Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential
In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and huma...
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| Veröffentlicht in: | Parasitology 2007-03, Vol.134 (3), p.359-367 |
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| creator | PARKAR, U. TRAUB, R. J. KUMAR, S. MUNGTHIN, M. VITALI, S. LEELAYOOVA, S. MORRIS, K. THOMPSON, R. C. A. |
| description | In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting. |
| doi_str_mv | 10.1017/S0031182006001582 |
| format | Article |
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J. ; KUMAR, S. ; MUNGTHIN, M. ; VITALI, S. ; LEELAYOOVA, S. ; MORRIS, K. ; THOMPSON, R. C. A.</creator><creatorcontrib>PARKAR, U. ; TRAUB, R. J. ; KUMAR, S. ; MUNGTHIN, M. ; VITALI, S. ; LEELAYOOVA, S. ; MORRIS, K. ; THOMPSON, R. C. A.</creatorcontrib><description>In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182006001582</identifier><identifier>PMID: 17052374</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Animals, Zoo - parasitology ; Australia ; Bacteria ; Biological and medical sciences ; Blastocystis ; Blastocystis - classification ; Blastocystis - genetics ; Blastocystis - isolation & purification ; Blastocystis Infections - parasitology ; Blastocystis Infections - transmission ; Cat Diseases - parasitology ; Cat Diseases - transmission ; Cats ; Comparative studies ; Culture ; Culture Media ; disease detection ; Dog Diseases - parasitology ; Dog Diseases - transmission ; Dogs ; feces ; Feces - parasitology ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Genes ; genotype ; Humans ; in vitro amplification ; Invertebrates ; Marsupialia - parasitology ; Methods ; Microbiology ; molecular characterization ; molecular epidemiology ; nucleotide sequences ; pathogen identification ; phylogeny ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Primates - parasitology ; ribosomal DNA ; Sequence Analysis, DNA ; Thailand ; wild animals ; zoo animals ; Zoonoses ; Zoonoses - parasitology ; Zoonoses - transmission</subject><ispartof>Parasitology, 2007-03, Vol.134 (3), p.359-367</ispartof><rights>2006 Cambridge University Press</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Cambridge University Press Mar 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-46f2689926b74bbdce1cfef41fe880c03891aacfd1aed0201579c01abb3d0a6b3</citedby><cites>FETCH-LOGICAL-c462t-46f2689926b74bbdce1cfef41fe880c03891aacfd1aed0201579c01abb3d0a6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182006001582/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,27901,27902,55603</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18497551$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17052374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PARKAR, U.</creatorcontrib><creatorcontrib>TRAUB, R. J.</creatorcontrib><creatorcontrib>KUMAR, S.</creatorcontrib><creatorcontrib>MUNGTHIN, M.</creatorcontrib><creatorcontrib>VITALI, S.</creatorcontrib><creatorcontrib>LEELAYOOVA, S.</creatorcontrib><creatorcontrib>MORRIS, K.</creatorcontrib><creatorcontrib>THOMPSON, R. C. A.</creatorcontrib><title>Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.</description><subject>Animals</subject><subject>Animals, Zoo - parasitology</subject><subject>Australia</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Blastocystis</subject><subject>Blastocystis - classification</subject><subject>Blastocystis - genetics</subject><subject>Blastocystis - isolation & purification</subject><subject>Blastocystis Infections - parasitology</subject><subject>Blastocystis Infections - transmission</subject><subject>Cat Diseases - parasitology</subject><subject>Cat Diseases - transmission</subject><subject>Cats</subject><subject>Comparative studies</subject><subject>Culture</subject><subject>Culture Media</subject><subject>disease detection</subject><subject>Dog Diseases - parasitology</subject><subject>Dog Diseases - transmission</subject><subject>Dogs</subject><subject>feces</subject><subject>Feces - parasitology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. 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A.</creator><general>Cambridge University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential</title><author>PARKAR, U. ; TRAUB, R. 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Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Genes</topic><topic>genotype</topic><topic>Humans</topic><topic>in vitro amplification</topic><topic>Invertebrates</topic><topic>Marsupialia - parasitology</topic><topic>Methods</topic><topic>Microbiology</topic><topic>molecular characterization</topic><topic>molecular epidemiology</topic><topic>nucleotide sequences</topic><topic>pathogen identification</topic><topic>phylogeny</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Primates - parasitology</topic><topic>ribosomal DNA</topic><topic>Sequence Analysis, DNA</topic><topic>Thailand</topic><topic>wild animals</topic><topic>zoo animals</topic><topic>Zoonoses</topic><topic>Zoonoses - parasitology</topic><topic>Zoonoses - transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PARKAR, U.</creatorcontrib><creatorcontrib>TRAUB, R. 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PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>17052374</pmid><doi>10.1017/S0031182006001582</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Animals, Zoo - parasitology Australia Bacteria Biological and medical sciences Blastocystis Blastocystis - classification Blastocystis - genetics Blastocystis - isolation & purification Blastocystis Infections - parasitology Blastocystis Infections - transmission Cat Diseases - parasitology Cat Diseases - transmission Cats Comparative studies Culture Culture Media disease detection Dog Diseases - parasitology Dog Diseases - transmission Dogs feces Feces - parasitology Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Genes genotype Humans in vitro amplification Invertebrates Marsupialia - parasitology Methods Microbiology molecular characterization molecular epidemiology nucleotide sequences pathogen identification phylogeny polymerase chain reaction Polymerase Chain Reaction - methods Primates - parasitology ribosomal DNA Sequence Analysis, DNA Thailand wild animals zoo animals Zoonoses Zoonoses - parasitology Zoonoses - transmission |
| title | Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential |
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