Role of the sigma factor in transcription initiation in the absence of core RNA polymerase
Sigma factors (sigmas) are bacterial transcription factors that bind core RNA polymerase (RNAP) and direct transcription initiation at cognate promoter sites. However, most of their functions have been investigated in the context of RNAP. This has made the exact function of sigma, and the importance...
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| Veröffentlicht in: | Cell 2006-10, Vol.127 (2), p.317-327 |
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| creator | Hsu, Hsin-Hsien Chung, Kuei-Min Chen, Tsung-Ching Chang, Ban-Yang |
| description | Sigma factors (sigmas) are bacterial transcription factors that bind core RNA polymerase (RNAP) and direct transcription initiation at cognate promoter sites. However, most of their functions have been investigated in the context of RNAP. This has made the exact function of sigma, and the importance of core RNAP in modulating sigma function, ambiguous. Here we identify a Bacillus subtilis mutant sigma(A) that is independently capable of specific binding and melting of the promoter DNA. Interestingly, specific and independent promoter binding of sigma is sufficient for the temperature- and Mg(2+)-independent melting of promoter DNA around the transcription start site, in contrast to the temperature- and Mg(2+)-dependent melting by RNAP around the promoter -10 element. Thus core RNAP is able to negatively modulate the sigma-initiated melting of the transcription start site and, by sensing the changes in temperature and Mg(2+) concentration, to regulate the efficiency of promoter -10 melting. |
| doi_str_mv | 10.1016/j.cell.2006.08.042 |
| format | Article |
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However, most of their functions have been investigated in the context of RNAP. This has made the exact function of sigma, and the importance of core RNAP in modulating sigma function, ambiguous. Here we identify a Bacillus subtilis mutant sigma(A) that is independently capable of specific binding and melting of the promoter DNA. Interestingly, specific and independent promoter binding of sigma is sufficient for the temperature- and Mg(2+)-independent melting of promoter DNA around the transcription start site, in contrast to the temperature- and Mg(2+)-dependent melting by RNAP around the promoter -10 element. 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However, most of their functions have been investigated in the context of RNAP. This has made the exact function of sigma, and the importance of core RNAP in modulating sigma function, ambiguous. Here we identify a Bacillus subtilis mutant sigma(A) that is independently capable of specific binding and melting of the promoter DNA. Interestingly, specific and independent promoter binding of sigma is sufficient for the temperature- and Mg(2+)-independent melting of promoter DNA around the transcription start site, in contrast to the temperature- and Mg(2+)-dependent melting by RNAP around the promoter -10 element. Thus core RNAP is able to negatively modulate the sigma-initiated melting of the transcription start site and, by sensing the changes in temperature and Mg(2+) concentration, to regulate the efficiency of promoter -10 melting.</description><subject>Amino Acid Sequence</subject><subject>Amino Acids, Aromatic - chemistry</subject><subject>Bacillus Phages - genetics</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacillus subtilis - metabolism</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>DNA Footprinting</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - metabolism</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Magnesium - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nucleic Acid Denaturation</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Sigma Factor - chemistry</subject><subject>Sigma Factor - genetics</subject><subject>Sigma Factor - metabolism</subject><subject>Temperature</subject><subject>Transcription Initiation