enzymology Corpus Striatum - drug effects Corpus Striatum - enzymology Hippocampus - drug effects Hippocampus - enzymology Hypoxanthine Hypoxanthine - administration & dosage In Vitro Techniques Intrastriatal injection Lesch–Nyhan Metabolic disease Na+, K+‐ATPase Rats Rats, Wistar Sodium-Potassium-Exchanging ATPase - metabolism Time Factors |
| title | Intrastriatal hypoxanthine administration affects Na+,K+‐ATPase, acetylcholinesterase and catalase activities in striatum, hippocampus and cerebral cortex of rats |
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