Site</subject><subject>Transcription, Genetic</subject><issn>0092-8674</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAYhD2AaCn8AQbkiS3hdfyReKwqvqQKpAoWlshxbHCVxMF2hv57Sikz051Oz91wCF0RyAkQcbvNtem6vAAQOVQ5sOIEzQFkkVWiZDN0HuMWACrO-RmakRI4Z5TO0fvGdwZ7i9OnwdF99ApbpZMP2A04BTVEHdyYnB_2gUtOHe2BV000gz7UtQ8Gb56XePTdrjdBRXOBTq3qork86gK93d-9rh6z9cvD02q5zsaCQcoqSWwljNW2VQ1oIjWXSilKG0kKyYWtoDSUKVY2jFDQElrWcialIbYFJugC3fzujsF_TSamunfx5w01GD_FWlSyFAUn_4JEUiZAyj14fQSnpjdtPQbXq7Cr_26j3xNqbkE</recordid><startdate>20061020</startdate><enddate>20061020</enddate><creator>Hsu, Hsin-Hsien</creator><creator>Chung, Kuei-Min</creator><creator>Chen, Tsung-Ching</creator><creator>Chang, Ban-Yang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20061020</creationdate><title>Role of the sigma factor in transcription initiation in the absence of core RNA polymerase</title><author>Hsu, Hsin-Hsien ; Chung, Kuei-Min ; Chen, Tsung-Ching ; Chang, Ban-Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p240t-891f86efcfdab0c19c59aaa33b912956f807e34a47b4130c90d4d5499e1fd0463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acids, Aromatic - chemistry</topic><topic>Bacillus Phages - genetics</topic><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacillus subtilis - metabolism</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>DNA Footprinting</topic><topic>DNA, Bacterial - metabolism</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - metabolism</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Electrophoretic Mobility Shift Assay</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Magnesium - chemistry</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nucleic Acid Denaturation</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Sigma Factor - chemistry</topic><topic>Sigma Factor - genetics</topic><topic>Sigma Factor - metabolism</topic><topic>Temperature</topic><topic>Transcription Initiation Site</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsu, Hsin-Hsien</creatorcontrib><creatorcontrib>Chung, Kuei-Min</creatorcontrib><creatorcontrib>Chen, Tsung-Ching</creatorcontrib><creatorcontrib>Chang, Ban-Yang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsu, Hsin-Hsien</au><au>Chung, Kuei-Min</au><au>Chen, Tsung-Ching</au><au>Chang, Ban-Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of the sigma factor in transcription initiation in the absence of core RNA polymerase</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>2006-10-20</date><risdate>2006</risdate><volume>127</volume><issue>2</issue><spage>317</spage><epage>327</epage><pages>317-327</pages><issn>0092-8674</issn><abstract>Sigma factors (sigmas) are bacterial transcription factors that bind core RNA polymerase (RNAP) and direct transcription initiation at cognate promoter sites. However, most of their functions have been investigated in the context of RNAP. This has made the exact function of sigma, and the importance of core RNAP in modulating sigma function, ambiguous. Here we identify a Bacillus subtilis mutant sigma(A) that is independently capable of specific binding and melting of the promoter DNA. Interestingly, specific and independent promoter binding of sigma is sufficient for the temperature- and Mg(2+)-independent melting of promoter DNA around the transcription start site, in contrast to the temperature- and Mg(2+)-dependent melting by RNAP around the promoter -10 element. Thus core RNAP is able to negatively modulate the sigma-initiated melting of the transcription start site and, by sensing the changes in temperature and Mg(2+) concentration, to regulate the efficiency of promoter -10 melting.</abstract><cop>United States</cop><pmid>17055433</pmid><doi>10.1016/j.cell.2006.08.042</doi><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Amino Acids, Aromatic - chemistry Bacillus Phages - genetics Bacillus subtilis Bacillus subtilis - genetics Bacillus subtilis - metabolism Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism DNA Footprinting DNA, Bacterial - metabolism DNA, Viral - genetics DNA, Viral - metabolism DNA-Directed RNA Polymerases - genetics DNA-Directed RNA Polymerases - metabolism Electrophoretic Mobility Shift Assay Gene Expression Regulation, Bacterial Magnesium - chemistry Molecular Sequence Data Mutation Nucleic Acid Denaturation Promoter Regions, Genetic - physiology Regulatory Sequences, Nucleic Acid Sigma Factor - chemistry Sigma Factor - genetics Sigma Factor - metabolism Temperature Transcription Initiation Site Transcription, Genetic |
| title | Role of the sigma factor in transcription initiation in the absence of core RNA polymerase |
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